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1. Standard Vectors

 Vectors Size(bp)  Antibiotic Resistance  Multiple Cloning Site  Sequence  Map  Copy in E. Coli 
 pUCAmp  3171  Ampicillin  Yes Sequence  Map  High
 pUCKan  3150  Kanamycin  Yes  Sequence   Map  High
 pUCAmpMinusMCS  3284  Ampicillin  No  Sequence   Map  High
 pUCkanMinusMCS  3305  Kanamycin  No  Sequence   Map  High
 pBR322  4369  Ampicillin  Yes  Sequence   Map  Low
 pBR322MinusMCS  4441  Ampicillin  No  Sequence   Map  Low



2. Custom Vectors

Eurofins Genomics also offers subcloning services into any cloning or expression vector of your choosing. Simply send us the vector into which you want us to subclone your insert. Please contact our technical support team for more information on our custom subcloning services.

When sending a vector for subcloning, please provide 5-10 µg of vector DNA, lyophilized or in solution (conc.: ~200ng/µl). Use a padded envelope and state the order number clearly, and mail your vector and a copy of your quotation or order confirmation to:

Blue Heron Bio
22310 20th Ave SE #100
Bothell, WA 98021 USA

Please include the following information with the submission:

  • Map of the plasmid
  • Detailed description of the plasmid (incl. info about sequencing primers)
  • Complete DNA sequence in text format
  • If it is an unmodified commercially available plasmid: supplier and info if it is the original plasmid
  • If it is a modified plasmid: detailed description of the modifications (incl. ab1 files and gel picture)


3. Expression Vectors

Our partner company, Blue Heron Biotech, is pleased to clone into any expression vectors selected by our customers. Setup of custom vectors is simple and easy on our secure website. We also offer OriGene’s PrecisionShuttle™ system for easy subcloning into a large selection of proven destination vectors for a variety of downstream applications. When ordering in Origene expression vectors, customers pay only the cloning and synthesis fee. This eliminates the need to purchase and provide your own vector. Destination vector choices include:

  • Epitope tags (MYC, DDK, etc.) for protein purification detection
  • Fluorescent tags (GFP, RFP, YFP, etc.) for protein visualization
  • Various drug markers (neomycin, puromycin, etc.) for stable cell line establishment
  • E. coli expression vectors
  • Custom requests

View Expression Vectors