200 μL 96 well plate (>48 samples)

• Write the order number along the side of the plate using a thin sharpie marker.
• After the order number, write ONT for Oxford Nanopore Samples (whole plasmid) or an S for Sanger samples.

• Make sure all handwriting is legible. 
• Do not submit less than 48 samples per plate. 
• Do not skip wells. 
• Arrange samples by column (preferred.

• Heat seal is preferred. If using a roller on an adhesive seal, press down firmly and evenly across the entire plate to make sure it is securely sealed across all wells.
• It is critical that plates are sealed well to avoid leakage and cross contamination between wells.
  • Inspect the seal for bubbles. Bubbles can cause cross contamination if liquid can pass between wells.
  • Wrap plates individually with bubble wrap to protect them.
• Do not use cap strips on plates. These can cause cross contamination between wells when pulling them off.
• Avoid stacking multiple plates on top of each other. The wells from the top plate can piece the seal of the plate underneath.

Agar Plate

• Wrap the agar plate in parafilm to prevent leakage and place inside a ziplock bag.
  
  

Separate Sample Types into Separate Bags 

• It is important to separate Whole Plasmid and Sanger samples into different ziplock bags so the processing team can quickly sort packages and send the samples to the right sequencing group.
• Include the order confirmation page in each bag, with the order barcode and sample information visible through the bag.