Modifications

Standard Oligonucleotide Modifications for custom DNA and RNA synthesis

Eurofins Genomics offers a wide variety of modifications for both DNA and RNA oligos to meet the needs of virtually any project. Modifications that are regularly stocked in-house are considered the "standard modifications portfolio." Standard modifications are listed below.

Non-standard modifications

Non-standard modifications refer to a.) custom modifications supplied by the customer, b.) special requests from customers for modifications not listed in the standard portfolio, and c.) modifications that we have prior expertise with but may not keep in stock due to the infrequency of orders. In short, any modification that is not listed in the standard portfolio. Eurofins Genomics can accommodate requests for non-standard modifications. We understand that much of science revolves around experimentation and new needs arise from new techniques.

Do not see a modification you want? Contact us to request a modification.

Specifications										Pricing
Modification / Category	Designator	5'	Int	3'	DNA or RNA	Tubes or Plates	Max Abs (nm)	Max Em (nm)	Extinction coefficient (M-1cm–1)	25 nmole	50 nmole	250 nmole	1 µmole	2 µmole	5 µmole	10 µmole	Notes
Fluorophores
Alexa Fluor 350	[Alexa350]	✓	✓	✓	DNA	T	346	424	19000	--	$653.00	$653.00	$1,686.00	$2,774.00	$6,935.00	--	Alternative to AMCA
CF 405S succinimidyl ester (CF405SN)	[CF405SN]	✓	✓	✓	DNA	T	404	431	33000	--	--	$263.00	$1180.00	--	--	--	--
Tide Fluor 1	[TF1]	✓	✓	✓	DNA	T	341	447		--	$140.00	$140.00	$248.00	$320.00	$800.00	$1,372.00
ATTO 390	[ATTO390]	✓	✓	✓	DNA	T	390	479	24000	--	$152.00	$221.00	$305.00	$551.00	$1,448.75	--
5-FAM	[5FAM]	✓			Both	T	492	518		--	$55.00	$70.00	$145.00	$158.00	$230.00	$525.00	Alternative to CY2, DY-495, ATTO 495
Alexa Fluor 488	[Alexa488]	✓	✓	✓	DNA	T	496	519	71000	--	$300.00	$300.00	$450.00	$509.00	$778.05	--
Fluorescein	[FL]	✓	✓		DNA	T	495	520	83000	--	$65.00	$81.00	$175.00	$248.00	$452.00	--	Alternative to DY-495, ATTO 495
6-FAM	[FAM]	✓		✓	Both	Both	495	521	75000	--	$68.00	$85.00	$184.00	$239.00	$342.00	$525.00
Fluorescein dT	[FLUdT]	✓	✓		DNA	T	494	522	75000	--	$200.00	$225.00	$320.00	$359.10	$685.90	$1,600.00	Alternative to DY-495, ATTO 495
Tide Fluor 2	[TF2]	✓	✓	✓	DNA	T	499	522		--	$140.00	$140.00	$248.00	$320.00	$800.00	$929.00
ATTO 488	[ATTO488]	✓	✓	✓	DNA	T	501	523	90000	--	$205.00	$273.00	$378.00	$467.40	$718.20	--	Alternative to Cy2
Oregon Green 488	[OG488]	✓	✓	✓	DNA	T	496	524	76000	--	$375.00	$375.00	$565.00	$638.40	$978.50	$5,750.00	Alternative to Cy2
TET	[TET]	✓			Both	Both	519	539	86000	--	$75.00	$94.00	$190.00	$380.00	$950.00	$750.00	Alternative to CAL Fluor Gold 540
CAL Fluor Gold 540	[CalFluor540]	✓			DNA	T			522		--	--	--	--	--	--	Alternative to 81100
JOE	[JOE]	✓	✓	✓	DNA	T	520	548	73000	--	$205.00	$205.00	$310.00			$7,400.00	Alternative to CAL Fluor Gold 540, Rhodamine 6G
Yakima Yellow	[YAKYE]	✓			DNA	T	530	549		--	$79.00	$100.00	$201.40	$265.00	$530.00	$1,060.00	Alternative to VIC, CAL Fluor 560
Alexa Fluor 532	[Alexa532]	✓	✓	✓	DNA	T	532	553	81000	--	$375.00	$375.00	$565.00	$638.40	$978.50	$5,750.00
ATTO 532	[ATTO532]	✓	✓	✓	DNA	T	532	553	115000	--	$205.00	$273.00	$378.00	$469.30	$718.20	--
HEX	[HEX]	✓			Both	Both	537	556	96000	--	$75.00	$94.00	$190.00	$214.70	$324.90	$683.00	Alternative to VIC, CAL Fluor 560, SIMA
Alexa Fluor 555	[Alexa555]	✓	✓	✓	DNA	T	555	565	150000	--	$653.00	$653.00	$1,686.00	$2,774.00	$6,935.00	--	Alternative to Cy3, TRITC & TMR, Alexa Fluor 546
Alexa Fluor 546	[Alexa546]	✓	✓	✓	DNA	T	556	573	104000	--	$375.00	$375.00	$565.00	$633.65	$978.50	$5,750.00
ATTO 550	[ATTO550]	✓	✓	✓	DNA	T	554	576	120000	--	$205.00	$273.00	$378.00	$467.40	$718.20	--	Alternative to NED, Cy3, Rhodamine B & 6G
TAMRA dT	[TAMdT]	✓	✓		DNA	T	544	576	90000	--	$156.00	$283.00	$422.00	$693.50	$1,733.75	--
Tide Fluor 3	[TF3]	✓	✓	✓	DNA	T	554	578		--	$140.00	$140.00	$248.00	$320.00	$800.00	$929.00
TAMRA	[TAM]			✓	DNA	Both	556	580	89000	--	$98.00	$115.00	$175.00	$194.75	$299.25	$780.00	Alternative to Rhodamine 6G, Rhodamine B, CAL Fluor 590
5-Tamra (ester)	[TMR5]	✓			DNA	T	557	583		--	$130.00	$155.00	$250.00	$279.30	$738.15	$1,700.00
CAL Fluor 590	[CalFluor590]	✓			DNA	T	569	591	79000	--	350.00	$350.00	$455.00	$865.50	$2,161.25	$3,640.00
