1. Standard Vectors
Vectors |
Size(bp) |
Antibiotic Resistance |
Multiple Cloning Site |
Sequence |
Map |
Copy in E. Coli |
pUCAmp |
3171 |
Ampicillin |
Yes |
Sequence |
Map |
High |
pUCKan |
3150 |
Kanamycin |
Yes |
Sequence |
Map |
High |
pUCAmpMinusMCS |
3284 |
Ampicillin |
No |
Sequence |
Map |
High |
pUCkanMinusMCS |
3305 |
Kanamycin |
No |
Sequence |
Map |
High |
pBR322 |
4369 |
Ampicillin |
Yes |
Sequence |
Map |
Low |
pBR322MinusMCS |
4441 |
Ampicillin |
No |
Sequence |
Map |
Low |
2. Custom Vectors
Eurofins Genomics also offers subcloning services into any cloning or expression vector of your choosing. Simply send us the vector into which you want us to subclone your insert. Please contact our technical support team for more information on our custom subcloning services.
When sending a vector for subcloning, please provide 5-10 µg of vector DNA, lyophilized or in solution (conc.: ~200ng/µl). Use a padded envelope and state the order number clearly, and mail your vector and a copy of your quotation or order confirmation to:
Blue Heron Bio
22310 20th Ave SE #100
Bothell, WA 98021 USA
Please include the following information with the submission:
- Map of the plasmid
- Detailed description of the plasmid (incl. info about sequencing primers)
- Complete DNA sequence in text format
- If it is an unmodified commercially available plasmid: supplier and info if it is the original plasmid
- If it is a modified plasmid: detailed description of the modifications (incl. ab1 files and gel picture)
3. Expression Vectors
Our partner company, Blue Heron Biotech, is pleased to clone into any expression vectors selected by our customers. Setup of custom vectors is simple and easy on our secure website. We also offer OriGene’s PrecisionShuttle™ system for easy subcloning into a large selection of proven destination vectors for a variety of downstream applications. When ordering in Origene expression vectors, customers pay only the cloning and synthesis fee. This eliminates the need to purchase and provide your own vector. Destination vector choices include:
- Epitope tags (MYC, DDK, etc.) for protein purification detection
- Fluorescent tags (GFP, RFP, YFP, etc.) for protein visualization
- Various drug markers (neomycin, puromycin, etc.) for stable cell line establishment
- E. coli expression vectors
- Custom requests
View Expression Vectors