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  • CustomPlateOligo

  Custom DNA Oligos in Plates — Order Help

Order Custom Oligos in Plates

You can place orders for oligos to be delivered in the plate format using one of the two options 1) Upload an excel sheet or 2) copy and paste the information in the format listed.

UPLOAD ORDER
You can access that excel template suitable for upload using the link below. We appreciate your cooperation in adhering to this format so we can deliver the best service possible.

Step 1
: Complete the Plate Upload order form. The minimum number of oligos per plate varies by product. For 4nmol small scale plates, the minimum is 48. For normal plates, the minimum is 24 oligos per plate. For orders with fewer oligos, please use the tube oligo order form. The dropdown options on the excel template provides the available modifications at the 5' and 3' termini. To order degenerate bases, you can use the single letter codes recognized by the IUB (international Union of Biochemistry).

IUB single letter code for mixed bases

Single Letter CodeBases Represented
B =C + G + T
D =A + G + T
H =A + C + T
K =T + G
M =A + C
N =A + C + G + T
Single Letter CodeBases Represented
R =A + G
S =G + C
W =A + T
V =A + C + G
Y =C + T
You can enter the sequences of the oligo in the linked excel sheet in any of the following formats. Note that you can select the arrangement of the well positions (by columns or rows); well positions are represented here 'by rows'.

PositionOligo Name5' ModsSequence3' Modsnote
A1Oligo1[AmC6]CATCATCATCATCATCAT some notes
A2Oligo2ACTACTACTACTACTACT
A3Oligo3cccaa[BioTEGi]gggtttnnnttt

Step 2: Specify Oligo Properties: Once the upload is validated, you can specify the purification, scale, and delivery format of the sample. If you choose for the oligos to be delivered in solution, please specify the volume.

Step 3: Check and Confirm: Finally, if you would like to edit your order, you can do so or simply confirm and proceed to checkout.

COPY AND PASTE
After selecting the copy and paste option, please proceed to paste your order in one of the formats described below.
 
  • Select your Entry Format
  • Specify Oligo Properties
  • Check and Confirm
 

Plate DNA Synthesis Entry Format


XLS icon
96-well Plate Upload Form
Click the XLS icon to download the template.
XLS icon
384-well Plate Upload Form
Click the XLS icon to download the template.
 
 
 
After correctly completing the upload form, additional options will be available on the next page.

 
 
Paste information about your oligos in the box above in the following order:

Well position, name, sequence.
e.g.:

A1 name1 ACGTACGTACGTACGT or
A2, name2, G*T*A*TAGTAGTAG or
A3; name3; actgctgctgcc

Accepted delimiters include tab, space, and semicolon. Delimiters refer to a character or symbol that separates a character string, word, or data item. Please do not include any space or other delimiters in the sequence.

 
 
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