Sanger Sequencing

The Gold Standard for Sequencing

99.999% accuracy. 97.3% success rate across all applications

Eurofins Genomics is the fastest nationwide sequencing provider and outperforms other vendors on quality as measured by read length and Q20 score, according to annual benchmark comparisons. We offer the widest array of prepaid sequencing options, which empower researchers with freedom and flexibility to sequence when they want to. Let Eurofins Genomics handle your daily DNA sequencing needs so you can spend more time on research and innovation.

Overnight Sequencing		Prepaid Sequencing
Eurofins Genomics is the only sequencing provider that offers nationwide overnight results throughout the continental US. Results are delivered the same day the samples arrive, typically by 10 AM. Go to the online order page		The pioneer of prepaid sequencing with exclusive products such as SimpleSeq, Prepaid Plates, and FlexReads. These options are convenient and grant-friendly. Buy now, use later.

Regulatory Sequencing		Direct Colony Sequencing
GLP and GMP sequencing is the gold standard approach for attaining high-confidence sequence data, especially for preclinical and regulated studies. By utilizing a stringent process control and QA framework, we guarantee the reliability and reproducibility of the data generated, ensuring accurate interpretation and decision-making. GLP Sequencing Information l Order GMP Sequencing Information l Order		Submit bacterial samples for sequencing using rolling circle amplification (RCA) to obtain high-quality genomic information directly from bacterial or fungal colonies. Eurofins Genomics accepts Bacterial Colonies, Glycerol Stock / Bacterial Culture, and Bacterial Pellet for direct colony sequencing. Order are placed on the normal sequencing submission pages: submit in tubes, submit in plates.

Specifications

	Submit Online Order Submit Tubes Submit Plates

	Prepare Samples Sample Submission Guidelines Order Supplies Print Shipping Label from Order Confirmation Page

	Drop-off / Ship Samples Customers can drop samples in a local dropbox or ship the samples using our free digital shipping labels. Find a UPS dropbox near you.

	Access Results the Following Day Results are downloaded from the My Orders page. Click the order to expand and download the results, when available. Typically results appear by noon, even on Saturdays.

Stop waiting. Start sequencing

Overnight DNA Sequencing - Anywhere in the US!

Eurofins Genomics offers nationwide overnight results for all sequencing services in the US. When you place an online order and send us your samples using the free overnight shipping labels, you'll receive your results the next morning, even on Saturday! (Overnight sequencing is available for orders up to 192 standard sequencing reactions.)

Anywhere in the US, including Alaska and Hawaii
Premium service, same low price
Results the next morning, including Saturday
Free Shipping on >90% of orders, through digital shipping label

US_EG-JSC

Eurofins Nationwide

Nationwide Competitors

It is important to note that some competitors do offer nationwide overnight service if the customer pays for overnight shipping and certain restrictions are met. In our opinion, this is not true overnight service due to the cost and time constraints around such as service. The comparison above is an "apples to apples" comparision when taking into consideration the true cost and time required.

Compare Cost

Eurofins

Other Providers

Shipping Cost

Free for >90% orders

Prepaid, digital shipping labels provided

$20–34

Customer needs to pay for shipping

Turnaround Time

Overnight on tube orders*

2-3 days on large orders

2-5 days depending on logistical options and size of order

*Some restrictions apply.

	Barcoded Tubes 1.5 – 2.0 mL Centrifuge tubes, with or without barcodes. Fastest turnaround time, along with SimpleSeq tubes. Required for overnight sequencing. Eurofins Genomics provides bar-code labels for use with templates and primers that are submitted for DNA sequencing services. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins Genomics for DNA sequencing has a unique identifier that is tracked throughout our automated system. Submitting online: Enter the barcode on the tube submission page. All tube types use the same sequencing tube submission page.

	Non-Barcoded Tubes 1.5 – 2.0 mL Centrifuge tubes, with or without barcodes. Non-barcoded tubes are processed after barcoded tubes, so the turnaround time might be impacted slightly. Additional fees may apply for not using barcodes as it requires more labor in the lab to track samples. This depends on your institutional agreement with Eurofins Genomics. Submitting online: All tube types use the same sequencing tube submission page.

	8 Strip PCR Tubes Variety of sizes. If submitting less than 8, please cut the strip and save the remaining tubes Submitting online (new process): 8-strip barcodes are automatically generated by our system and displayed on the online order page. Please write these barcodes on the side of your tubes using a sharpie marker. If using the excel upload form, please leave the barcode field empty. The barcodes are a combination of your initals and a number sequence, such as DR01...DR02...et cetera. All tube types use the same sequencing tube submission page.

	SimpleSeq Tubes Pre-labeled Tube size is 0.75 mL All tubes provided with kit Fastest turnaround time, along with normal barcoded tubes. Submitting online: All you need to do is type in the barcode when submitting online. All tube types use the same sequencing tube submission page, except for SimpleSeq Premixed which requires no online entry.

Go to the Plate Sequencing page for details and specifications on all plate options.

Eurofins Genomics offers discounted pricing for plate orders with at least >24 samples, and the most flexible sample submission and customization of plate orders. Plate sequencing includes a wide array of submission options including Standard Plates, