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  • Oligos
  • LocNA Primer

  Instructions / Help

Optimized sensitivity and specificity with LocNA (q)PCR Primers.
  • Sequence length from 10 - 35 bases
  • Up to 8 LocNA nts per primer are possible, but maximum 4 LocNA nts are recommended
  • Only DNA bases are allowed at the 3' end
  • Average synthesis yields of 4.5 OD resp. 20 nmole
  • Delivered lyophilized or concentration adjusted in tubes
  • Production takes 3 working days
Sequence info:
DNA bases: ACGT; LocNA bases: {ACGT} or {ACG}{T} or {AC}{GT} or {A}{C}{G}{T};
Use the IUB code for DNA wobble bases (equal amount of degenerated DNA bases)

How to order:
First, select your order entry method below. Use our xls template when uploading your primer sequences. More options are found in the next step.

Make use of the bubble icon to add a personal note to a primer; You can calculate the complement sequence with the arrow icon.

Enter primer name and sequence and press "Add to Cart".
 
  • Select your entry format
  • Select your individual oligos
 

LocNA Primers Entry Format



Number of oligos
 
Please select the number of primers / probes you want to order. You can always add more or leave lines empty on the next page.
XLS icon
Click on the XLS icon to download and complete the upload Excel form.
 
 
 
Define your oligos by using our Excel template above. Upload your file and press "Next".
 
 
Name and sequence can be pasted in one of the following formats (separated by a blank, tab, comma or semicolon):

Name1[blank]AC{GT}ACGTAC{GT}ACGT
Name2[TAB]GT{A} TAG T{A}G TAG
Name3, a{c}t gct gct {gc}c
Name4; act{gctg}aactgctgatgca

DNA bases: ACGT; LocNA bases: {ACGT} or {ACG}{T} or {AC}{GT} or {A}{C}{G}{T}
 
 
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