ATTO 565	[ATTO565]	✓	✓	✓	DNA	T	563	592	120000	--	$174.00	$263.00	$352.00	$435.10	$668.80	--	Alternative to PET, Alexa Fluor 594
ROX	[ROX]	✓	✓	✓	DNA	T	575	602	82000	--	$174.00	$174.00	$263.00	$324.90	$498.75	$1,790.00	Alternative to Texas Red, Alexa Fluor 594, CAL Fluor Red 610
Alexa Fluor 568	[Alexa568]	✓	✓	✓	DNA	T	578	603	91000	--	$653.00	$653.00	$1,686.00	$2,774.00	$6,935.00	--
Texas Red	[TxRed]	✓	✓	✓	DNA	T	583	603	116000	--	$200.00	$242.00	$347.00	$428.25	$658.35	--	Alternative to ROX, Alexa Fluor 594
Cyanine3.5 dye	[Cy35]	✓	✓		DNA	T	591	604	116000	--	$173.00	$185.00	$294.00	$532.50	$1,330.00	--	Alternative to Alexa Fluor 594, CAL Fluor Red 610
CAL Fluor Red 610	[CalFluor610]	✓			DNA	T	590	610	108000	--	$350.00	$350.00	$455.00	$865.50	$2,161.25	$3,640.00	Alternative to Texas Red, ROX, Alexa Fluor® 594
Tide Fluor 4	[TF4]	✓	✓	✓	DNA	T	588	610		--	$140.00	$140.00	$248.00	$320.00	$800.00	$929.00
Alexa Fluor 594	[Alexa594]	✓	✓	✓	DNA	T	590	617	73000	--	$440.00	$440.00	$670.00	$753.35	$2,498.00	$6,450.00	Alternative to ATTO 594, CAL Fluor Red 610
ATTO 590	[ATTO590]	✓	✓	✓	DNA	T	594	624	120000	--	$174.00	$263.00	$352.00	$434.15	$668.80	--
Alexa Fluor 633	[Alexa633]	✓	✓	✓	DNA	T	632	647	239000	--	$653.00	$653.00	$1,686.00	$2,774.00	$6,935.00	--
ATTO 633	[ATTO633]	✓	✓	✓	DNA	T	629	657	130000	--	$205.00	$273.00	$378.00	$467.40	$718.20	--	Alternative to LIZ, Alexa Fluor 633
Cyanine5 dye	[Cy5]	✓			Both	T	646	662	250000	--	$107.00	$135.00	$210.00	$233.70	$489.25	$1,300.00	Alternative to Quasar 670, TYE 655, TYE 665
Alexa Fluor 647	[Alexa647]	✓	✓	✓	DNA	T	650	665	239000	--	$300.00	$300.00	$450.00	$504.45	$778.05	--	Alternative to Cy5, Quasar 670
ATTO 647N	[ATTO647N]	✓	✓	✓	DNA	T	644	669	150000	--	$205.00	$273.00	$378.00	$467.40	$718.20	--	Alternative to Cy5, Quasar 670
Tide Fluor 5	[TF5]	✓	✓	✓	DNA	T	656	670		--	$140.00	$140.00	$248.00	$320.00	$800.00	$929.00
ATTO 655	[ATTO655]	✓	✓	✓	DNA	T	663	684	125000	--	$152.00	$221.00	$305.00	$579.50	$1,448.75	--
Alexa Fluor 660	[Alexa660]	✓	✓	✓	DNA	T	663	690	132000	--	$440.00	$440.00	$670.00	$753.35	$1,154.25	$6,450.00
Alexa Fluor 680	[Alexa680]	✓	✓	✓	DNA	T	679	702	184000	--	$653.00	$653.00	$1,686.00	$2,774.00	$6,935.00	--	Alternative to Alexa Fluor 680, Alexa Fluor 700
Tide Fluor 6	[TF6]	✓	✓	✓	DNA	T	686	702		--	$140.00	$140.00	$248.00	$320.00	$800.00	$929.00
Cyanine5.5 dye	[Cy55]	✓			DNA	T	688	707	209000	--	$184.00	$205.00	$310.00	$294.50	$589.00	$3,100.00	Alternative to Alexa Fluor 680, TYE 705
ATTO 700	[ATTO700]	✓	✓	✓	DNA	T	700	719	120000	--	$152.00	$221.00	$305.00	$579.50	$1448.75	--
Alexa Fluor 700	[Alexa700]	✓	✓	✓	DNA	T	702	723	192000	--	$653.00	$653.00	$1,686.00	$2,774.00	$6,935.00	--	Alternative to IRDye 700, Quasar 705
ATTO 740	[ATTO740]	✓	✓	✓	DNA	T	740	764	120000	--	$145.00	$210.00	$290.00	--	--	--	Alternative to Cy7, Alexa Fluor 740
Alexa Fluor 750	[Alexa750]	✓	✓	✓	DNA	T	749	775	240000	--	$440.00	$440.00	$670.00	$753.35	$1154.25	--
Tide Fluor 7	[TF7]	✓	✓	✓	DNA	T	756	775		--	$140.00	$140.00	$248.00	$320.00	$800.00	$929.00
Tide Fluor 8	[TF8]	✓	✓	✓	DNA	T	787	808		--	$140.00	$140.00	$248.00	$320.00	$800.00	$929.00
Quenchers
BBQ650	[BBQ650]	✓		✓	DNA	T	650	n/a	33000		$257.25	$257.25	$294.00	$588.00	$1470.00
BHQ1	[BHQ1]	✓		✓	DNA	T	534	n/a	34000	--	$110.00	$145.00	$210.00	$359.10	$1005.00	$2000.00
BHQ1 dT	[BHQ1dT]	✓	✓		DNA	T	534	n/a	34000	--	--	$163.00	$252.00	$480.00	$1,200.00	--
BHQ2	[BHQ2]	✓		✓	DNA	T	579	n/a	91000	--	$110.00	$145.00	$210.00	$270.75	$359.10	$2000.00
BHQ2 dT	[BHQ2dT]	✓	✓		DNA	T	579	n/a	91000	--	--	$278.00	$383.00	$693.50	$1,733.75	--
Dabcyl STANDARD	[DAB}			✓	DNA	T	453	n/a	32000		$92.00	$115.00	$175.00	$194.75	$374.30	$865.00
Dabcyl dT	[DABdT]	✓	✓		DNA	T	478	n/a	32000	--	$285.00	$382.50	$382.50	$690.00	$1,725.00	--
TAMRA	[TAM]	✓		✓	DNA	T	557	n/a	91000	--	$98.00	$115.00	$175.00	$194.75	$299.25	$780.00
Tide Quencher 1	[TQ1]			✓	DNA	T	490	n/a	20000	--	$46.00	$58.00	$116.00	$200.00	$375.00	$635.00
Tide Quencher 2	[TQ2]			✓	DNA	T	515	n/a	21000	--	$46.00	$58.00	$116.00	$200.00	$375.00	$635.00
Tide Quencher 3	[TQ3]			✓	DNA	T	570	n/a	22000	--	$46.00	$58.00	$116.00	$200.00	$375.00	$635.00
Tide Quencher 5	[TQ5]			✓	DNA	T	670	n/a	24000	--	$46.00	$58.00	$116.00	$200.00	$375.00	$635.00

