Eurofins Genomics offers many more modifications in addition to the standard portfolio. Non-standard modifications are ordered as needed, which adds 1-3 days to the turnaround time. Below is a list of non-standard modifications. If you do not see the modification that you are looking for, contact us! Our chemists are always up for a challenge.
How to order non-standard modifications
If you would like to order a non-standard modification, contact us at GenomicsSupport@genomics.eurofins-info.com. Most non-standard modifications cannot be entered on the order pages, but we can handle these requests manually.
Attachment Mods
Amino, thiol, acrydite, and phosphate groups incorporated during synthesis allow post synthesis reactions for attaching a variety of labels or for coupling the oligo with other molecules.
Modifier [Legacy Designator]
|
Availability
|
Structure
|
3'-PT-Amino-Modifier C3 [AmC3-PT]
|
3' |
|
Amino Modifier C2 dT [AmC2-dT]
|
5', Internal |
|
AminoC6 dC [AmC6-dC]
|
5', Internal |
Image not available.
|
DNA Analogs
Molecules with similar structure to DNA often readily substitute in the DNA strand for a variety of experimental uses. Modifications in this category include inosine, uridine, wobbles, etc.
Modifier [Legacy Designator]
|
Availability
|
Structure
|
3-Nitropyrrole [NitPyr]
|
5', Internal |
|
5-Nitroindole [5-NitIdl]
|
3' |
|
5-Nitroindole [5-NitIdl]
|
5', Internal |
|
AP-dC [AP-dC]
|
5', Internal |
Image not available.
|
dK [dK]
|
5', Internal |
|
dP [dP]
|
3' |
|
dP [dP]
|
5', Internal |
|
dP + dK mixture [dPdK]
|
5', Internal |
|
Fluorophores
Fluorescent molecules offer a wide variety of applications requiring detection of hybridization including in situ probes, sequencing, and genotyping.
Modifier [Legacy Designator]
|
Availability
|
Structure
|
6-Carboxyfluorescein [6Fl]
|
5', Internal |
|
6-Carboxyrhodamine (ester) [CR6G]
|
5', 3', Internal |
|
Cyanine3 (ester) [Cyanine3es]
|
3' |
Image not available.
|
Cyanine3.5 (ester) [Cyanine35es]
|
3' |
Image not available.
|
Cyanine5 (ester) [Cyanine5es]
|
3' |
Image not available.
|
DY682 (replaces IRD700) [DY682]
|
5', 3' |
Image not available.
|
DY782 (replaces IRD800) [DY782]
|
5' |
Image not available.
|
Tamra (ester) - 5-isomer [TMR-5]
|
5', 3', Internal |
|
Tide Quencher 2 ester [TQ2es]
|
5', 3', Internal |
Image not available.
|
Mixed Bases
Standard bases used in combination to create a base comprised of a mixture of 2-4 bases (e.g. A/C or A/C/G). This category includes IUB Wobbles and custom wobbles.
Modifier [Legacy Designator]
|
Availability
|
Structure
|
Mix-1 MG [Mix-1]
|
5', Internal |
Image not available.
|
Quenchers
Quenchers are most commonly paired with specific fluorophores and are chosen based on their ability to absorb energy at the emission wavelength of the fluorophore.
Modifier [Legacy Designator]
|
Availability
|
Structure
|
5-Dabcyl [5-Dabcyl]
|
5' |
|
QSY 7 (ester) [QSY7]
|
5', 3', Internal |
|
Tide Quencher 4 [TQ4]
|
3' |
Image not available.
|
Modified Bases
Chemically modified bases can be used for structural studies and enhanced binding. This category includes Br-dU, 5-methyl dC, 5-iodo dU, 2-aminopurine, etc.
Modifier [Legacy Designator]
|
Availability
|
Structure
|
2-Aminopurine [2AmPur]
|
5', Internal |
|
5-Bromo dU [5Br-dU]
|
3' |
|
5-Bromo-dC [5Br-dC]
|
5', Internal |
|
5-Bromo-dU [5Br-dU]
|
5', Internal |
|
5-Iodo-dU [5I-dU]
|
5', Internal |
|
O6-phenyl-dI [6phnl-dI]
|
5', Internal |
|
Pyrrolo-dC [Pyrlo-dC]
|
5', Internal |
|
RNA Analogs
RNA analogs are useful for creating the chimeric DNA/RNA oligos commonly used in anti-sense studies.
Modifier [Designator]
|
Availability
|
Structure
|
5'OH-Methyl-C [5HOMedC]
|
5', Internal |
Image not available.
|
Pyrrolo-RNA-C [Pyrrolo-rC]
|
5', Internal |
Image not available.
|
Ribo A [rA]
|
5', Internal |
Image not available.
|
Ribo A [rA]
|
3' |
Image not available.
|
Ribo C [rC]
|
3' |
Image not available.
|
Ribo C [rC]
|
5', Internal |
Image not available.
|
Ribo G [rG]
|
3' |
Image not available.
|
Ribo G [rG]
|
5', Internal |
Image not available.
|
Ribo U [rU]
|
5', Internal |
Image not available.
|
Ribo U [rU]
|
3' |
Image not available.
|
Ribo-8OxrG [8Ox-rG]
|
5', Internal |
Image not available.
|
Miscellaneous
Miscellaneous modifications include phosphorothioate backbone modification, digoxigenin labeling, modified bases to prevent extension of the 3' terminus, cholesterol for cell delivery, puromycin (a tRNA mimc), etc.
Modifier [Designator]
|
Availability
|
Structure
|
3'-dA (chain terminator) [3-dA]
|
3' |
|
3'-dC (chain terminator) [3-dC]
|
3' |
|
3'-dG (chain terminator) [3-dG]
|
3' |
|
3'-dT (chain terminator) [3-dT]
|
3' |
|
5-Pyrine Cap [5-PyrCAP]
|
5' |
Image not available.
|
Cholesteryl-TEG [Chols]
|
3' |
|
Puromycin [Pmycn]
|
3' |
|