How should I prepare my samples?

How do I submit my order?

It is easy. Go to our online ordering page.

1. Order in tube format
2. Order in plate format

How do I ship samples?

1. Dropbox - we have a nationwide network of dropboxes. If you want a box for your lab, let us know! Email GenomicsSupport@eurofins.com to request a box.

2. Free Digital Shipping Labels - digital shipping labels are provided free for a vast majority of sequencing orders. You can select the option for digital shipping label during checkout. Take a look at our sample submission options page for more details.

3. Ship the samples using your normal carrier - If using your own carrier, we highly recommend shipping overnight/next-day delivery to ensure the samples do not degrade in transit.

How do I get results?

Results are available to download from the order history page. You will be emailed when the results are ready as well.

How fast is your turnaround time?

Currently >90% of results are delivered the same day the samples are received. The exact time varies but typically it ranges between 5PM - 11PM EST.

Is there a minimum number of samples I must submit?

Any number of samples is welcome. There are no minimums or limitations.

How can I ship my samples to you?

Dropboxes are available in many locations. Also, Eurofins is the only sequencing company that offers free digital shipping labels on >95% of orders. Take a look at our sample submission page for details.

How accurate is it?

The equipment vendor reports accuracy of 99.3%. The research community has reported accuracy between 93-98%.

How does whole plasmid sequencing compare to Sanger sequencing?

There are pros and cons of every sequencing method. Sanger sequencing and Oxford Nanopore sequencing (ONT) are both methods for determining the sequence of nucleotides in a piece of DNA. Typically Sanger is consider the most accurate method for short-read sequencing and NGS is better for long-read sequencing.

Overall, the choice between Sanger sequencing and ONT will depend on the specific needs of the application. Both methods have their strengths and limitations, and the appropriate method will depend on factors such as the length and quality of the DNA sample, the desired level of accuracy, and the cost and availability of the necessary equipment.

	Pros	Cons
Short read sequencing (Sanger)	Widely used and well-established method High accuracy and precision Can be automated for high-throughput sequencing Chromatogram makes it easy to visually interpret results	Limited read length (typically up to 1000 base pairs) Requires relatively large amounts of high-quality DNA Requires reassembly for covering longer regions. Difficult to sequence repetitive regions without gaps and resolve large variations.
Long read sequencing (Whole Plasmid)	Long read lengths (up to several hundred thousand base pairs) Can sequence a wide range of sample types, including low-quality and degraded DNA No primer design required All data from one sample	Lower accuracy compared to Sanger sequencing, particularly for shorter reads More expensive per base pair compared to Sanger sequencing ·Cannot resolve single bases.

How should I prepare my samples?

Sample type	Size Category	Length	Concentration	Min volume	Price per sample
Plasmid	Regular	2.5 - 25 kb	30 ng/uL	≥10 uL	$15
	Large	25 - 125 kb	50 ng/uL	≥20 uL	$30
	XL	125 - 300 kb	50 ng/uL	≥40 uL	$60

Can I use my free barcodes on whole plasmid sequencing orders?

Absolutely. Using our free barcode labels is not required, but always an option.

Can I use my EVOcard to pay for my order?

Absolutely, yes.

Can you sequence my mixture of plasmids?

You can send it and we can sequence it, but we cannot predict or promise the analysis outcome. The customer would take on the risk of these orders.

How do I place an order?

It is easy. Go to our online ordering page.

Place order

Can I order through my institutions marketplace or B2B punchout?

We will be rolling out the new order page across portal sites, marketplaces, and punchouts very soon. At the moment, please submit orders on the main www.eurofinsgenomics.com website.

What is the coverage?

It really depends on the sample quality. We cannot guarantee the level of coverage at this time. A good sample submitted properly will typically yields hundreds or even thousands of sequencing reads. Consensus coverage depends on how many reads are full-length plasmids and how many, if any, degraded.

What data files is delivered?

1. .fasta file (for consensus data): we will provide a clean, complete consensus sequence for each plasmid.
   
2. .gbk GenBank file (for consensus data): a pLannotate map in the GenBank file format.
3. (OPTIONAL) .fastq file - raw data on reads. Email support to request fastq files.
4. Histogram file: the hisogram file provides a visual representation of the plasmid and raw read data for deeper insight into your samples (image).
5. .html pLannotate map (for consensus data): a plasmid map for each sample.
   
6. .csv confidence file with quality statistics.

What if my samples fail?

The probability of failure is low when using WPS, although it can still happen. If you wish to resequence a failed sample, contact Genomics Support. Unfortunately, we must charge for failed samples since it requires more time and energy than a normal run on the machine. If the sample fails a second time, all data points to a problem with the sample and we cannot resequence. In that scenario, we advise the customer to either send a new sample or troubleshoot the sample from their end.

Can I submit bacterial genome samples or Amplicons?

We will be adding this service soon but not yet.

Order Whole Plasmid Sequencing

Order in tube format

Order in plate format

Fast, accurate, and affordable long-read sequencing 

Confirm your full plasmids faster, more accurately, and more affordably than ever before. Whole genome sequencing offers long-read sequencing from one sample. It is suitable for large fragment DNA samples. NGS generation 3 technology has made it possible to quickly sequence whole plasmids in a fraction of the time and without the hassle of having to design and synthesize primers. Eurofins has developed a simple, user-friendly ordering process for sequencing plasmids and turning around results quickly.

Order in tube format

Order in plate format

Benefits

• Long read recovery of several kilobases
• Fast TAT compared to traditional primer walking and plasmid verification techniques
• Lower cost than traditional primer walking techniques
• Verification of the entire vector sequence
• Scalable--verify large plasmid constructs and large number of samples without impacting turnaround time.
• No primer design required. Easy set up.
• Available from one sample
• 5 - 4 million reads per Flow Cell (dependent on DNA quality and length)

Applications

Whole plasmid sequencing using NGS Gen 3 technology is a powerful and accurate method for characterizing and analyzing plasmids, which make it well suited for a wide range of applications.

• Plasmid Verification
• Resequencing of whole genomes
• Assembly of genomes
• Identification of taxonomic background
• Metagenomic analysis of long reads

Technology

Novel Nanopore technology is used to obtain very long sequences of several kilobases. It is a powerful method for characterizing and analyzing plasmids, which are small, circular pieces of DNA that can replicate independently of the genome. There are several key benefits to using ONT for whole plasmid sequencing:

1. High throughput: ONT allows for high-throughput sequencing of multiple samples in a single run, making it ideal for large-scale studies and projects.
2. Long read lengths: ONT generates long, continuous reads, which are particularly useful for sequencing large plasmids or for assembling complex plasmid structures.
3. High accuracy: ONT has a high accuracy rate, making it suitable for a wide range of applications, including plasmid engineering, quality control, and the identification of genetic mutations.
4. High resolution: ONT provides high-resolution data, allowing researchers to accurately identify and characterize even subtle differences in plasmid sequences.
5. Versatility: ONT is suitable for a wide range of sample types, including low-quality and degraded DNA, making it a versatile tool for plasmid analysis.

What Type of Sequencing Should I Use?

There are pros and cons of every sequencing method. Sanger sequencing and Oxford Nanopore sequencing (ONT) are both methods for determining the sequence of nucleotides in a piece of DNA. Typically Sanger is consider the most accurate method for short-read sequencing and NGS is better for long-read sequencing.

Overall, the choice between Sanger sequencing and ONT will depend on the specific needs of the application. Both methods have their strengths and limitations, and the appropriate method will depend on factors such as the length and quality of the DNA sample, the desired level of accuracy, and the cost and availability of the necessary equipment.