Specifications							Pricing
Modification / Category	Designator	5'	Int	3'	DNA or RNA	Tubes or Plates	25 nmole	50 nmole	250 nmole	1 µmole	2 µmole	5 µmole	10 µmole	Notes
Mixed/Degenerate Bases (Wobbles)
N (A+C+G+T) Wobble	N	✓	✓	✓	Both	Both	$0.38	$0.50	$1.26	$2.57			$17.00	N = (A, 25%; C, 25%; G, 25%; T, 25%)
B (C+G+T) Wobble	B	✓	✓	✓	Both	Both	$0.38	$0.50	$1.27	$2.58			$17.01	B = (C, 33.3%; G, 33.3%; T, 33.3%)
D (A+G+T) Wobble	D	✓	✓	✓	Both	Both	$0.38	$0.50	$1.28	$2.59			$17.02	D = (A-33.3%, G-33.3%, T-33.3%)
H (A+C+T) Wobble	H	✓	✓	✓	Both	Both	$0.38	$0.50	$1.29	$2.60			$17.03	H = (A, 33.3%; C, 33.3%; T, 33.3%)
V (A+C+G) Wobble	V	✓	✓	✓	Both	Both	$0.38	$0.50	$1.30	$2.61			$17.04	V = (A, 33.3%; C, 33.3%; G, 33.3%)
K (G+T) Wobble	K	✓	✓	✓	Both	Both	$0.38	$0.50	$1.31	$2.62			$17.05	K = (G, 50%; T, 50%)
M (A+C) Wobble	M	✓	✓	✓	Both	Both	$0.38	$0.50	$1.32	$2.63			$17.06	M = (A, 50%; C, 50%)
R (A+G)(purines) Wobble	R	✓	✓	✓	Both	Both	$0.38	$0.50	$1.33	$2.64			$17.07	R = (A, 50%; G, 50%)
S (C+G)(strong bonds) Wobble	S	✓	✓	✓	Both	Both	$0.38	$0.50	$1.34	$2.65			$17.08	S = (C, 50%; G, 50%)
W (A+T)(weak bonds) Wobble	W	✓	✓	✓	Both	Both	$0.38	$0.50	$1.35	$2.66			$17.09	W = (A, 50%; T, 50%)
Y (C+T)(pyrimidines) Wobble	Y	✓	✓	✓	Both	Both	$0.38	$0.50	$1.36	$2.67			$17.10	Y = (C, 50%; T, 50%)
Wobble, Hand-Mixed	[%-%-%-%]	✓	✓	✓	Both	Both		$8.00	$8.00	$10.00	$20.00	$50.00	$95.00	User defined
Attachment Modifications
Amino Modifier C12	[AmC12]	✓			DNA	Both	$63.00	$63.00	$81.00	$120.00	$136.80	$209.95	$485.00
Amino Modifier C3	[AmC3]	✓		✓	DNA	T	--	$105.00	$105.00	$147.00	$266.00	$665.00	--
Amino Modifier C6	[AmC6]	✓			Both	Both	$15.00	$28.00	$37.00	$63.00	$70.30	$190.00	$230.00
Amino Modifier C6-dT	[AmC6dT]	✓	✓		Both	Both	--	$131.00	$158.00	$237.00	$292.60	$449.35	$945.00
Amino Modifier C7	[AmC7]		✓	✓	Both	Both	--	$25.00	$25.00	$40.00	$76.00	$190.00	$250.00
Uni-Link AminoModifier	[AmUni]	✓	✓		DNA	Both	--	$79.00	$100.00	$152.00	$190.00	$289.75	$609.00
i3Thiodl	[i3Thiodl]	✓	✓		DNA	T	--	--	$263.00	$1180.00			--
Thiol Modifier C6 S-S	[ThiolC6]	✓	✓		DNA	T	--	$145.00	$145.00	$225.00	$450.00	$1,125.00	$865.00
Thiol Mod C3 S-S	[ThiolC3]			✓	DNA	T	--	$115.00	$115.00	$175.00	$450.00	$1,125.00	$865.00
Phosphate	[PHO]	✓		✓	Both	Both	$23.00	$23.00	$29.00	$52.00	$58.90	$89.30	$210.50
Non-flourescent labels
Biotin	[BIO]	✓		✓	Both	Both	--	$53.00	$63.00	$121.00	$149.15	$219.45	$441.00
Biotin PC (Photocleavable)	[BioPC]	✓			DNA	T		$152.00	$152.00	$289.00	--	--	$1,440.00
Biotin-dT	[BIOdT]	✓	✓		DNA	Both	--	$200.00	$200.00	$320.00	$342.00	$653.13	$1,600.00
Biotin-ON	[BioON]	✓	✓		Both	T	--	$58.00	$74.00	$121.00	$218.50	$546.25	$604.00
Biotin-TEG STD (5' or 3')	[BIOTEG]	✓		✓	Both	Both		$92.00	$115.00	$150.00	$169.10	$299.25	$695.00
