How to submit SimpleSeq tubes online

 Overview of Process

Any account that submits tube barcodes from a kit will be automatically added to the kit. This allows multiple users to easily sync and share data.  Alternatively, users can manually share a kit from the sequencing dashboard page.

More Information on Sharing SimpleSeq

Sequencing is better shared. That is why SimpleSeq kits are so easy to share, activate, and use with multiple people. Anyone who submits a tube from a kit will be automatically ‘shared’ with that kit. Shared users can view the kit from their sequencing dashboard, track usage, and choose whether to share results or not. The dashboard provides full transparency of who is using each and every tube and reaction in the kit.

1. Kits are automatically shared between accounts that have submitted barcodes from the kit. New users can view the kit on their sequencing dashboard as well. For Premixed kits, the owner must manually share the kit using the share control in the Sequencing Dashboard. However, SimpleSeq Premixed kits must be manually assigned and shared in the Sequencing Dashboard. If you wish to share SimpleSeq Premixed results, we recommend creating a group.
2. The Sequencing Dashboard allows customers to track, view, and managing everything related to SimpleSeq. You can view how many reactions and barcodes are available on your account and see a visual map of each kit.
3. FlexReads are virtual reactions that can be used with any kit. If a SimpleSeq user submits more than one reaction per tube, it leaves them with leftover tubes. FlexReads solves that problem by allowing users to purchase virtual reactions that can be used with any kit.
4. Free barcodes no longer require manual activation. They are automatically activated once you enter them on the tube sequencing submission page.
5. Assigning kits to another user is possible. Assigning a kit removes the kit from your account and links it to another account. Assigning is done on the sequencing dashboard page. This is valuable for purchasing managers and lab managers. They can purchase a kit, then assign it to the end user.

Activating Kits

Kits are automatically activated to the purchaser's account when the kit ships out of our facility. This usually takes 1-2 days. Furthermore, reactions will no longer overflow from one kit to another. Meaning reactions from one kit cannot be used with tubes from another kit. FlexReads have replaced this function with a more flexible solution.

FlexReads or 'Flexible' reads are virtual credits that can be used with any SimpleSeq kit. For SimpleSeq users that submit multiple reactions per tube, FlexReads provides a way to "refill" kits and use those leftover tubes. You can track how many FlexReads are available on your account in the sequencing dashboard.

• 1-11 reactions needed - the system will automatically add the exact number of reactions needed to the cart, instead of requiring a phone call to "reactivate" an old tube.
• >11 reactions needed - the system will automatically add a FlexPack to the cart. FlexPack sizes include 12, 24, 48, 96

Combining SimpleSeq with FlexReads

FlexReads and FlexPacks are prepaid, flexible reactions that can be used with any SimpleSeq kit. Since many people submit SimpleSeq tubes with multiple reactions in each tube, their kit reactions run out before their tubes are exhausted. With FlexReads, you can continue using your SimpleSeq tubes.

Order FlexReads & Info

When can I use FlexReads?

When you have fewer SimpleSeq tubes than the number of sequencing reations you require. Consider that you have four unused SimpleSeq tubes with you and 4 unused reactions. Moreover, you have four plasmids that need to be sequenced and would like to run 8 sequencing reactions, two sequencing reactions for each template. You can then load up your samples into four tubes (amount sufficient for 2 sequencing rxns), order us to use the free standard primers and submit the order. You will automatically be charged for the 4 extra reads - these will appear as FlexReads on your order summary.    

You can also purchase these FlexReads prior to placing a sequencing order. The tally of your FlexReads in the sequencing dashboard will be incremented with the number of FlexReads or the quantity of the FlexPack you have purchased. Subsequently, when you assemble you samples in the tubes from the SimpleSeq^TM Standard kit, and place the order, you not be charged until the SimpleSeq rxns and FlexReads are consumed.   

Please note that these FlexReads are not tied to a specific barcodes of the SimpleSeq standard kit; these FlexReads can be used any SimpleSeq Standard Kit.

Choose the best kit for you! Compare SimpleSeq and SimpleSeq Premixed below. Both guarantee results the next morning, 96 prepaid reactions, and you can submit tubes in any quantity at any time.

