Eurofins Genomics' Sequencing Primer Design Tool uses Prime+ of the GCG Wisconsin Package originally written by Irv Edelman.
With the sequence primer design tool, you can design forward and reverse sequencing primers for a target of interest. The optimal parameters for a primer are considered under default conditions. You can change these parameters for a more tailored primer design.Target Sequence
Copy & paste the target sequence from an external source. You can enter the DNA sequence information as well as FASTA sequences (starting with an ">" followed by the name) with line breaks and blank spaces. The template sequence may contain ambiguous bases, but the design tool will not select primers complementary to any ambiguous sites on the template sequence.Design Parameters
Choose the sequencing direction first. The terms forward primer and reverse primer are used in the design tool and in the resulting output.
Click on the "Design Primers" button to get the specified number of appropriate sequencing primers.The results are scored according to the best predicted performance criteria. Selected primers can directly be saved in your shopping cart and ordered either as Sequencing Primer product or as primer synthesis along with your sequencing order.