In accordance with the Thanksgiving holiday, the sequencing lab will be open on Thursday 11/28 and closed Friday 11/29 and Saturday 11/30.

SimpleSeq™ is easy sequencing

SimpleSeq™ is a convenient, prepaid sequencing solution. Each kit includes 96 virtual sequencing credits and 96 barcoded tubes to submit samples. Turnaround time is the same as standard sequencing with 96% of results delivered the same day the samples arrive at the lab, typically by 10AM.

Simple

 

Sharable

SimpleSeq was designed to be easy. Everything you need is included in one neat package, including tubes, barcodes, prepaid shipping labels and more. Best of all, the sequencing itself is free since the kit comes with 96 prepaid sequencing credits.

 

SimpleSeq is extremely shareable. You can hand out tubes to labmates, share results, and view how many reactions each person used online. SimpleSeq enables scientific collaboration. See details below.

     

Smart

 

Speed

SimpleSeq is a smarter way to sequence. It removes the stress from planning and preparing for sequencing so you can invest that brainpower into what actually matters: science.   Barcoded tubes are processed first in the lab, before other tube types, which means SimpleSeq cut to the front of the line. Consider it the fast pass version of Sequencing.


How to Order

Simple Seq -Logo -for -web   Simple Seq -Premixed -Logo -for -web  
       

SimpleSeq Kit

 

SimpleSeq Premixed Kit

 

FlexReads
for refilling kit

How does SimpleSeq™ work?

 

 

 

Step SimpleSeq & SimpleSeq Premixed

Laptopsml

Step 1

Order Kit

SimpleSeq

  1. Log in.
  2. Order the SimpleSeq Kit (Standard or Premixed) using the control on the sidebar to the right.

Simple Seq -ordering -control -v3

   

Kiticon

Step 2

Submit Tubes

SimpleSeq - Submit tube order online

  1. Login to your account and go to the Sequencing order page,
  2. Enter the barcode located on the side of your SimpleSeq tube into the "No. Barcode" field. If the barcode is typed incorrectly or invalid, you may see an error message.
  3. Fill in the other required fields.
  4. In the cart, your SimpleSeq reactions go into our system at no cost.

SimpleSeq Premixed - Skip to next step

  1. SimpleSeq Premixed does not require an online order to process. Please ship the samples into the lab. We will take care of the rest.

 

 

 

   

Dropboxsml

Step 3

Mail Samples

Mail Your Samples

  1. Print a copy of your online order and attach it to your order.
  2. Prepare your samples in tubes and load them into the same shipping envelope.
    1. For standard SimpleSeq tubes, go to the Sample Submissions Guideline for information on how to prepare the samples.
    2. For SimpleSeq Premixed tubes, add template (5 μL) and primer (5 µL) to each SimpleSeqTM tube (total volume = 10 µL).
  3. Place tubes into a blue bag (supplied with the SimpleSeqTM kit) or custom ZipLock bags.
  4. After defining the reaction, please drop the material at a Eurofins Genomics dropbox (when available) or ship them from any nearby UPS dropbox. Digital shipping labels are available when you checkout and are free on a vast majority of orders.
   

 

 

Any account that submits tube barcodes from a kit will be automatically added to the kit. This allows multiple users to easily sync and share data.  Alternatively, users can manually share a kit from the sequencing dashboard page.

More Information on Sharing SimpleSeq

Sequencing is better shared. That is why SimpleSeq kits are so easy to share, activate, and use with multiple people. Anyone who submits a tube from a kit will be automatically ‘shared’ with that kit. Shared users can view the kit from their sequencing dashboard, track usage, and choose whether to share results or not. The dashboard provides full transparency of who is using each and every tube and reaction in the kit.