Summary / Description	Fully custom or premixed samples. Multiple reactions per sample. Submit samples online. Free standard primers. Free primer barcodes included with kit.	Premixed samples only. Lower price than standard SimpleSeq kits.  Submit samples oneline. Must submit tube barcodes online before shipping. 1 premixed reaction per tube
Features
96-Sequencing reactions	✔	✔
Results delivered by noon, even on Sat. (no additional fee)	✔	✔
Website Interactions
Download results from the My Orders page	✔	✔
Sequencing dashboard provides a user-friendly interface to manage and track all your prepaid reactions	✔	✔
Convienent online order page to submit tubes	✔	x
Sample submission and sequencing reaction options
Submit ‘premixed’ samples	✔	✔
Upgrade to power-read sequencing chemistry (additional fee)	✔	✔
Free standard primers	✔	x
Custom primer synthesis (additional fee	✔	x
Template for multiple sequencing reactions in one tube	✔	x
Separate primer and template submission	✔	x

What is included with each kit

The SimpleSeq^TM Premixed kit is suitable for the submission of ‘premixed’ samples for a lower price. The SimpleSeq^TM Standard kit allows for flexible and multiple sample submission options. You can pipette a template for multiple sequencing reactions into one SimpleSeq^TM Standard barcoded tube. We’ll keep track of the number of sequencing ‘reactions’ you use. You can use the remaining barcoded SimpleSeq^TM Standard tubes by simply purchasing flexpacks. 

Note: that the flexible sample submission options allows you to submit samples for more than 96 sequencing reactions using SimpleSeq^TM standard kit; however, additional fees will apply for every read after 96 sequencing reactions.  

*SimpleSeq Premixed results post to the buyer's account. For instance if a purchasing manager orders the premixed kit, your results will appear in their order history. However, you can create a group to enable results sharing to other users.

In summary, if you want to submit only ‘premixed’ samples, the SimpleSeq^TM Premixed kit is the kit for you. If you want to avail the multiple benefits of flexible sample submission options, the SimpleSeq^TM Standard kit and service, with flexible multiple options is what you need. We provide rapid overnight results for your sample, irrespective of the type of SimpleSeq^TM kit used, shipped from anywhere in US, including Alaska and Hawaii, for the same low price!

Tips 

• You can drop off samples at any UPS dropbox.
• Digital shipping labels are available online, during checkout, when submitting tubes from a standard SimpleSeq Kits. These labels are free for a vast majority of orders, although it depends on the number of tubes.
• A copy of the online order confirmation page must be enclosed with every shipment to ensure proper identification, tracking, and result retrieval.
• An alternative option would be to submit your samples into a nearby dropbox, if one is located near you.
• Results are typically received before noon the following day, via a secure online link in your account.
• For general sample preparation guidelines, please refer to the sample preparation guidelines.

Terms & Conditions for Prepaid Items

1. Prepaid DNA sequencing reactions can only be redeemed online at the website. No email or paper orders can be accepted for redemption of prepaid DNA sequencing reactions.
2. Prepaid DNA sequencing reactions are standard DNA sequencing reactions only and cannot be redeemed or traded for Power Read, Large Clone, or Primer Walking reactions.
3. SimpleSeq kits include the purchase of 96 prepaid sequencing reactions, and you will receive 96 2D bar-coded tubes for use in redeeming your reactions. These tubes are for use in submitting templates only. Primers cannot be submitted in these tubes.
4. Prepaid DNA sequencing reactions can only be redeemed by using the 2D bar-coded tubes mentioned above for template submission.
5. SimpleSeq kits and Prepaid plates come with 96 prepaid sequencing reactions each.  How these reactions are used and redeemed differs per product.
6. For SimpleSeq, tubes remaining after all prepaid reactions have been redeemed can be used to purchase additional tube DNA sequencing reactions at the prepaid price. 
7. Prepaid plates must be submitted at one time including all reactions.  If less than the total number of reactions, 96, is submitted, the remaining reactions are forfeited by the purchaser.
8. You may redeem multiple reactions in a single tube or well (e.g. forward and reverse primer).
9. After all prepaid reactions have been redeemed, the remaining 2D bar-coded tubes cannot be used for no-charge redemption of prepaid reactions. Additional purchases are required for use of these remaining tubes.
10. All sales of prepaid DNA sequencing reactions are final. No refunds can be given for unused reactions or plates.
11. 2D barcode labeled tubes and barcoded plates for prepaid DNA sequencing reactions cannot be replaced or refunded if lost or stolen.
12. A minimum number of reactions is required for submitting prepaid plates. The minimum requirement is the same as all sequencing plates and set by the lab and updated periodically.
13. All prepaid items expire after 3 years. 
14. Free barcodes do not expire.