INFORMATION

How to Order

Click the checkbox beside the primers you wish to order and hit "Add to Cart".

Summary

  • Our Standard Vector Primers are optimised and validated for perfect sequencing results
  • More than 80 predefined primers available
  • Typical yields of 25 nmole (5 OD)
  • Production in less than 1 working day

Specifications

Features of Standard PrimersDetails
Synthesis Scale50 nmole
Modificationsnone; only pre-defined sequences
Delivery FormatDry; in tubes
Available Lengths15–30 (predefined)
Production days2 days*

*please add the shipping days to the production days to determined the delivery days.

 

Information

In order to enable fast and convenient ordering of sequencing primers that are widely used to sequence inserts in standard cloning vectors, we have assembled a list of primer sequences that you can order with a single click. Simply select the primer and add it to the cart. We have also tailored the post-synthesis work flow to ensure that these primers have the quality that will provide the best sequencing results.

Typically, the quality of the primer is not critical for the amplification of sequences of interest when the amplified PCR product is analyzed by agarose gel electrophoresis. On the contrary, when the primers are used for sequencing a template, the quality of the primers is critical for the sucess.

Mutations are commonly identified by direct sequencing of the PCR product. The primer quality is therefore even more critical when you want to detect or verify single base variants:

  • Genotyping by sequencing
  • Microsatellite analysis
  • Verification of Site-Directed Mutagenesis experiments

Over the years, our scientists have sequenced several templates using the primer sequences below. These primers are added to your samples for no extra charge when indicated in your sequencing orders. We also encourage you to order these primers and prepare your 'premixed' samples. Amongst the different types of samples sequenced (excluding samples in ready2load plates), the premixed samples that require standard sequencing chemistry are processed at the most rapid pace and the sequence data is released earliest. To order any of the primers listed here, please use the Standard Primers link to avail a one-click ordering protocol for a HPSF-purified sequencing grade primer. The delivered primer can be used in over >500 sequencing reactions.