Biotin-TEG (internal)	[BIOTEGi]		✓		Both	Both	--	$53.00	$53.00	$105.00	$190.00	$475.00	$525.00
Digoxigenin (ester)	[DIG]	✓	✓	✓	DNA	T	--	$135.00	$135.00	$200.00			$1,627.50
DNP (2,4-dinitrophenyl)	[DNP]	✓	✓		DNA	T	--	$20.00	$25.00	$45.00			$180.00
Modified Bases, Spacers, Miscellaneous
23ddC	[23ddC]			✓	DNA	T		$90.00	$90.00	$180.00			$920.00
5-Methyl dC	[5MedC]	✓	✓	✓	DNA	T	--	$42.00	$52.50	$78.75			$590.50
Acrydite	[5Acrd]	✓			DNA	T	--	$84.00	$105.00	$158.00	$316.00	$475.00	$630.00
Cholesteryl-TEG	[CholTEG]	✓	✓		Both	T	--	$92.00	$92.00	$275.00	$550.00	$1,375.00	$1,400.00
Cholesteryl	[Chol]			✓	DNA	T	--	$92.00	$92.00	$275.00	$550.00	$1,375.00	$1,400.00
5' Dabcyl-dT	[5DabsyldT] or [5DabcyldT]	✓	✓		DNA	T	--	--	$155.00	$262.50			--
deoxyInosine [I]	[I]	✓	✓		Both	Both	$10.50	$10.50	$12.60	$23.00	$37.91	$50.35	$94.00
deoxyUridine [U]	[U]	✓	✓		Both	Both	$10.50	$10.50	$12.60	$23.00	$37.05	$50.35	$94.00
dP + dK mixture	[dPdK]	✓	✓		DNA	T	--	$194.00	$194.00	$310.00			$1,851.00
dT-5' (reversed base)	[dTrev]			✓	DNA	T		$56.18	$67.00	$102.82	$130.00	$225.00	$420.00
pdC	[pdC]	✓	✓		DNA	T	$10.50	$10.50	$14.50	$23.00			$94.00
pdU	[pdU]	✓	✓		DNA	T	$10.50	$10.50	$14.50	$23.00			$94.00
Phosphorothioation (PTO Bond)	*		✓		DNA	T	--	$3.50	$3.50	$5.00	$12.00	$24.00	$35.00
dSpacer	[Spd]	✓	✓		DNA	T	--	$89.00	$105.00	$158.00	$194.75	$299.25	$630.00
Spacer C18	[SP18]	✓	✓		DNA	T	--	$48.00	$58.00	$94.00	$116.85	$179.55	$378.00
Spacer C3	[SpC3]	✓	✓	✓	DNA	Both	--	$52.00	$63.00	$98.00	$110.20	$150.10	$345.00
Spacer C9	[SpC9]	✓	✓		DNA	T	--	$65.00	$69.00	$98.00	--	--	$345.00
TINA	[TINA]	✓			DNA	T	--	$52.00	$64.00	$110.00	$220.00	$450.00	--
2'-Fluoro-A	[2FlA]	✓	✓		RNA	T	$22.00	$41.25	$41.25	$62.70			$319.00
2'-Fluoro-C	[2FlC]	✓	✓		RNA	T	$22.00	$41.25	$41.25	$62.70			$319.00
2'-Fluoro-G	[2FlG]	✓	✓		RNA	T	$22.00	$41.25	$41.25	$62.70			$319.00
2'-Fluoro-U	[2FlU]	✓	✓		RNA	T	$22.00	$41.25	$41.25	$62.70			$319.00
2'-O-Methyl A	[2OMeA]	✓	✓	✓	RNA	T		$9.45	$13.65	$21.00	$42.00	$78.00	$163.00
2'-O-Methyl C	[2OMeC]	✓	✓	✓	RNA	T		$9.45	$13.65	$21.00	$42.00	$78.00	$163.00
2'-O-Methyl G	[2OMeG]	✓	✓	✓	RNA	T		$9.45	$13.65	$21.00	$42.00	$78.00	$163.00
2'-O-Methyl U	[2OMeU]	✓	✓	✓	RNA	T		$9.45	$13.65	$21.00	$42.00	$78.00	$163.00
2-MethoxyEthoxy A	[2MoeA]	✓	✓	✓	RNA	T	--	$20.00	$26.00	$46.00	$58.00	--	--
2-MethoxyEthoxy MeC	[2MoeC]	✓	✓	✓	RNA	T	--	$20.00	$26.00	$46.00	$58.00	--	--
2-MethoxyEthoxy G	[2MoeG]	✓	✓	✓	RNA	T	--	$20.00	$26.00	$46.00	$58.00	--	--
2-MethoxyEthoxy T	[2MoeT]	✓	✓	✓	RNA	T	--	$20.00	$26.00	$46.00	$58.00	--	--
Click Chemistry
Azide	[Azide]	✓	✓	✓	DNA	T	--	$110.00	$140.00	$200.00			$1,400.00
Hexynyl	[Hexynyl]	✓	✓	✓	DNA	T	--	$118.00	$147.00	$224.00			$890.00

