Eurofins Genomics has produced the qPCR probes and primers to fulfill millions of test kits around the globe, including both commercial assays and the CDC recommended assay. Researchers can order the individual sequences from the CDC assay or the pre-packaged assay at specified reaction quantities.
1. CDC Test Kit
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Order |
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2019-nCoV CDC RUO Primers and Probes
Kits include three individual tubes containing the CDC's published assays. Four sizes are available, based on the reaction count. Each tube comes premixed to the recommended concentration and volume.
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3. Individual Sequences
We can provide the primer and probe sequences recommended by the CDC to researchers working on the Coronavirus. The sequences were publicly provided by the CDC here. Researchers can order these primers and probes to create a panel in their lab, following the panel instructions from the CDC. These materials are provided for research purposes only. Eurofins Genomics does not claim that these materials are in any way endorsed or validated by the CDC.
*Enter the CDC sequences can be entered on the custom DNA oligo tube order page. Information can change quickly so we encourage all customers to cross check this sequence information against the CDC's documents found here.
Probes & Primers
Custom DNA plate order page
2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes
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Name
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Description
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Oligonucleotide Sequence (5’>3’)
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Label1
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Working
Conc.
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2019-nCoV_N1-F
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2019-nCoV_N1
Forward Primer
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5’-GAC CCC AAA ATC AGC GAA AT-3’
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None
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20 µM
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2019-nCoV_N1-R
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2019-nCoV_N1
Reverse Primer
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5’-TCT GGT TAC TGC CAG TTG AAT CTG-3’
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None
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20 µM
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2019-nCoV_N1-P
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2019-nCoV_N1
Probe
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5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3’
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FAM, BHQ-1
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5 µM
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2019-nCoV_N2-F
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2019-nCoV_N2
Forward Primer
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5’-TTA CAA ACA TTG GCC GCA AA-3’
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None
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20 µM
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2019-nCoV_N2-R
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2019-nCoV_N2
Reverse Primer
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5’-GCG CGA CAT TCC GAA GAA-3’
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None
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20 µM
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2019-nCoV_N2-P
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2019-nCoV_N2
Probe
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5’-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3’
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FAM, BHQ-1
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5 µM
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RP-F
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RNAse P Forward
Primer
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5’-AGA TTT GGA CCT GCG AGC G-3’
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None
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20 µM
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RP-R
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RNAse P Reverse
Primer
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5’-GAG CGG CTG TCT CCA CAA GT-3’
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None
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20 µM
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RP-P
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RNAse P
Probe
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5’-FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1-3’
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FAM, BHQ-1
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5 µM
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Note: Oligonucleotide sequences are subject to future changes as the 2019-Novel Coronavirus evolves.
Publications
- (2020) 2019 Novel Coronavirus (2019-nCoV), Wuhan, China. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
- (2020) 2019-Novel coronavirus (2019-nCoV) real-time rRT-PCR panel primers and probes. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
- (2020) Real-time RT-PCR panel for detection 2019-novel coronavirus. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
- (2020) Emergency use authorization. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
Disclaimer
These sequences are for research use only. It has not been tested or approved for clinical use.
- These sequences are for research use only. It has not been tested or approved for clinical use.
- These materials are impacted by the accuracy of the publicly shared SARS-CoV-2 genome sequences from the CDC or WHO. Eurofins Genomics is not responsible for issues pertaining to sequence design or accuracy of sequence design.
- Eurofins Genomics makes no claims on the performance of these sequences. The sequences and all instructional information have been taken from the CDC’s publicly shared documents and WHO information and produced to the highest quality standards.
- These materials can be impacted by many variables and uncontrolled processes, including factors pertaining to the customer’s lab and data analysis.
- Due to the urgency of this crisis, this product lacks the usual set of compliance specifications such as stability tests, shelf life studies, storage conditions, and other data.
- Eurofins Genomics is not, and does not claim to be, a primary, preferred or recommended source of the CDC or WHO coronavirus primer-probe panel. Eurofins Genomics is independently offering the sequences that were published by the CDC for use in coronavirus detection. These materials are for research use only. They have not been designed, tested, validated by CDC, nor are they in any way endorsed by the CDC or any other public health agency.
- This is not a COVID-19 test kit. The products covered on this page are reagents that can be used by a research organization or testing lab to develop their own COVID-19 test.
CDC INFORMATION FOR LABORATORIES
Eurofins Genomics is not, and does not claim to be, a primary, preferred or recommended source of the CDC coronavirus primer-probe panel. Eurofins Genomics is independently offering the sequences that were published by the CDC for use in coronavirus detection. These materials are for research use only. They have not been designed, tested, validated by CDC, nor are they in any way endorsed by the CDC or any other public health agency.