ePools

About ePools

Oligonucleotide pools are collections of short, single-stranded DNA sequences which are synthesized separately before being combined into a "pool." Pools are a versatile tool which serve a variety of applications in molecular biology, genetic research, and diagnostics. These pools consist of a large number of unique oligonucleotides, which may vary in sequence, length, or composition depending on the intended experiment. By providing a diverse set of oligos, researchers can quickly evaluate a wide range of genetic targets or conditions in a single experiment, making oligonucleotide pools indispensable in high-throughput applications such as gene expression profiling, RNA sequencing, and functional genomics studies.

Whether you're exploring gene synthesis, functional genomics, or high-throughput screening, our oligo pools deliver exceptional flexibility, quality, and scalability, ensuring you get the precise tools you need—every time.

Specifications

Specification	10 picomole per oligo	50 picomole per oligo
Min / Max number of oligos per pool	10 to 2,000 oligos	2 to 384 oligos
Oligo length restriction	40 – 200 bases
Modifications available	5' Phos [PHO]
Mixed bases / wobbles	R, Y, M, K, S, W, H, B, V, D, N
QC	None

 

Pricing

Number of Bases	Price
1-3300	$130.00 USD / flat rate per pool
3300 - 50,000	$0.04 / base
51,000 - 100,000	$0.03 / base

Modification	Price
5' Phosphate [PHO]	$1.83
PTO Bond *	$1.85

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How to Order

1. CLICK HERE to the ePool order page.

2. Upload the sequences using the provided excel template. Use the pool name column to separate pools.

 

Primary Application

One of the primary uses of oligonucleotide pools is in the field of CRISPR-based genome editing, where they are often employed as part of libraries for screening gene function. In such applications, the oligonucleotide pools can be synthesized to encode various guide RNAs, enabling the targeting of multiple genes or genomic regions simultaneously. This reduces the time and cost typically associated with individually designing and testing each sequence. Similarly, oligonucleotide pools are essential for RNA interference (RNAi) experiments, where they are used to deliver small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) to knock down specific gene expression across a broad spectrum of targets.

Beyond gene editing and gene silencing, oligonucleotide pools are also commonly utilized in diagnostics and therapeutic development. For instance, they can be used to develop multiplex assays for detecting multiple pathogens or genetic variations in a single test, streamlining diagnostic workflows and improving their efficiency. Additionally, in drug discovery, oligonucleotide pools are employed to identify novel therapeutic candidates by screening for compounds that interact with particular nucleic acid sequences, aiding in the development of oligonucleotide-based drugs such as antisense oligonucleotides (ASOs) or aptamers.

The design and synthesis of oligonucleotide pools are typically optimized for scalability, accuracy, and quality, ensuring that they can meet the specific needs of any project, whether for academic research, clinical diagnostics, or industrial applications. By leveraging the power of oligonucleotide pools, researchers can accelerate discovery, reduce costs, and gain deeper insights into complex biological processes.

Secondary Applications

• Gene Synthesis
  Speed up gene assembly with high-quality, custom oligo pools. Whether you're assembling full-length genes, synthetic operons, or gene libraries, our oligos ensure seamless integration and high accuracy.

• Next-Generation Sequencing (NGS)
  Tailored to your sequencing needs, our oligo pools are ideal for preparing target-specific probes or amplifying regions of interest. Get better results with custom sequences optimized for your NGS platform.

• Functional Genomics
  Leverage our oligo pools for high-throughput gene expression studies, RNA interference, or other genomic perturbation experiments. Designed for flexibility and scalability, our pools help you tackle a variety of functional genomics challenges.

 

What Makes Eurofins Different

Key Features of Our Oligo Pools

• High-Quality Synthesis: Our proprietary synthesis technology produces raw oligos with higher purity and coupling efficiency than other platforms, which allows us to achieve superior pool consistency.
• Flexible Pooling Options: Not seeing an option that you need? We partner with laboratories every day to create custom pools, no matter how large or small, to meet exact specifications. Contact us to discuss your custom project.
• Cost-Effective: Get exceptional quality at competitive prices, without compromising on performance.
• Multiple Uses: Perfect for CRISPR/Cas9 gene editing, next-generation sequencing, functional genomics, RNA interference (RNAi), and more.

Trusted Partner in Genomic Research

For over 30 years, Eurofins Genomics has been a trusted provider of custom oligonucleotide synthesis and genomics services to researchers worldwide. We offer a range of tailored solutions to meet the unique needs of your research, with an emphasis on supporting the scientific community, innovation, quality, and customer satisfaction.

Take your research to the next level with ePools — custom-made to fit your needs, delivered with speed, precision, and reliability.