  1. Kits are automatically shared between accounts that have submitted barcodes from the kit. New users can view the kit on their sequencing dashboard as well. For Premixed kits, the owner must manually share the kit using the share control in the Sequencing Dashboard. However, SimpleSeq Premixed kits must be manually assigned and shared in the Sequencing Dashboard. If you wish to share SimpleSeq Premixed results, we recommend creating a group.
  2. The Sequencing Dashboard allows customers to track, view, and managing everything related to SimpleSeq. You can view how many reactions and barcodes are available on your account and see a visual map of each kit.
  3. FlexReads are virtual reactions that can be used with any kit. If a SimpleSeq user submits more than one reaction per tube, it leaves them with leftover tubes. FlexReads solves that problem by allowing users to purchase virtual reactions that can be used with any kit.
  4. Free barcodes no longer require manual activation. They are automatically activated once you enter them on the tube sequencing submission page.
  5. Assigning kits to another user is possible. Assigning a kit removes the kit from your account and links it to another account. Assigning is done on the sequencing dashboard page. This is valuable for purchasing managers and lab managers. They can purchase a kit, then assign it to the end user.

Activating Kits

Kits are automatically activated to the purchaser's account when the kit ships out of our facility. This usually takes 1-2 days. Furthermore, reactions will no longer overflow from one kit to another. Meaning reactions from one kit cannot be used with tubes from another kit. FlexReads have replaced this function with a more flexible solution.

Components of SimpleSeqTM kits

Kits Include Standard Kit
Premixed Kit
Free barcoded tubes 96 pcs 96 pcs
Shipping Envelopes Inquire 6
Shipping Labels 0 6
Barcodes (use on tubes for sending primers) 32 pcs 0

 

The SimpleSeqTM Premixed kit is suitable for the submission of ‘premixed’ samples. The SimpleSeqTM Standard kit allows for flexible and multiple sample submission options. You can pipette a template for multiple sequencing reactions into one SimpleSeqTM Standard barcoded tube. We’ll keep track of the number of sequencing ‘reactions’ you use. You can use the remaining barcoded SimpleSeqTM Standard tubes by simply purchasing flexpacks. The features of the two services are compared in table 2.

Comparison of the SimpleSeqTM services

Features Standard Premixed
96-Sequencing reactions
Results delivered by noon, even on Sat. (no additional fee)  ✔
Website Interactions
Online results storage  ✔*
User-friendly interface to manage/track inventory    ✔
Skip online submission  x  ✔
Online reaction submission and barcode reactivation    x
Sample submission and sequencing reaction options
Submit ‘premixed’ samples    ✔
Upgrade to power-read sequencing chemistry (additional fee)    x
Free standard primers    x
Custom primer synthesis (additional fee    x
Template for multiple sequencing reactions in one tube    x
Separate primer and template submission    x


Note:
that the flexible sample submission options allows you to submit samples for more than 96 sequencing reactions using SimpleSeqTM standard kit; however, additional fees will apply for every read after 96 sequencing reactions.  

*SimpleSeq Premixed results post to the buyer's account. For instance if a purchasing manager orders the premixed kit, your results will appear in their order history. However, you can create a group to enable results sharing to other users.

In summary, if you want to submit only ‘premixed’ samples, the SimpleSeqTM Premixed kit is the kit for you. If you want to avail the multiple benefits of flexible sample submission options, the SimpleSeqTM Standard kit and service, with flexible multiple options is what you need. We provide rapid overnight results for your sample, irrespective of the type of SimpleSeqTM kit used, shipped from anywhere in US, including Alaska and Hawaii, for the same low price!

Flex Read Logos _JSC_v 5 Done

FlexReads or 'Flexible' reads are virtual credits that can be used with any SimpleSeq kit. For SimpleSeq users that submit multiple reactions per tube, FlexReads provides a way to "refill" kits and use those leftover tubes. You can track how many FlexReads are available on your account in the sequencing dashboard.

  • 1-11 reactions needed - the system will automatically add the exact number of reactions needed to the cart, instead of requiring a phone call to "reactivate" an old tube.
  • >11 reactions needed - the system will automatically add a FlexPack to the cart. FlexPack sizes include 12, 24, 48, 96

Combining SimpleSeq with FlexReads

FlexReads and FlexPacks are prepaid, flexible reactions that can be used with any SimpleSeq kit. Since many people submit SimpleSeq tubes with multiple reactions in each tube, their kit reactions run out before their tubes are exhausted. With FlexReads, you can continue using your SimpleSeq tubes.New -flow

Overflow -Graphic -Legend

 

Order FlexReads & Info

 

 

When can I use FlexReads?