Regulated Environments

Our oligos are manufactured in our ISO 9001:2008 and ISO 13485:2003 certified facility in Louisville, Alabama. Eurofins Genomics quality management system is assurance that our oligos and other products and services are suitable for use in your cGMP, ASR, or QS regulation projects. Contact us at 1-800-688-2248 to discuss high throughput modified oligos for your FDA or CLIA regulated projects.

Eurofins Genomics offers many more modifications in addition to the standard portfolio, which is covered in the previous tabs. Non-standard modifications are amidites that we are experienced with using but are not regularly stocked in our inventory for cost reasons. Below is a list of common, non-standard modifications. If you do not see the modification that you are looking for, contact us! Our chemists are always up for a challenge.

Please note that these modifications may not be visible on the order pages. To request one, email GenomicsSupport@eurofins.com.

Attachment Mods

Amino, thiol, acrydite, and phosphate groups incorporated during synthesis allow post synthesis reactions for attaching a variety of labels or for coupling the oligo with other molecules.

Modifier [Legacy Designator]	Availability	Structure
3'-PT-Amino-Modifier C3 [AmC3-PT]	3'
Amino Modifier C12 [AmC12]	5'
Amino Modifier C2 dT [AmC2-dT]	5', Internal
Amino Modifier C3 [AmC3]	5'
Amino Modifier C6 [AminoC6]	5'
Amino Modifier C6 dT [AmC6-dT]	5', Internal
Amino Modifier C7 [AmC7]	3'
AminoC6 dC [AmC6-dC]	5', Internal	Image not available.
Phosphate [Phos]	5'
Phosphate [Phos]	3'
Thiol Mod C3 S-S [ThiolC3]	3'
Thiol Modifier C6 S-S [ThiolC6]	5', Internal
Uni-Link AminoModifier [Am-Uni]	5', Internal

DNA Analogs

Molecules with similar structure to DNA often readily substitute in the DNA strand for a variety of experimental uses. Modifications in this category include inosine, uridine, wobbles, etc.

Modifier [Legacy Designator]	Availability	Structure
3-Nitropyrrole [NitPyr]	5', Internal
5-Nitroindole [5-NitIdl]	3'
5-Nitroindole [5-NitIdl]	5', Internal
AP-dC [AP-dC]	5', Internal	Image not available.
dK [dK]	5', Internal
dP [dP]	3'
dP [dP]	5', Internal
dP + dK mixture [dPdK]	5', Internal
deoxyInosine [I]	5', Internal
deoxyInosine [I]	3'
deoxyUridine [U]	5', Internal
deoxyUridine [U]	3'

Fluorophores

Fluorescent molecules offer a wide variety of applications requiring detection of hybridization including in situ probes, sequencing, and genotyping.

