SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter plates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labeled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step, unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450/620 nm is read using an ELISA Microtiter plate reader.
Test principle: Enzyme-linked Immunosorbent Assay
Format: Microtiter plate with 96 wells
Incubation Time: 2 hours
Shelf Life (months): 6
Storage: 2–8°C
GSD SARS-CoV-2 ELISA IgA
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GSD SARS-CoV-2 ELISA IgA
The GSD SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.
Assay Features
- Separate Detection of IgG, IgM and IgA Antibodies
- Advantages of our Reagent System
∙ Total incubation under 2 hours
∙ Ready to use controls
∙ Assay procedure is easy to automate
Assay Specifications
| Article nr: |
GSD01-1029 |
| Nr. of Reactions |
96 |
| Sample matrix: |
Serum |
| Dilution: |
1:101 |
| Incubation time: |
30min/30min/30min |
| Incubation Temperature: |
Room temperature |
| Storage |
2-8 °C |
Reagents supplied
- 96 well breakable microtiter plate, coated with SARS-CoV-2 antigen, stabilized in a foil pouch, ready to use.
- Dilution Buffer, 2 x 50ml, with preservative and Tween 20, ready to use.
- Wash Concentrate 20X, 50ml, with preservative and Tween 20. Dilute with 1000ml distilled or deionized water before use.
- Negative Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Calibrator, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Positive Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Conjugate IgA 13.5ml, goat anti-human IgA antibodies with protein stabilizer and preservative, ready to use.
- Substrate Solution (TMB), 13.5ml, ready to use.
- Stop Solution, 7.5ml, ready to use.
GSD SARS-CoV-2 ELISA IgM
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GSD SARS-CoV-2 ELISA IgM
The GSD SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.
Assay Features
- Separate Detection of IgG, IgM and IgA Antibodies
- Advantages of our Reagent System
∙ Total incubation under 2 hours
∙ Ready to use controls
∙ Assay procedure is easy to automate
Assay Specifications
| Article nr: |
GSD01-1030 |
| Nr. of Reactions |
96 |
| Sample matrix: |
Serum |
| Dilution: |
1:101 |
| Incubation time: |
30min/30min/30min |
| Incubation Temperature: |
Room temperature |
| Storage |
2-8 °C |
Reagents supplied
- 96 well breakable microtiter plate, coated with SARS-CoV-2 antigen, stabilized in a foil pouch, ready to use.
- Dilution Buffer, 2 x 50ml, with preservative and Tween 20, ready to use.
- Wash Concentrate 20X, 50ml, with preservative and Tween 20. Dilute with 1000ml distilled or deionized water before use.
- Negative Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Calibrator, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Positive Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Conjugate IgM 13.5ml, goat anti-human IgM antibodies with protein stabilizer and preservative, ready to use.
- Substrate Solution (TMB), 13.5ml, ready to use.
- Stop Solution, 7.5ml, ready to use.
- RF-Adsorption solution, 4.5ml, ready to use.
GSD SARS-CoV-2 ELISA IgG
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GSD SARS-CoV-2 ELISA IgG
The GSD SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.
Assay Features
- Separate Detection of IgG, IgM and IgA Antibodies
- Advantages of our Reagent System
∙ Total incubation under 2 hours
∙ Ready to use controls
∙ Assay procedure is easy to automate
Assay Specifications
| Article nr: |
GSD01-1028 |
| Nr. of Reactions |
96 |
| Sample matrix: |
Serum |
| Dilution: |
1:101 |
| Incubation time: |
30min/30min/30min |
| Incubation Temperature: |
Room temperature |
| Storage |
2-8 °C |
Reagents supplied
- 96 well breakable microtiter plate, coated with SARS-CoV-2 antigen, stabilized in a foil pouch, ready to use.
- Dilution Buffer, 2 x 50ml, with preservative and Tween 20, ready to use.
- Wash Concentrate 20X, 50ml, with preservative and Tween 20. Dilute with 1000ml distilled or deionized water before use.
- Negative Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Calibrator, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Positive Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
- Conjugate IgG 13.5ml, goat anti-human IgM antibodies with protein stabilizer and preservative, ready to use.
- Substrate Solution (TMB), 13.5ml, ready to use.
- Stop Solution, 7.5ml, ready to use.