When you have fewer SimpleSeq tubes than the number of sequencing reations you require. Consider that you have four unused SimpleSeq tubes with you and 4 unused reactions. Moreover, you have four plasmids that need to be sequenced and would like to run 8 sequencing reactions, two sequencing reactions for each template. You can then load up your samples into four tubes (amount sufficient for 2 sequencing rxns), order us to use the free standard primers and submit the order. You will automatically be charged for the 4 extra reads - these will appear as FlexReads on your order summary.    

You can also purchase these FlexReads prior to placing a sequencing order. The tally of your FlexReads in the sequencing dashboard will be incremented with the number of FlexReads or the quantity of the FlexPack you have purchased. Subsequently, when you assemble you samples in the tubes from the SimpleSeqTM Standard kit, and place the order, you not be charged until the SimpleSeq rxns and FlexReads are consumed.   

Please note that these FlexReads are not tied to a specific barcodes of the SimpleSeq standard kit; these FlexReads can be used any SimpleSeq Standard Kit.

Safe & Secure Sample Processing

  • Barcode scanned entries enabled
  • Verification of typed barcode entries
  • Barcode tracking of all samples throughout our process
  • LIMS workflow managed with Geospiza GeneSifterTM software
  • When submitted for a single reaction, no tube transfers ensure direct template-to-results correlation

Every step of the order entry process is validated to ensure that your order can be processed quickly and accurately—virtually eliminating the possibility of sample ID typos. Once your sample is received, Geospiza GeneSifter™ Lab Edition integrates your online order entry with each laboratory step and transfers results information for quality review and secure online retrieval by the same account that placed the order.

 

Eco-Friendly packaging

We use recyclable and/or recycled materials in our all of our packaging, marketing materials, and products. Look for the green recycling symbol on each product package for details.

  • Paperless sample submission—no need to print an order form
  • Recycled/recyclable shipping & packaging materials
  • Rack Recycling Program

When the rack is empty you can reuse it, send it to a local recycling center that accepts polypropylene, or return the racks to us for recycling.

Eurofins Genomics,
Rack Recycling Program 12701,
Plantside  Drive, Louisville, KY, 40299

 

Tips & More Information

  • You can drop off samples at any UPS dropbox.
  • Digital shipping labels are available online, during checkout, when submitting tubes from a standard SimpleSeq Kits. These labels are free for a vast majority of orders, although it depends on the number of tubes.
  • A copy of the online order confirmation page must be enclosed with every shipment to ensure proper identification, tracking, and result retrieval.
  • An alternative option would be to submit your samples into a nearby dropbox, if one is located near you.
  • Results are typically received before noon the following day, via a secure online link in your account.
  • For general sample preparation guidelines, please refer to the sample preparation guidelines.

 

Terms & Conditions for Prepaid Items

  1. Prepaid DNA sequencing reactions can only be redeemed online at the website. No email or paper orders can be accepted for redemption of prepaid DNA sequencing reactions.
  2. Prepaid DNA sequencing reactions are standard DNA sequencing reactions only and cannot be redeemed or traded for Power Read, Large Clone, or Primer Walking reactions.
  3. SimpleSeq kits include the purchase of 96 prepaid sequencing reactions, and you will receive 96 2D bar-coded tubes for use in redeeming your reactions. These tubes are for use in submitting templates only. Primers cannot be submitted in these tubes.
  4. Prepaid DNA sequencing reactions can only be redeemed by using the 2D bar-coded tubes mentioned above for template submission.
  5. SimpleSeq kits and Prepaid plates come with 96 prepaid sequencing reactions each.  How these reactions are used and redeemed differs per product.
  6. For SimpleSeq, tubes remaining after all prepaid reactions have been redeemed can be used to purchase additional tube DNA sequencing reactions at the prepaid price. 
  7. Prepaid plates must be submitted at one time including all reactions.  If less than the total number of reactions, 96, is submitted, the remaining reactions are forfeited by the purchaser.
  8. You may redeem multiple reactions in a single tube or well (e.g. forward and reverse primer).
  9. After all prepaid reactions have been redeemed, the remaining 2D bar-coded tubes cannot be used for no-charge redemption of prepaid reactions. Additional purchases are required for use of these remaining tubes.
  10. All sales of prepaid DNA sequencing reactions are final. No refunds can be given for unused reactions or plates.
  11. 2D barcode labeled tubes and barcoded plates for prepaid DNA sequencing reactions cannot be replaced or refunded if lost or stolen.
  12. A minimum number of reactions is required for submitting prepaid plates. The minimum requirement is the same as all sequencing plates and set by the lab and updated periodically.
  13. All prepaid items expire after 3 years. 
  14. Free barcodes do not expire. 

