Wide range of scales, purification, and options for any project

We perform MALDI-TOF MS analysis on every custom DNA Oligo; infact we pioneered 100% MS testing in 1998. You can place an order for one or several oligos. For orders with large number of oligos, we recommend that you have the oligos delivered in plates.

Production process

The integration of the ecommerce site with the back-end laboratory information managment systems (LIMS) ensures that each synthesized oligo is tracked through the various steps in the production process. As soon as an oligo order is placed on the ecom site, the automated order confirmation email is sent and the details of the order is transferred to the production lab without any loss in the order data. An unique barcode assigned to each oligo order ensures that the production status of an oligo can be monitored at any given instance.

Estimated Turnaround Time for Saltfree, Unmodified Oligonucleotides

Measured in business days

Custom DNA oligos at Eurofins Genomics are synthesized by coupling the relevant series of β-cyanoethyl phorphoramidites on pre-packed controlled-pore solid supports before being deprotected, de-salted, quantitated and being subject to quality control assessment. Typical coupling efficiencies acheived using our automated synthesis platforms are grater than 99%. The coupling efficiencies, when coupled with the length of the oligo sequence to be synthesized, essentially determine the yield of the oligo. Eurofins Genomics has optimized its synthesis scale and sequence length using its traditional high-throughput synthesis platforms to produce high-quality oligos of different lengths.

Express Oligos Turnaround Time

Purification options available for oligos in tubes and plates based on the length of the oligo and the synthesis scale:

Please note that some oligos, particularly those containing modifications, are offered only with specific purification options. To see the yield of standard unmodified oligos based on the purification and length of the oligo, please visit the yields and purifications page.

Standard IUPAC/IUB single letter codes for degenerate bases
Degenerate bases in primers serve a useful purpose in amplifying homologous genes from other species; moreso when the amino acid sequence of the homologoes protein from the species is known. The presence of degeneracy in the bases of the primer sequence accommodates the potential difference in the genetic sequence and yields an amplified product. To order oligos with such degenerate bases in the sequence, include the single letter code for the base as recognized by IUPAC and IUB to incorporate equal quantities of each base represented by that letter at the desired positions. Please note that these degenerate bases are not available for the oligos synthesized at the 4 nmole synthesis scale.

Quality Control Processes

Quality controls processes are designed to evalaute both individual oligos or the total process.

Quality Control for Oligos

Every oligo is assayed using mass spectra. Shorter oligos (≤50 nts long) are typically evalauted using Matrix-Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry, while the longer oligos (≥50 nts) are analyzed by Electro-Spray Ionization (ESI) mass spectrometry. For oligos used in special applications, e.g., qPCR probes, purification and analysis is carried out using HPLC.

Quality Control for Production Processes

To assess the whole production process flow, orders for production control primers and probes are placed randomly each day. These orders proceed through the work-flow anonymously and are evaluated in qPCR reactions or assessed by our Quality Assurance team.