In accordance with the Thanksgiving holiday, the sequencing lab will be open on Thursday 11/28 and closed Friday 11/29 and Saturday 11/30.

About Special Protocols

Special protocols refer to any non-standard process which is added to an order to further customize the final deliverable.

For instance, a commercial company developing a highly sensitive assay may require special processing steps to ensure the incoming oligos perform properly and consistently within the reaction. Other examples of special protocols include pooling, custom yield, stamping, and GMP documentation, amongst others. They are particularly useful in highly demanding industries such as molecular diagnostics (MDx), clinical, and NGS applications.

Almost all special protocols require some level of customization to meet the client’s needs, and we recommend contacting us to setup the initial workflow. Once the process is in place, customers can freely order these services online, knowing that the order will be processed to spec.

Essentially, special protocols enable commercial customers to automate the order process while still customizing the production process.

About Formulations

Formulation refers to the final yield, buffer, and concentration of the delivered material. These parameters determine the number of reactions an oligo, or oligo pool, will provide in an assay or experiment.

The concentration, buffer, and volume fields can be defined on the order page. Yield is determined by the starting scale, length, purification, and modifications.


Additional Information

Current Online Options for Special Protocols

The following list is subject to change. Furthermore, it does not account for all special protocols offered by our lab, but is intended to capture common requests typically placed on a reorder for bulk material.

  1. GMP: Commercialization of a product requires companies to adhere to high regulatory standards, all the way through to their suppliers. Eurofins Genomics has been approved by the FDA for cGMP manufacture of ASRs (Analyte Specific Reagents) for use in IVD products. New customers should contact us to discuss their GMP project before selecting this option on the website. GMP projects typically require several meetings between the supplier and client before production can begin, so that both parties can assess the requirements of the project and prepare. For existing customers seeking to reorder bulk material for a pre-defined project, the online option is very helpful to streamline the ordering process. Learn more about GMP.
  2. Pooling and Multiplexing: Mixing multiple sequences together is a novel requirement within the industrial sector, especially when targeting many different regions within one assay. These pools can range from a single plate up to hundreds of thousands of oligos. Learn more on pooling (tab 4 of new page)
  3. Custom Yield: This protocols ensures a guaranteed yield is met on the order.
  4. Additional Concentration Check: Additional concentration checks help to ensure the exact yield, volume, and concentration is present in the final deliverable. Commercial assays, to give one example, require highly specific concentrations and may need a second concentration check. The normal acceptable variance may not be sufficient.
  5. Stamping: Stamping involves pipetting out small amounts of a source material into multiple, secondary plates at a specific concentration.
  6. Special Normalization Requirements: this protocol is used to assign customized normalization requirements to an order.

Additional protocols are available by request, suc has custom HPLC columns, custom plate types, annealing, expedited processing, and more.

 

Special projects can be handled manually or digitally using the eCommerce platform, depending on the level of complexity, customization, and hands-on attention required. The digital options are available on the DNA oligo plate order page. This option is mainly geared for companies who have already discussed the specifications of their project with us and need to order additional material on a regular basis. In this regard, the online options offer a convenient solution to capture additional services throughout the entire process.

How to Order Online

  1. Go to the DNA plate order page.
  2. On step 2, select “yes’ beside special protocols and select which protocols you wish to add to the order.
  3. Complete the remaining steps on the order page.
  4. Go to the cart. The price will appear as “n/a” due to the inherent complexity involved in special projects. The order must be submitted as a quote for manual pricing.
  5. Click the quote button instead of checkout.
  6. Submit the quote. These steps mirror the normal checkout process You will not be charged until you decide to convert the quote into an order.
  7. The technical support team will call you to gather information on the project.
  8. Once the project scope is clear, our technical support team will complete the quote and published it back to the website.
  9. Go to the My Quotes page and click the accept button.
  10. Checkout the order.

 

Restrictions

  • Not allowed for small volume orders. Due to the cost and labor involved, these services are designed for commercial companies and custom projects, not small orders.
  • Complex projects may require a meeting with the customer to further understand their needs and the project scope.
  • If you currently enter an ISOP number in the order notes, please continue to do so. These options do not replace the ISOP.

Contact us

Not all projects can be ordered online. If your project requires a customized workflow, email our technical team to discuss the project. We may be able to set up a customized solution depending on the scope, requirements, goals, and production workflow required.

Science evolves. Companies must evolve with it. As our customers evolve to meet the demands of novel research fields such as diagnostics, NGS, CRISPR, and therapeutics, Eurofins Genomics is right beside them—innovating, expanding, and preparing new services to accelerate your product’s commercialization.

A Partner for Commercial Companies

You are searching for a partner, not just a supplier. It is key to understand that principle. You are about to embark on an exciting journey to develop an assay, kit, or commercial product which could save lives, improve healthcare, or even reshape the world. It will be exhilarating but also stressful. Each milestone will be defined by challenges. Fortunately, Eurofins Genomics is built to alleviate that stress and help you overcome challenges. We are the partner that will go the extra mile to make your assay perform to its highest level.