Modifier [Legacy Designator]	Availability	Structure
5-FAM [5-fam]	5'
6-Carboxyfluorescein [6Fl]	5', Internal
6-Carboxyrhodamine (ester) [CR6G]	5', 3', Internal
6-FAM [6-Fam]	3'
6-FAM (standard) [6-FAM]	5'
6-Joe, SE (ester) [JOE]	5', 3', Internal
ATTO390 (ester) [ATTO390]	5', 3', Internal	Image not available.
ATTO488 (ester) [ATTO488]	5', 3', Internal	Image not available.
ATTO532 (ester) [ATTO532]	5', 3', Internal	Image not available.
ATTO550 (ester) [ATTO550]	5', 3', Internal	Image not available.
ATTO565 (ester) [ATTO565]	5', 3', Internal	Image not available.
ATTO590 (ester) [ATTO590]	5', 3', Internal	Image not available.
ATTO633 (ester) [ATTO633]	5', 3', Internal	Image not available.
ATTO647N (ester) [ATTO647N]	5', 3', Internal	Image not available.
ATTO655 (ester) [ATTO655]	5', 3', Internal	Image not available.
ATTO700 (ester) [ATTO700]	5', 3', Internal	Image not available.
ATTO740 (ester) [ATTO740]	5', 3', Internal	Image not available.
Alexa Fluor 350 (ester) [Alexa350]	5', 3', Internal
Alexa Fluor 488 (ester) [Alexa488]	5', 3', Internal
Alexa Fluor 532 (ester) [Alexa532]	5', 3', Internal
Alexa Fluor 546 (ester) [Alexa546]	5', 3', Internal
Alexa Fluor 555 (ester) [Alexa555]	5', 3', Internal	Image not available.
Alexa Fluor 568 (ester) [Alexa568]	5', 3', Internal
Alexa Fluor 594 (ester) [Alexa594]	5', 3', Internal
Alexa Fluor 633 (ester) [Alexa633]	5', 3', Internal	Image not available.
Alexa Fluor 647 (ester) [Alexa647]	5', 3', Internal	Image not available.
Alexa Fluor 660 (ester) [Alexa660]	5', 3', Internal	Image not available.
Alexa Fluor 680 (ester) [Alexa680]	5', 3', Internal	Image not available.
Alexa Fluor 700 (ester) [Alexa700]	5', 3', Internal	Image not available.
Alexa Fluor 750 (ester) [Alexa750]	5', 3', Internal	Image not available.
Cal Fluor Red 610	5'	Image not available.
Cyanine3 [Cyanine3]	5'
Cyanine3 (ester) [Cyanine3es]	3'	Image not available.
Cyanine3.5 [Cyanine35]	5', Internal
Cyanine3.5 (ester) [Cyanine35es]	3'	Image not available.
Cyanine5 [Cyanine5]	5'
Cyanine5 (ester) [Cyanine5es]	3'	Image not available.
Cyanine5.5 [Cyanine55]	5'
DY682 (replaces IRD700) [DY682]	5', 3'	Image not available.
DY782 (replaces IRD800) [DY782]	5'	Image not available.
Fluorescein [Fl]	5', Internal
Fluorescein-dT [Fl-dT]	5', Internal
HEX [5HEX]	5'
Oregon Green 488 (ester) [OrGre]	5', 3', Internal
ROX (ester) [ROX]	5', 3', Internal
TAMRA (STANDARD) [TAMRA]	5'
TAMRA (STANDARD) [Tamra]	3'
TAMRA-dT [TAMdT]	5', Internal
TET [5TET]	5'
Tamra (ester) - 5-isomer [TMR-5]	5', 3', Internal
Texas Red-X (ester) [TxRed]	5', 3', Internal
Tide Fluor 1 [TF1]	5', 3', Internal	Image not available.
Tide Fluor 2 [TF2]	5', 3', Internal	Image not available.
Tide Fluor 3 [TF3]	5', 3', Internal	Image not available.
Tide Fluor 4 [TF4]	5', 3', Internal	Image not available.
Tide Fluor 5 [TF5]	5', 3', Internal	Image not available.
Tide Quencher 2 ester [TQ2es]	5', 3', Internal	Image not available.

Mixed Bases

Standard bases used in combination to create a base comprised of a mixture of 2-4 bases (e.g. A/C or A/C/G). This category includes IUB Wobbles and custom wobbles.

