Dual Labeled Probes with locked nucleic acids

LocNA probes and primers contain Locked Nucleic Acid bases which improves the stability and performance of qPCR assays. These modified bases provide a 2'-O, 4'-C methylene bridge which effectively "locks" the 2' oxygen to the 4' carbon. LocNA probes and primers are the optimal choice when designing highly-specific, shorter probes with challenging target sequences. This novel approach has opened up a broad range of possibilities for researchers working in qPCR.

Specifications

 

Benefits

  • Average synthesis yields of 15 nmole resp. 2 OD
  • Sequence length from 5 - 40 bases
  • Up to 15 LocNA bases per probe are possible
  • Only DNA bases are allowed at the 5' and 3' end
  • HPLC purified by default
  • Delivered lyophilized (dry down) or concentration adjusted in tubes
  • Production takes 5 working days
  • QC report incl. ESI
 
  • Greater stability in assays.
  • Higher performance in assays.
  • Allows for more flexibility and possibilities when working on challenging target sequences.
  • Especially effective in target regions with a low GC percentage, AKA shorter target sequences.
  • Enhanced accuracy, SNP, and hybridization.


Dye-Quencher Combinations

*Contact us for custom probe projects not shown below.

5' Reporter Abs [nm] Em [nm] 3' Quencher
FAM [FAM]
495 520 TAM, BHQ1, TQ2
TET [5TET]
521 536 TAM, BHQ1
JOE [JOE]
520 548 TAM, BHQ1, TQ2
HEX [5HEX]
535 556 BHQ1, BHQ2, TQ2, TQ3
Cyanine3 [Cy3]
552 570 BHQ1, BHQ2
ROX [ROX]
575 602 BHQ2, TQ3
Texas Red [TxRed]
583 603 BHQ2, TQ3
Cal Fluor 590 [CalFluor590]
569 606 BHQ2
Cal Fluor Red 610 [CalFluor610]
590 610 BHQ2
ATTO 647N [ATTO647N]
644 669 BHQ2
Cyanine5 [Cy5]
649 670 BHQ2, TQ3

Additional Information

When inputting wobble/degenerate bases in the Sequence Entry box, use IUPAC code rather than parentheses, e.g. (A/G), (A/G/C/T), etc.

Order LNA Probes

Order LNA Primers

 

Alternative Ordering Option

Eurofins Genomics recently launched a new custom probes order page which combines qPCR, MGB, and LNA probe options into one place.

Order Custom Probes

Pricing is located on the price list page, under modifications.

 

Locked Nucleic Acid (LNA) is a modified RNA nucleotide that plays a crucial role in molecular biology and biotechnology. The unique structure of LNA includes a methylene bridge connecting the 2'-oxygen of the ribose and the 4'-carbon, which "locks" the ribose in the 3'-endo conformation. This locked structure enhances the thermal stability of LNA-containing nucleic acids, resulting in increased melting temperatures (Tm) for LNA/DNA and LNA/RNA duplexes. The improved hybridization properties of LNA make it a powerful tool in various applications, including diagnostics, therapeutics, and molecular biology research.

LNA bases are incorporated into LNA probes, which are oligonucleotides designed for high-affinity and sequence-specific binding to complementary DNA or RNA sequences. The increased binding affinity and specificity of LNA probes stem from the enhanced stability conferred by the locked ribose conformation. This allows for shorter probes with higher specificity and reduced off-target binding, which is particularly advantageous in applications such as fluorescence in situ hybridization (FISH), real-time PCR, and antisense therapy.

In diagnostics, LNA probes are employed to detect specific nucleic acid sequences with high sensitivity and precision. For instance, in real-time PCR, LNA-modified primers and probes ensure robust and reliable amplification and detection of target sequences, even in complex samples or those with low target concentrations. In therapeutic contexts, LNA-modified oligonucleotides can be used to modulate gene expression by binding to target mRNA sequences, thereby preventing translation and promoting degradation. This makes LNA probes valuable in the development of gene silencing therapies for various genetic disorders and diseases.

Overall, the integration of LNA bases into oligonucleotides represents a significant advancement in nucleic acid chemistry, offering enhanced stability, specificity, and binding affinity. The versatility of LNA probes across diagnostic and therapeutic applications underscores their importance in advancing molecular medicine and biotechnology.

Eurofins Genomics is an accredited cGMP laboratory, compliant with all regulation regarding the manufacturing of oligonucleotides for IVD or ASR use. If you wish to order probes using the GMP process, contact our technical team to discuss the project.

Testimonials

“We have been struggling with oligo quality from other providers. The ones we received from you are far and away the highest quality we have observed and at much higher yields and uniformity than we have seen elsewhere. We are incredibly impressed with the quality and have already started recommending Eurofins to collaborators and other colleagues.”

Andrew A.

Andrew A.

Customer

“You make the best primers. I love that you're able to get them to me in 24 hours with next-day shipping on oligos <= 40 bp. Free shipping, too. I've used your service for 4 of our lab's upcoming papers, and the quality is outstanding.”

Ben C.

Ben C.

Customer

“I have been using Eurofins since the past 20 years as a Lab Manager of an academic research lab in Toronto. I have found Eurofins Oligo service to be excellent in all respects. Great job Team Eurofins :)”

Talat A.

Talat A.

Customer

“Best Oligo ordering system I've ever used, and I've been ordering oligos since 1992!”

Bobb PhD

Bobb PhD

Customer

“Always a pleasure doing business with you! Excellent service and turnaround times! I have worked with Eurofins for 10 years, ever since I was a graduate student. My current lab used to order oligos and sequencing from other suppliers but I convinced them to switch to Eurofins. I advocate for you regularly to all of my lab colleagues.”

Bina N.

Bina N.

Customer

“Easy to navigate and order oligos. I order multiple oligos at once, each with similar specifications—purification, scale, and more—but with different sequences. The ability to set the default values after entering the first oligo made the process much faster and easier. I especially liked that feature.”

Dara L.

Dara L.

Customer

“Excellent quality oligo synthesis services”

Mona

Mona

Customer

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