Real-time, long-read sequencing for AAV-ITR applications

Eurofins Genomics offers affordable, fast AAV sequencing using the Oxford Nanopore (ONT) sequencing platform, which is known for providing real-time, long-read sequencing. Traditional sequencing methods often struggle with the repetitive and GC-rich regions of AAV genomes, whereas ONT traverses these complexities, ensuring complete and accurate genome assemblies.

Furthermore, Eurofins Genomics can analyze multiple AAV samples in parallel. This type of scalability is critical for large-scale studies and clinical applications, enabling researchers to rapidly obtain high-quality sequencing data.

Pricing

What is AAV?

Adeno-associated virus (AAV) is popular gene transfer vector for gene therapy, amongst other applications. Gene therapy is used to treat genetic disorders by introducing corrected genes into the affected cells. However, it can be challenging to introduce a gene vector without inadvertently causing a new pathogen. AAV helps to solve this problem because it is a nonpathogenic virus with low immunogenicity.

Quality Control in Vector Production

Quality control is paramount in the production of AAV vectors for clinical use. Eurofins Genomics sequencing enables real-time monitoring of vector batches, ensuring consistency and purity. This capability reduces the risk of contamination and ensures that only high-quality vectors proceed to clinical trials.

Regulatory Compliance

Regulatory agencies require comprehensive characterization of gene therapy vectors. ONT’s detailed sequencing data supports regulatory submissions by providing robust evidence of vector integrity, purity, and consistency. This facilitates the approval process, accelerating the timeline from research to clinical application.

Applications

Vector Genome Integrity

Ensuring the integrity of AAV vector genomes is crucial for therapeutic efficacy. ONT’s long-read sequencing technology can detect and characterize structural variants, deletions, and insertions within the AAV genome, providing insights into vector stability and potency. This comprehensive analysis is pivotal for optimizing vector design and manufacturing processes.

Full-length Capsid Sequence Analysis

The AAV capsid, composed of viral proteins, determines tropism and immunogenicity. Eurofins Genomics long-read sequencing can sequence full-length capsid genes without fragmentation, allowing for the precise identification of mutations, recombinations, and variants that may impact vector performance and safety.

Epigenetic Modifications

Epigenetic modifications such as methylation can influence AAV vector expression and packaging. Eurofins Genomics direct sequencing service can detect these modifications in native DNA without the need for bisulfite conversion, providing a more accurate and detailed epigenetic profile of AAV vectors.

ONT technology is well suited for AAV sequencing, offering accuracy, real-time data acquisition, and scalability. Its applications in vector genome integrity analysis, full-length capsid sequencing, and epigenetic profiling make it an indispensable tool for advancing gene therapy. Eurofins Genomics has combined the advantages of the ONT platform with our veteran sequencing expertise to offer an exceptional solution for AAV sequencing. By integrating our AAV sequencing service in your research and production pipelines, researchers and clinicians can enhance the development, safety, and efficacy of next-generation gene therapies.