  Select Your Primers

Primer NameSequence (5' -> 3')Length [bases]Tm [°C]GC [%]PriceSelect
1492RCGG TTA CCT TGT TAC GAC TT2057.250$9.00
27FAGA GTT TGA TCC TGG CTC AG2055.245$9.00
3AOXGCA AAT GGC ATT CTG ACA TCC2157.948$9.00
5AOXGAC TGG TTC CAA TTG ACA AGC2157.948$9.00
96gIIICCC TCA TAG TTA GCG TAA CG2057.350$9.00
BGHrevTAG AAG GCA CAG TCG AGG1856.056$9.00
CMVfCGC AAA TGG GCG GTA GGC GTG2165.767$9.00
CYC1ReverseGCG TGA ATG TAA GCG TGA C1956.753$9.00
EF-1aForwardTCA AGC CTC AGA CAG TGG TTC2159.852$9.00
EGFPC1FGAT CAC TCT CGG CAT GGA C1958.858$9.00
EGFPC1RCAT TTT ATG TTT CAG GTT CAG GG2357.139$9.00
EGFPN1FGTC GTA ACA ACT CCG CCC1858.261$9.00
EGFPN1RGTC CAG CTC GAC CAG GAT G1961.063$9.00
GadForGGG ATG TTT AAT CCA CTA C1953.240$9.00
GadRevAAG AAA TTG AGA TGG TGC AC2053.240$9.00
Gal4ADTAC CAC TAC AAT GGA TG1747.941$9.00
Gal4BDTCA TCG GAA GAG AGT AG1750.447$9.00
GAPSEQFGTT CGA CCC CGC CTC GAT CC2065.570$9.00
GAPSEQRTGA CAC ACA TTC CAC AGG GTC2159.852$9.00
Gex-ForwardATA GCA TGG CCT TTG CAG G1956.753$9.00
Gex-ReverseGAG CTG CAT GTG TCA GAG G1958.858$9.00
GL2CTT TAT GTT TTT GGC GTC TTC CA2357.139$9.00
hU6FGAG GGC CTA TTT CCC ATG ATT2154.647.6$9.00
ITS1TCC GTA GGT GAA CCT GCG G1961.063$9.00
ITS4TCC TCC GCT TAT TGA TAT GC2055.245$9.00
M1396TGA GTT TCG TCA CCA GTA1851.444$9.00
M13FTGT AAA ACG ACG GCC AGT1853.750$9.00
M13F43AGG GTT TTC CCA GTC ACG ACG TT2362.452$9.00
M13RCAG GAA ACA GCT ATG ACC1853.750$9.00
M13R49GAG CGG ATA ACA ATT TCA CAC AGG2461.046$9.00
malEGGT CGT CAG ACT GTC GAT GAA GCC2466.158$9.00
mCherry-FCCC CGT AAT GCA GAA GAA GA2057.350$9.00
mCherry-RTTG GTC ACC TTC AGC TTG G1956.753$9.00
MycGCA TCA ATG CAG AAG CTG ATC TCA2461.046$9.00
pBABE3ACC CTA ACT GAC ACA CAT TCC2157.948$9.00
pBABE5CTT TAT CCA GCC CTC AC1752.853$9.00
pBabeFTGA CCT GGG AAG CCT TGG CT2061.460$9.00
pBabeRTTG CTG ACT AAT TGA GAT GCA TGC TTT2760.437$9.00
pBadFATG CCA TAG CAT TTT TAT CC2051.135$9.00
pBadRGAT TTA ATC TGT ATC AGG1846.933$9.00
pcDNAFGGC TAA CTA GAG AAC CCA CTG2159.852$9.00
pcDNARGGC AAC TAG AAG GCA CAG TC2059.355$9.00
petupATG CGT CCG GCG TAG A1654.363$9.00
pEXFGGA GCA GAC AAG CCC GTC AGG2165.767$9.00
pEXRCAG GCT TTA CAC TTT ATG CTT CCG GC2664.850$9.00
pFBACfTCC GGA TTA TTC ATA CCG TCC C2260.350$9.00
pFBACrCCT CTA CAA ATG TGG TAT GGC TG2360.648$9.00
pGL3forCTA GCA AAA TAG GCT GTC CC2057.350$9.00
pGLforGTA TCT TAT GGT ACT GTA ACT G2254.736$9.00
pGLrevCTT TAT GTT TTT GGC GTC TTC C2256.541$9.00
pJET12FCGA CTC ACT ATA GGG AGA GCG GC2366.061$9.00
pJET12RAAG AAC ATC GAT TTT CCA TGG CAG2459.342$9.00
pJET1FGCC TGA ACA CCA TAT CCA TCC2159.852$9.00
pJET1RGCA GCT GAG AAT ATT GTA GGA GAT C2561.344$9.00
pJETSEQFCGA CTC ACT ATA GGG AGA GCG GC2366.061$9.00
pJETSEQRAAG AAC ATC GAT TTT CCA TGG CAG2459.342$9.00
pQEforGTA TCA CGA GGC CCT TTC GTC T2262.155$9.00
pQErevCAT TAC TGG ATC TAT CAA CAG GAG2459.342$9.00
pShCMVfGGT CTA TAT AAG CAG AGC TG2055.245$9.00
pShCMVrGTG GTA TGG CTG ATT ATG ATC AG2358.944$9.00
RV3CTA GCA AAA TAG GCT GTC CCC2159.852$9.00
SeqL-ATCG CGT TAA CGC TAG CAT GGA TCT C2564.652$9.00
SeqL-BGTA ACA TCA GAG ATT TTG AGA CAC2457.638$9.00
SP6CAT TTA GGT GAC ACT ATA G1950.237$9.00
SV40pA-RGAA ATT TGT GAT GCT ATT GC2051.135$9.00
SV40-promoterTAT TTA TGC AGA GGC CGA GG2057.350$9.00
T3AAT TAA CCC TCA CTA AAG GG2053.240$9.00
T7TAA TAC GAC TCA CTA TAG GG2053.240$9.00
T7EEVATG TCG TAA TAA CCC CGC CCC G2264.059$9.00
T7PCS2TGT CTG GAT CTA CGT AAT ACG2155.943$9.00
T7termCTA GTT ATT GCT CAG CGG T1954.547$9.00
TrcHisFAAT CTG TGT GGG CAC TCG1856.056$9.00
TrcHisRCTT CTG CGT TCT GAT TTA ATC TG2357.139$9.00
V1-5GGA CTT TCC AAA ATG TCG1851.444$9.00
V5ACC GAG GAG AGG GTT AGG GA2061.460$9.00
VF2TGC CAC CTG ACG TCT AAG AA2057.350$9.00
VRATT ACC GCC TTT GAG TGA GC2057.350$9.00
XL39ATT AGG ACA AGG CTG GTG GG2059.355$9.00