Modifier [Legacy Designator]	Availability	Structure
3' B Wobble (C+G+T) [B]	3'
3' D Wobble (A+G+T) [D]	3'
3' H Wobble (A+C+T) [H]	3'
3' K Wobble (G+T) [K]	3'
3' M Wobble (A+C) [M]	3'
3' N Wobble (A+C+G+T) [N]	3'
3' R Wobble (A+G) [R]	3'
3' S Wobble (C+G) [S]	3'
3' V Wobble (A+C+G) [V]	3'
3' W Wobble (A+T) [W]	3'
3' Y Wobble (C+T) [Y]	3'
B Wobble (C+G+T) [B]	5', Internal
D Wobble (A+G+T) [D]	5', Internal
H Wobble (A+C+T) [H]	5', Internal
K Wobble (G+T) [K]	5', Internal
M Wobble (A+C) [M]	5', Internal
Mix-1 MG [Mix-1]	5', Internal	Image not available.
N Wobble (A+C+G+T) [N]	5', Internal
R Wobble (A+G) [R]	5', Internal
S Wobble (C+G) [S]	5', Internal
V Wobble (A+C+G) [V]	5', Internal
W Wobble (A+T) [W]	5', Internal
Wobble 10%A 20%G 70%T [10-00-20-70]	5', 3', Internal
Wobble 20%A 20%C 60%G [20-20-60-00]	5', 3', Internal
Wobble 20%A 20%C 60%T [20-20-00-60]	5', 3', Internal
Wobble 20%A 40%C 40%T [20-40-00-40]	5', 3', Internal
Wobble 20%A 80%G [20-0-80-0]	5', 3', Internal
Wobble 30%A 30%C 40%T [30-30-00-40]	5', 3', Internal
Wobble 30%A 70%G [30-0-70-0]	5', 3', Internal
Wobble 40%A 30%G 30%T [40-00-30-30]	5', 3', Internal
Wobble 40%A 40%G 20%T [40-00-40-20]	5', 3', Internal
Wobble 60%A 20%C 20%G [60-20-20-00]	5', 3', Internal
Wobble 60%A 20%G 20%T [60-00-20-20]	5', 3', Internal
Wobble 60%C 20%G 20%T [00-60-20-20]	5', 3', Internal
Wobble 70%A 20%C 10%T [70-20-00-10]	5', 3', Internal
Wobble 70%C 30%T [0-70-0-30]	5', 3', Internal
Wobble 80%A 20%C [80-20-00-00]	5', 3', Internal
Wobble 80%C 20%G [00-80-20-00]	5', 3', Internal
Wobble 80%C 20%T [00-80-00-20]	5', 3', Internal
Wobble 90%C 10%T [00-90-00-10]	5', 3', Internal
Y Wobble (C+T) [Y]	5', Internal

Quenchers

Quenchers are most commonly paired with specific fluorophores and are chosen based on their ability to absorb energy at the emission wavelength of the fluorophore.

Modifier [Legacy Designator]	Availability	Structure
5-Dabcyl [5-Dabcyl]	5'
BHQ1-dT [BHQ1-dT]	5', Internal
BHQ2-dT [BHQ2-dT]	5', Internal
Black Hole Quencher 1 [BHQ1]	5', Internal
Black Hole Quencher 1 [BHQ1a]	3'
Black Hole Quencher 2 [BHQ2]	5', Internal
Black Hole Quencher 2 [BHQ2a]	3'
Dabcyl (STANDARD) [Dabcyl]	3'
Dabsyl-dT [Dab-dT]	5', Internal
QSY 7 (ester) [QSY7]	5', 3', Internal
Tide Quencher 1 [TQ1]	3'	Image not available.
Tide Quencher 2 [TQ2]	3'	Image not available.
Tide Quencher 3 [TQ3]	3'	Image not available.
Tide Quencher 4 [TQ4]	3'	Image not available.

Spacers

Spacers form a non-binding bridge between areas of an oligo. Among our offerings are C3 spacer which can substitute for an unknown base and Spacer 18 which introduces a hydrophobic area in an oligo.

Modifier [Legacy Designator]	Availability	Structure
Spacer 18 [Sp-C18]	5', Internal
Spacer 9 [Sp-9]	5', Internal
Spacer C3 [Sp-3]	3'
Spacer C3 [Sp-3]	5', Internal
dSpacer [Sp-d]	5', Internal

Biotins

Biotin offers a variety of uses in research including assays in drug discovery, histological, and cytological applications and capture methods are based on the high affinity of biotin for avidin and streptavidin.

Modifier [Legacy Designator]	Availability	Structure
3'-Biotin [Biotin]	3'	Image not available.
5'-Biotin [Biotin-5]	5'
Biotin PC (Photocleavable) [Bio-PC]	5'
Biotin-ON [Bio-ON]	5', Internal
Biotin-TEG (STANDARD) [BioTEG]	3'
Biotin-TEG (STANDARD) [BioTEG]	5'
Biotin-TEG (internal) [BioTEGi]	Internal
Biotin-dT [Bio-dT]	5', Internal

Dual-labeled Probes

A selected grouping of highly popular Fluorophore/Quencher pairings for real-time/qPCR applications.

