Whole Plasmid Sequencing

Quick Order
0

CART

Account
- Login
- Create Account

$15 / Whole Plasmid Sequencing

Same Day Results

Results delivered the same day samples are received

$15 per plasmid.

Sequencing verification for a fraction of the price

Full-Length Plasmids

Get the complete picture of your plasmid from 5kb - 30kb

Same Great Sequencing / Without the Primers

Fast, accurate, and affordable long-read sequencing

Confirm your full plasmids faster, more accurately, and more affordably than ever before. NGS generation 3 technology has made it possible to quickly sequence whole plasmids in a fraction of the time and without the hassle of having to design and synthesize primers. The typical turnaround time for whole plasmid sequencing is 1 business day measured from when the samples are received. There is no minimum requirement for the number of samples.

Order in tube Format

Order in plate format

Sample Submission Guidelines:

Sample type	Category	Size	Component #	Concentration	Min volume	Price per sample	Nanocoins
Plasmid	Regular	2.5 - 25 kb	3010-WPSPM	30 ng/uL	≥10 uL	$15	1
	Large	25 - 125 kb	3010-WPSPL	50 ng/uL	≥20 uL	$30	2
	XL	125 - 300 kb	3010-WPSPXL	50 ng/uL	≥40 uL	$60	4
Linear/Amplicon	Regular	600 bp - 25 kb	3010-WPSAM	30 ng/uL	≥10 uL	$15	1
Linear/Amplicon	Large	25 - 125 kb	3010-WPSAL	50 ng/uL	≥20 uL	$30	2
Bacterial Genome	Regular	up to 7 Mb	3010-WPSBM	50 ng/uL	≥50 uL	$90	6
Bacterial Genome	Large	7 - 12 Mb	3010-WPSBL	50 ng/uL	≥50 uL	$105	7
AAV	Regular	2.5 - 25 kb	3010-ONTAAVM	30 ng/uL	≥10 uL	$15	1
	Large	25 - 125 kb	3010-ONTAAVL	50 ng/uL	≥20 uL	$30	2
	XL	125 - 300 kb	3010-ONTAAVXL	50 ng/uL	≥40 uL	$60	4
Premium PCR Sequencing	Regular	Est. 3000 reads	3010-ONTLSM	See product info	≥10 uL	$30	2
	Large	Est. 6000 reads	3010-ONTLSL	See product info	≥20 uL	$60	4
	XL	Est. 12,000 reads	3010-ONTLSXL	See product info	≥40 uL	$120	8
Custom Sequencing	Base service	100 bp to a massive 300 kb	3010-ONTCUS	See product info	See product info	$500 up to 1 Gb	n/a
	Addl. Data		3010-ONTCUSA	See product info	See product info	$50 per addl Gb	n/a
	Barcoding		3010-ONTCUSB	See product info	See product info	$50 per sample if multiplexing	n/a

How do I ship samples?

1. Dropbox - we have a nationwide network of dropboxes. If you want a box for your lab, let us know! Email GenomicsSupport@eurofins.com to request a box.

2. Free Digital Shipping Labels - digital shipping labels are provided free for a vast majority of sequencing orders. You can select the option for digital shipping label during checkout. Take a look at our sample submission options page for more details.

3. Ship the samples using your normal carrier - If using your own carrier, we highly recommend shipping overnight/next-day delivery to ensure the samples do not degrade in transit.

How do I get results?

Results are available to download from the order history page. You will be emailed when the results are ready as well. Currently, 95% of results are delivered within 1 business day from receiving the sample.

What data files will I recieve?

Visit the Nanopore Sequencing section of the FAQ page for a list of data deliverables.

The Easiest Possible Way to / Sequence

No Primers Needed

Unlike short-read sequencing, whole plasmid sequencing does not require any primers.

No library prep needed

Unlike conventional next generation sequencing methods, no library prep is required.

No Shipping Cost

You can use our existing network of dropboxes to submit samples or print a free digital shipping label after checkout for qualifying orders.

Accuracy

Oxford Nanopore’s reports the raw read accuracy is 98.3% and the consensus accuracy for SNPs is 99.6% with 50X coverage.

Benefits / and Advantages

Benefits

- Long read recovery of several kilobases
- Great for GC rich or repetitive DNA
- Detect E. coli and genomic DNA carryover
- Faster TAT compared to traditional primer walking and plasmid verification techniques
- Lower cost than traditional NGS methods
- Verification of the entire vector sequence
- Scalable
- Available from one sample
- 5 - 4 million reads per Flow Cell (dependent on DNA quality and length)

Applications

Whole plasmid sequencing using NGS Gen 3 technology is a powerful and accurate method for characterizing and analyzing plasmids, which makes it well suited for a wide range of applications.

- Plasmid Verification
- Resequencing of whole genomes
- Assembly of genomes
- Identification of taxonomic background
- Metagenomic analysis of long reads
- Much more.

Easy to Submit

- No primers required! It could not be easier
- Requires less DNA than short-read sequencing
- Super simple order page
- Turnaround is typically 1 business day, measured from when samples arrive
- Nationwide network of dropboxes
- No dropbox near you? No problem. Eurofins Genomics is the only company that offers free digital shipping labels.