 

 

Choose the best kit for you

Compare SimpleSeq and SimpleSeq Premixed below. Both guarantee results delivered the next morning, 96 reactions under 1 P.O., and you can submit tubes in any quantity at any time.

Simple Seq -Logo -for -web Simple Seq -Premixed -Logo -for -web
  • Submit samples online through the sequencing tube submission page.
  • Submit template for multiple reactions in 1 tube.
  • Full service or premixed reactions.
  • Free standard primers.

Most flexible and has better options for difficult templates.

  • Sample process as SimpleSeq standard kits, only the samples are premixed.
  • Submit tube barcodes online before shipping.
  • 1 premixed reaction per tube.
  • Results limited to buyer's account.

Best for customers that prefer to premix their samples and save cost.

 

Testing for Life

Eurofins is a global leader in lab testing. We can help you test almost anything. Learn more about how Eurofins is Testing for Life.

 

Testimonials

“It was good and convenient service. The sequencing results were great. I could just drop off the samples in the same building where I work. The results were very fast also, only one day." ★★★★★”

D. Prakoso

D. Prakoso

Customer

“Your sequencing reactions were perhaps the turning point in my work. Obtaining such good quality reads is rare. I have submitted many samples but this is was the best, we should explore all variable associated with the success to make it reproducible, quality, quantity of DNA to reagents used at Eurofins.”

Shyam G

Shyam G

Customer

“I've already strongly recommended Eurofins Genomics to a colleague in my department and they are now using the sequencing service. I was amazed at the turnaround time for prepaid plates and the quality far exceeds anything I've received from our in-house core facility. The quality and speed of the service combined with the price for the pre-paid plates has completely changed my lab's sequencing routine for the better.”

Herman M.

Herman M.

Academic

“Service has always been great!”

David L. Remington

David L. Remington

Academic

“Fast accurate service. Very reliable...excellent customer service. Every time I call you are helpful. I bet everything else you do is as good as your sequencing service.”

Andrew K.

Andrew K.

Researcher

“Always fast, always helpful, always the most rapid service provider.”

Ricardo B.

Ricardo B.

Research Scientist

“I am consistently surprised and pleased with how quickly you can provide sequencing data! I dropped my samples off late last evening at my local dropbox and my results were waiting in my inbox when I arrived at work this morning! My PI and I were just joking that we make a comment every time about how the results come so quickly. Maybe we just aren't used to such good customer service. Great job.”

Eva J.

Eva J.

Customer

“Sequencing is getting done faster than before, with same high quality. The sequencing quality has been constantly superior and I have been able to download the data at 8-9am the second day. Also customer service reps are extremely helpful and professional. Thanks!”

Wenzhen D.

Wenzhen D.

PROFESSOR

“We got the sequences back and are quite happy with the quality. One of the orders had amplicons that we have been sequencing routinely for many years and quality from Eurofins seems to be better. Also, our overall experience with Eurofins website, sample submission, email notifications and sequencing results was positive and user friendly. We are looking forward to sequencing with Eurofins.”

Anna M.

Anna M.

Research Scientist

“Brilliant sequencing services.”

I. Osekre

I. Osekre

BA, MBA

Related Products

Prepaid Plates

96 prepaid reactions in plate format

FlexReads

Virtual credits to use with SimpleSeq