Modifier [Legacy Designator]	Availability	Structure
3' BHQ 1 + 5' 6-FAM [BHQ1a-6FAM]	5', 3'	Image not available.
3' BHQ 1 + 5' HEX [BHQ1a-5HEX]	5', 3'	Image not available.
3' BHQ 1 + 5' JOE [BHQ1a-JOE]	5', 3'	Image not available.
3' BHQ 1 + 5' Oregon Green [BHQ1a-OrGre]	5', 3'	Image not available.
3' BHQ 1 + 5' TET [BHQ1a-5TET]	5', 3'	Image not available.
3' BHQ 2 + 5' Cyanine3 [BHQ2a-Cyanine3]	5', 3'	Image not available.
3' BHQ 2 + 5' Cyanine3.5 [BHQ2a-Cyanine35]	5', 3'	Image not available.
3' BHQ 2 + 5' Cyanine5 [BHQ2a-Cyanine5]	5', 3'	Image not available.
3' BHQ 2 + 5' Cyanine5.5 [BHQ2a-Cyanine55]	5', 3'	Image not available.
3' BHQ 2 + 5' HEX [BHQ2a-5HEX]	5', 3'	Image not available.
3' BHQ 2 + 5' ROX [BHQ2a-ROX]	5', 3'	Image not available.
3' BHQ 2 + 5' TAMRA [BHQ2a-TAMRA]	5', 3'	Image not available.
3' BHQ 2 + 5' Texas Red [BHQ2a-TxRed]	5', 3'	Image not available.
3' TQ1 + 5' TF1 [TQ1-TF1]	5', 3'	Image not available.
3' TQ2 + 5' TF2 [TQ2-TF2]	5', 3'	Image not available.
3' TQ3 + 5' TF3 [TQ3-TF3]	5', 3'	Image not available.
3' TQ4 + 5' TF4 [TQ4-TF4]	5', 3'	Image not available.
3' TQ5 + 5' TF5 [TQ5-TF5]	5', 3'	Image not available.
3' Tamra + 5' 6-FAM [TAMRA-6-FAM]	5', 3'	Image not available.
3' Tamra + 5' HEX [TAMRA-5HEX]	5', 3'	Image not available.
3' Tamra + 5' TET [TAMRA-5TET]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' 6-FAM [MB-6-FAM]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' CR6G [MB-56CR6G]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' Cyanine3 [MB-Cyanine3]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' Cyanine5 [MB-Cyanine5]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' HEX [MB-5HEX]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' Oregon Green [MB-OrGre]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' ROX [MB-ROX]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' TAMRA [MB-TAMRA]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' TET [MB-5TET]	5', 3'	Image not available.
Mol. Beacon, 3' Dabcyl + 5' Texas Red [MB-TxRed]	5', 3'	Image not available.

Modified Bases

Chemically modified bases can be used for structural studies and enhanced binding. This category includes Br-dU, 5-methyl dC, 5-iodo dU, 2-aminopurine, etc.

Modifier [Legacy Designator]	Availability	Structure
2-Aminopurine [2AmPur]	5', Internal
5-Bromo dU [5Br-dU]	3'
5-Bromo-dC [5Br-dC]	5', Internal
5-Bromo-dU [5Br-dU]	5', Internal
5-Iodo-dU [5I-dU]	5', Internal
5-Methyl dC [5Me-dC]	5', Internal
5-Methyl-dC [5Me-dC]	3'
O6-phenyl-dI [6phnl-dI]	5', Internal
Pyrrolo-dC [Pyrlo-dC]	5', Internal

RNA Analogs

RNA analogs are useful for creating the chimeric DNA/RNA oligos commonly used in anti-sense studies.

Modifier [Designator]	Availability	Structure
2'-Fluoro-A [2F-A]	5', Internal	Image not available.
2'-Fluoro-C [2F-C]	5', Internal	Image not available.
2'-Fluoro-G [2F-G]	5', Internal	Image not available.
2'-Fluoro-U [2F-U]	5', Internal	Image not available.
2'-O Methyl-A [2OMeA]	3'
2'-O Methyl-C [2OMeC]	3'
2'-O Methyl-G [2OMeG]	3'
2'-O Methyl-U [2OMeU]	3'
2'-O-Methyl-A [2OMeA]	5', Internal
2'-O-Methyl-C [2OMeC]	5', Internal
2'-O-Methyl-G [2OMeG]	5', Internal
2'-O-Methyl-U [2OMeU]	5', Internal
5'OH-Methyl-C [5HOMedC]	5', Internal	Image not available.
Pyrrolo-RNA-C [Pyrrolo-rC]	5', Internal	Image not available.
Ribo A [rA]	5', Internal	Image not available.
Ribo A [rA]	3'	Image not available.
Ribo C [rC]	3'	Image not available.
Ribo C [rC]	5', Internal	Image not available.
Ribo G [rG]	3'	Image not available.
Ribo G [rG]	5', Internal	Image not available.
Ribo U [rU]	5', Internal	Image not available.
Ribo U [rU]	3'	Image not available.
Ribo-8OxrG [8Ox-rG]	5', Internal	Image not available.

Miscellaneous

Miscellaneous modifications include phosphorothioate backbone modification, digoxigenin labeling, modified bases to prevent extension of the 3' terminus, cholesterol for cell delivery, puromycin (a tRNA mimc), etc.

Modifier [Designator]	Availability	Structure
2'3'-ddC [23-ddC]	3'
3'-dA (chain terminator) [3-dA]	3'
3'-dC (chain terminator) [3-dC]	3'
3'-dG (chain terminator) [3-dG]	3'
3'-dT (chain terminator) [3-dT]	3'
5-Pyrine Cap [5-PyrCAP]	5'	Image not available.
Acrydite [5Acrd]	5'
Cholesteryl-TEG [Chols]	3'
Cholesteryl-TEG [Chol-TEG]	5', Internal
DNP-TEG [DNP]	5', Internal
Digoxigenin (ester) [Dig]	5', 3', Internal
Phosphorothioation [*]	Internal
Puromycin [Pmycn]	3'

There are two ways to add a modification to a sequence. One, the designator can be entered directly into the sequencing prior to upload, and the system will identify the modification and include it during the sequence validation process. Secondly, each order page contains a dropdown list of available modifications for each position. Please note that designators are case sensitive.

Independent of the intended application, some modifications require further purification and/or a higher scale. Any inter-dependencies are represented on the ordering page so it is clear and transparent for customers while placing orders.

Modifications

Non-Standard Mods

How to enter modifications / designators

Requirements