Wide range of scales, purification, and options for any project
We perform MALDI-TOF MS analysis on every custom DNA Oligo; infact we pioneered 100% MS testing in 1998. You can place an order for one or several oligos. For orders with large number of oligos, we recommend that you have the oligos delivered in plates.
| Features |
Oligos in Tubes |
Oligos in Plates |
| Synthesis Scales (in nmoles) |
10, 25, 50, 250, 1000, 2000, 5000, & 10,000 |
4, 10, 50, 250, 1000 |
| Modifications |
Several |
Fewer |
| Degenerate bases (see below) |
Single letter IUB code and predefined compositions |
| Available Oligo Lengths (in nt) |
5–125 |
| Synthesis Chemistry |
β-cyanoethyl Phosphoramidite coupling |
| Default Purification |
Salt-free purification |
| Other Purification Options |
HPLC, PAGE, 2-Step |
HPLC, PAGE |
| Delivery Formats |
Dry or in solution (normalized) |
Dry or in solution (normalized) |
Lengths of oligonucleotides available in tubes and plates at different synthesis scales
| Synthesis Scale (nmole) |
Available Lengths (in nts) |
| 10 |
5–60 |
| 25 |
5–80 |
| 50 |
5–90 |
| 250 |
5–125 |
| 1,000 |
5–125 |
| 10,000 |
5–100 |
Production process
The integration of the ecommerce site with the back-end laboratory information managment systems (LIMS) ensures that each synthesized oligo is tracked through the various steps in the production process. As soon as an oligo order is placed on the ecom site, the automated order confirmation email is sent and the details of the order is transferred to the production lab without any loss in the order data. An unique barcode assigned to each oligo order ensures that the production status of an oligo can be monitored at any given instance.
Custom DNA oligos at Eurofins Genomics are synthesized by coupling the relevant series of β-cyanoethyl phorphoramidites on pre-packed controlled-pore solid supports before being deprotected, de-salted, quantitated and being subject to quality control assessment. Typical coupling efficiencies acheived using our automated synthesis platforms are grater than 99%. The coupling efficiencies, when coupled with the length of the oligo sequence to be synthesized, essentially determine the yield of the oligo. Eurofins Genomics has optimized its synthesis scale and sequence length using its traditional high-throughput synthesis platforms to produce high-quality oligos of different lengths.
Yield
Go to the yield webpage for information about yields.