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  • OrderGeneStrands

  GeneStrand Gene Fragments — Order Help

GeneStrands gene fragments are linear dsDNA fragments that can be used in a wide-range of applications. Some features of the sequence can be challenging to any synthetic Gene/GeneStrand project. Our proprietary sequence optimization tool, GENEiusTM, when coupled with our oligo synthesis and assembly methods capabilities, allows us to assemble a wide-range of custom Genes and GeneStrands gene fragments that may be challenging for other suppliers. Nevertheless, for the methods followed at Eurofins Genomics, the following features in the sequence make the synthesis and assembly complex:
  • Stretches of direct or inverted repeats
  • Homopolymeric stretches
  • Stretches of sequence that can result in critical secondary structures
  • Stretches of sequence with very high or low GC content.
  • Stretches of sequences with minor differences in the sequence.
To check if the sequence of your linear dsDNA has segments with these features, simply copy and paste your sequence into the sequence field. Ensure that
  • the sequence only contains the four standard DNA bases - A, C, G, & T; any non-standard characters included in the sequence will be eliminated (without replacement) during sequence validation.
  • the sequence does not contain spaces; the sequence should be entered as a one continuous block of sequence.
Please note that every sequence is validated by GENEiusTM before being added to the cart. The identified features and the suggestions for resolving these sequence-specific features are also provided by GENEius. To optimize your sequence and test the capabilities of GENEius, we have a limited-feature edition of GENEius, GENEius Light, that can be accessed through this link. A guest login and password is provided. You can test your sequence and upload the optimized sequence on the GeneStrands gene fragments order editor.
 
  • Select your entry format
  • Define your GeneStrand(s)
 


Number of GeneStrand(s)
 
Select the number of GeneStrands you wish to order, more can always be added on the next page.
 
 
Acceptable delimiters include tab, space, colon and semicolon.
Paste in your GeneStrands with name(blank)sequence e.g.:

name1 ACGTACGTACGTACGT
name2 G*T*A* TAGTAGTAG
name3 act gct gct gcc

*Delimiters refer to a character or symbol that separates a character string, word, or data item.
XLS icon
Click on the XLS icon to download and complete the order upload form. Thank you.
 
 
 
PLEASE NOTE:
  • Acceptable file types include xls, xlsx, csv, and txt.
  • Sequences can only be ≤2000 base pairs long.
 
 
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