Summary

File type	Name	WPS	Amplicon	Bacterial Genome	AAV	Premium PCR	Custom Sequencing
.html	Analysis Report	Yes	Yes	Yes	Yes	no	no
.fasta, .gbk	Consensus sequence	Yes	Yes	Yes	Yes	no	no
.html	Plasmid map, pLannotate interactive map	Yes	Yes	Yes	Yes	no	no
.png	Real length histogram	Yes	Yes	Yes	Yes	no	no
.png	Coverage plot	Yes	Yes	Yes	Yes	no	no
.csv	Per-base data / Confidence file.csv	Yes	Yes	Yes	Yes	no	no
.fastq	Raw read sequenced	Yes	Yes	Yes	Yes	Yes	Yes
.html	Nanoplot Summary Report	Yes	Yes	Yes	Yes	no	no

File Types / Data Deliverables

Convenient report that contains the most important data about your sample including the map, histogram, coverage plot, deliverables and summary information.

The NanoPlot report is a unique file that contains a summary of the results, quality scores, and histograms.

Refined consensus sequence of the plasmid.

File type: .fasta, .gbk

Comprehensive, annotated plasmid map. For linear plasmids, the map may appears circular even though it is not. This is relic of the ONT assembly program.

File type: .html (pLannotate)

Visualization of raw sequencing read lengths. This distribution plot helps with sample characterization and troubleshooting. We provide several different histogram files to provide the full picture.

File type: .png

Coverage profile, showing sequencing depth across every base of the consensus sequence.

File type: .png / .gbk

Summary of each base. Includes the position, base, matches, mismatches, insertions, deletions, and more.

File type: .csv, .txt

Individual raw read sequences. For most sample types, the fastq files will only contain the sequences that align with the consensus.

File type: .fastq

A functional annotation (.gff) file is a standardized genome feature file that identifies and maps important biological elements within the sample, such as genes, coding regions, regulatory elements, and other genomic features. It allows researchers to interpret the biological function of the assembled sequence data alongside the raw nanopore reads.