Plasmid Verification Services
Confirm your plasmid constructs faster, more accurately and more affordably than ever before. Small and large constructs can now have the entire plasmid sequence done in a matter of days instead of weeks, without the hassle of having to design and synthesize primers. Eurofins uses a customized next-generation sequencing process to exceed the limitations, accuracy, and sensitivity of primer walking projects using Sanger sequencing techniques. Our quality-focused process uses unique molecular barcodes assigned to each sample to assure proper sample identification. Our bio-informatics pipeline provides accurate data assembly compared to a reference sequence, variant calling, and allele frequencies.
Request PV Project
*Minimum of 8-24 samples
Benefits
- Fast TAT compared to traditional primer walking techniques
- Lower cost than traditional primer walking techniques
- Verification of the entire vector sequence
- Verify large plasmid constructs and large number of samples without impacting turnaround time.
- No designing or purchasing of sequencing primers
- Sequence assembly provided
- Comprehensive report with variations from reference sequence and allele frequencies.
Service Details
- DNA only. Please provide 200ng in 20ul.
- Reference sequence for plasmid and construct required.
- Minimum of 24 samples in 96-well format required in most cases, 8 if we are currently doing a run.
- Sequencing for plasmids up to 50kb
- Turnaround time depends on the size and number of samples.
Deliverables
No Bioinformatics
- Run Summary/ Sequencing Results file
- Demultiplexed fastq files
With Bioinformatics
- Sequencing Results file
- Analysis Statistics file
- Variant Results file
- Consensus sequence fasta file
- Coverage Plots (upon request)
- Demultiplexed fastq files (upon request)
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Submission / Shipping Guidelines
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*Minimum of 24 samples in most cases, 8 if we are currently doing a run.