NGS UDI Primers
Unique Dual Index Primer Pairs for Multiplexing on Illumina
Eurofins Genomics' NGS UDI Primer Sets are specifically designed to prevent misassignment due to index hopping.
The highlights of Eurofins Genomics' NGS UDI Primer Sets at a glance
- Utilizes 12-nucleotide long Unique Dual Indexes (UDIs)
- Minimum edit distance of four bases or more, enabling precise and accurate demultiplexing.
- Designed to prevent misassignment due to index hopping.
- Compatible with all TruSeq-based NGS library types, including those using NEB adapters.
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- Offers exceptional performance even in low-complexity pools, achieving the lowest cross-contamination rates (see application note)
- Each batch is quality controlled by our NGS facility and released only after meeting strict QC criteria.
- The general structure of the primers can be found in each plate assignment document below.
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NGS UDI Primer Sets are delivered in a full-skirted 96-well PCR plate with a pierceable foil seal. Each primer pair has a concentration of 10µM provided in bidest water in 10µl for single use. Enter the quantity you need and click "Add to Cart".
How to Order
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NGS UDI Primer Set 1
96 primer pairs for 96 reactions
Click here to download plate assignment with u7 and u5 index primer sequences.*
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NGS UDI Primer Set 2
96 primer pairs for 96 reactions
Click here to download plate assignment with u7 and u5 index primer sequences.*
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NGS UDI Primer Set 3
96 primer pairs for 96 reactions
Click here to download plate assignment with u7 and u5 index primer sequences.*
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NGS UDI Primer Set 4
96 primer pairs for 96 reactions
Click here to download plate assignment with u7 and u5 index primer sequences.*
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* For information on which u5 index orientation to use (forward or reverse complement), please refer to the Illumina page on it. Information for the use of 12 bp UDI sequences for sequencing on MiSeq systems can be found on the FAQ page.
About NGS UDI Primers
Precision Indexing for Reliable Next-Generation Sequencing
Eurofins Genomics' NGS Unique Dual Index (UDI) Primer Kits provide a powerful and reliable solution for multiplexed next-generation sequencing (NGS) applications. Designed to ensure optimal indexing accuracy, our UDI primers minimize index hopping and cross-contamination, delivering high-confidence sequencing results across a wide range of applications.
Key Features & Benefits:
- Unique Dual Indexing (UDI): Each primer set contains distinct index sequences, eliminating misassignment of reads and reducing sequencing errors.
- Validated for Illumina Platforms: Our UDI primers are optimized for seamless integration with Illumina NGS workflows, ensuring compatibility with standard library preparation protocols.
- Low Index Hopping: Proprietary design reduces the likelihood of index cross-talk, improving sequencing accuracy for high-sensitivity applications.
- High-Throughput Capabilities: Available in high-quality, high-yield formats to support large-scale sequencing projects.
- Customizable Options: Tailor-made index combinations can be provided to meet specific project requirements.
Applications
Eurofins Genomics' NGS UDI Primer Kits are ideal for a variety of NGS applications, including:
- Whole genome sequencing (WGS)
- Whole exome sequencing (WES)
- Transcriptome sequencing (RNA-Seq)
- Amplicon sequencing
- Metagenomics and microbiome profiling
- Targeted sequencing panels