AAV Sequencing

Real-time, long-read sequencing for AAV-ITR applications

Eurofins Genomics offers affordable, fast AAV sequencing using the Oxford Nanopore (ONT) sequencing platform, which is known for providing real-time, long-read sequencing. Traditional sequencing methods often struggle with the repetitive and GC-rich regions of AAV genomes, whereas ONT traverses these complexities, ensuring complete and accurate genome assemblies.

Furthermore, Eurofins Genomics can analyze multiple AAV samples in parallel. This type of scalability is critical for large-scale studies and clinical applications, enabling researchers to rapidly obtain high-quality sequencing data.

Sequence Confirmation & Validation	File Formats
Using long-read sequencing to confirm your AAV plasmid or insert, verify the full-length integrity of the recombinant AAV (rAAV) vectors, and detect any DNA contaminants during AAV library preparation. Pricing starting at $15/plasmid Same day data (measured from when the samples arrive at the lab)	.fasta file .gbk GenBank file pLannotate map in the GenBank file format. .fastq file - raw data on reads. Histogram file for visual representation of the plasmid .html pLannotate plasmid map .csv confidence file with quality statistics.

More Information

Adeno-associated virus (AAV) is popular gene transfer vector for gene therapy, amongst other applications. Gene therapy is used to treat genetic disorders by introducing corrected genes into the affected cells. However, it can be challenging to introduce a gene vector without inadvertently causing a new pathogen. AAV helps to solve this problem because it is a nonpathogenic virus with low immunogenicity.

Sample type	Size Category	Length	Concentration	Min volume	Price per sample
AAV	Regular	2.5 - 25 kb	30 ng/uL	≥10 uL	$15
	Large	25 - 125 kb	50 ng/uL	≥20 uL	$30
	XL	125 - 300 kb	50 ng/uL	≥40 uL	$60

Vector Genome Integrity

Ensuring the integrity of AAV vector genomes is crucial for therapeutic efficacy. ONT’s long-read sequencing technology can detect and characterize structural variants, deletions, and insertions within the AAV genome, providing insights into vector stability and potency. This comprehensive analysis is pivotal for optimizing vector design and manufacturing processes.

Full-length Capsid Sequence Analysis

The AAV capsid, composed of viral proteins, determines tropism and immunogenicity. Eurofins Genomics long-read sequencing can sequence full-length capsid genes without fragmentation, allowing for the precise identification of mutations, recombinations, and variants that may impact vector performance and safety.

Epigenetic Modifications

Epigenetic modifications such as methylation can influence AAV vector expression and packaging. Eurofins Genomics direct sequencing service can detect these modifications in native DNA without the need for bisulfite conversion, providing a more accurate and detailed epigenetic profile of AAV vectors.

Quality control is paramount in the production of AAV vectors for clinical use. Eurofins Genomics sequencing enables real-time monitoring of vector batches, ensuring consistency and purity. This capability reduces the risk of contamination and ensures that only high-quality vectors proceed to clinical trials.

Regulatory agencies require comprehensive characterization of gene therapy vectors. ONT’s detailed sequencing data supports regulatory submissions by providing robust evidence of vector integrity, purity, and consistency. This facilitates the approval process, accelerating the timeline from research to clinical application.

ONT technology is well suited for AAV sequencing, offering accuracy, real-time data acquisition, and scalability. Its applications in vector genome integrity analysis, full-length capsid sequencing, and epigenetic profiling make it an indispensable tool for advancing gene therapy. Eurofins Genomics has combined the advantages of the ONT platform with our veteran sequencing expertise to offer an exceptional solution for AAV sequencing. By integrating our AAV sequencing service in your research and production pipelines, researchers and clinicians can enhance the development, safety, and efficacy of next-generation gene therapies.

Testimonials

“Your sequencing reactions were perhaps the turning point in my work. Obtaining such good quality reads is rare. I have submitted many samples but this is was the best, we should explore all variable associated with the success to make it reproducible, quality, quantity of DNA to reagents used at Eurofins.”

Shyam G

Customer

“We got the sequences back and are quite happy with the quality. One of the orders had amplicons that we have been sequencing routinely for many years and quality from Eurofins seems to be better. Also, our overall experience with Eurofins website, sample submission, email notifications and sequencing results was positive and user friendly. We are looking forward to sequencing with Eurofins.”

Anna M.

Customer

“This was really impressive. Cannot believe we can get full plasmid sequencing for $15 a pop. Greatly appreciated.”

Drake R. PhD

Customer

“Whole Plasmid Sequencing is good service at a great price point that saves me time. A great addition to the sequencing toolkit that I will be using more in the future.”

Shane A

Customer

“Beautiful sequences”

Dr. Dorothy E.

Customer

“Speed of analysis is exceptional!”

Adeleye A.

Customer

“I have to say, your sequencing is outstanding. From ease of drop off to rapid turn around, to your customer support people if I do have a question and call. I refer everyone to Eurofins. Thanks for doing a great job!”

Amy H.

Customer

“Incredibly fast turnaround, and the sequences are stellar. I’m really thankful for your services, they make my job much easier.”

Joseph D.

Customer

“I am consistently surprised and pleased with how quickly you can provide sequencing data! I dropped my samples off late last evening at my local dropbox and my results were waiting in my inbox when I arrived at work this morning! My PI and I were just joking that we make a comment every time about how the results come so quickly. Maybe we just aren't used to such good customer service. Great job.”

Eva J.

Customer

“Love your service. You guys are my go to service, and I constantly recommend you to local folks who do sequencing.”

Daniel K

Customer