The commercialization process is shown below. It begins with research and ends with the commercial launch of a product. Each phase has different needs, which Eurofins Genomics is prepared to deliver on. For instance, the research phase typically involves smaller orders with experimental sequence designs. The sequences can sometimes be challenging and exciting to work on. Meanwhile, the commercial phase involves ramping up to larger amounts of material while ensuring quality never diminishes.

Industries

A wide variety of industries utilized these special protocols, but the key sectors include diagnostics (Dx), NGS, CRISPR, synthetic biology, biotech, and therapeutics.

These sectors require a higher degree of customization from suppliers due to the sensitivity of equipment, regulatory requirements, and supply chain needs. No matter the industry, Eurofins Genomics is committed to providing the highest quality and service for your products.

Types of Products

  • Research and Pre-commercial--includes RUO (research use only) and IUO (investigational use only).
  • Lab Developed Tests (LDT)--a complete In Vitro test produced in a single laboratory.
  • Companion Diagnostics (CDx)—designed to be used in conjunction with treatment by a physician.
  • Commercial Product—FDA approved product to be used with patient samples.

Categories

The range of services can vary dramatically but a majority fall into one or more of the following categories. Further detail can be found in the previous tab on ordering online.

  1. Customization to the overall production workflow, including oligo synthesis, liquid handling, quality control, and purification.
  2. Scale—commercialization involves synthesizing at higher scales, which is typically delivered in bulk lots.
  3. Purity—due to the sensitivity of NGS equipment, many commercial assays require high purification.
  4. Regulatory requirements and GMP.
  5. Pooling, annealing, and multiplexing after synthesis is completed.
  6. Integration with LIMS and/or IT systems.
  7. Formulation to reach specific concentration and volume levels.
  8. Quality control including both additional QC methods and additional QC checks.
  9. Packing and kitting


Industry Needs

NGS technology is the catalyst behind much of the explosive growth within the life science industry over the past decade. The decrease in cost and rise in the portability of DNA sequencers, combined with the development of NGS techniques, has provided high throughput analysis and sequencing of genomic data. NGS oligonucleotide components include NGS adaptors/indexes, UDIs, barcoded adaptors, targeted enrichment panels, and hybridization capture probes for targeted sequencing. These demanding NGS applications require rigorous manufacturing processes due to the ability to detect single molecules. Purity, as it pertains to oligos, is especially important to avoid adapter cross-contamination that will lead to misalignment in sequencing results

Furthermore, custom oligonucleotides are a critical component of molecular diagnostic assays. Almost any assay developed today requires oligos, specifically probes, and primers. Furthermore, companies require consistent quality between production batches since the oligos determine the performance parameters of their experiment, such as the detection limit and specificity of their assay. In summary, MDx customers need an oligonucleotide manufacturing process that is GMP compliant for their commercial products.

Scaling up

Scale up, sometimes referred to as the “ramp up” phase, refers to the supplier taking an MDx assay from small-scale RUO to large-scale manufacturing. Preserving product quality and functional performance through this period is critical. The end goal is to produce single batch sizes to the customer’s yield specifications.

The typical requirement is to have a single production batch that produces the yield requirement that typically ranges from 1.0 µmole to multiple grams.

Regulatory / QC / QA /Traceability

One of the most important criteria for a large commercial company is batch-to-batch reproducibility so their assay performs exactly the same with each lot. All aspects of our manufacturing process are monitored and documented resulting in a formal batch record. Our QA teams will support your vendor qualifications, paper audits, on-site audits, and the overall product release of GMP orders. In some cases, a certificate of analysis must be signed before the product can be released by the QA team.

Packaging and Kitting

Depending on customer’s market strategy, they may need Eurofins Genomics, the oligo supplier, to ship the material in customized packages. This process can include additional services such as kitting and distributing to the end customer as well.

 

Formulation and Normalization Pricing

Pricing for formulations and normalization are available on the price list page.

Special Protocol Pricing

Typically special protocol pricing is customized to meet the needs of the project and linked to your account so that pricing is automated on future orders. Orders are placed on the custom oligo plate order page and various options are selected under the Special Protocols section. If we have not discussed the project before, you may see "n/a" as the price in the cart. This is because pricing has not been set up yet for your project. There are two paths forward.

Option 1: you can submit it as a quote even if the price shows n/a by clicking the quote button.Our technical team will review the quote and may contact you to better understand the project. Once the scope is clear, our technical team will update the quoted price and publish it back to the website.

Option 2: contact your local sales representative, or GenomicsSupport@eurofins.com, to discuss the project. Once pricing has been agreed by both parties, we can link the pricing to your account.

Pricing can be set up as a percentage of the order cost in order to scale with the size of the order, or it can be set up as a flat rate fee, depending on the type of service and complexity of the project. Click the price details link in the cart to view the price of each item.