Quantify your gene's expression!

Targeted gene expression analysis with qPCR aims at the quantitative measurement of mRNA expression of defined target genes (e.g. in different genotypes, prior to and during drug therapy or after the administration of compound candidates in the pre-clinical phase of drug development).

Eurofins Genomics covers all steps in quantitative PCR (qPCR) - from the development of the experimental strategy to the final data analysis.

Based on the project, we use validated probes, customised probe designs or SYBR green assays for your quantitative PCR.

Eurofins Genomics offer target gene expression analysis with the following platforms:

• Roche LightCycler LC 480 II
• ABI TaqMan 7500
• Applied Biosystems 7900HT
• TaqMan Low Density Arrays (TLDA)
• Fluidigm’s Biomark HD and EP1 System

Strict confidentiality is ensured for every project. The high quality of our service is overseen by a professional quality management team. We are certified according to DIN EN ISO 9001:2008 and ISO 17025:2005 (D-PL-13372-01-00).

For further information or an individual quote, just contact us!

From the development of the experimental strategy to the final data analysis.

Benefit from our quantitative PCR (qPCR) analysis services:

• Consulting
  Comprehensive support by the selection of project oriented strategies.
• Experimental design
  The experimental design step starts with the generation of specific DNA primers and qPCR probes. Afterwards, gene specific DNA and RNA standards are cloned and synthesised.
• Sample preparation
  Isolation and purification of DNA and RNA from blood, tissue, cells, food, biotechnology products or buccal swabs.
• Assay development
  Optimisation of new real-time PCR assays reaching maximum levels of sensitivity, accuracy and reproducibility.
• Routine assay performance
  Professional performance of optimised real-time PCR assays. Eurofins Genomics performs assays with high throughput capacity and we even implemented micro fluidity formats in our processes.
• Data analysis
  We offer complete analysis of raw data for relative or absolute mRNA and / or gene quantifications. Clear results are determined by calculating the ratio of the gene of interest to the housekeeping gene.
• Documentation
  The experimental strategy, raw data and final results are summarised in a comprehensive report.

The high quality of our service is overseen by a professional quality management team. We are certified according to ISO 9001:2008 and accredited acc. to ISO 17025:2005 (D-PL-13372-01-00). These projects can also be performed according to GxP regulations.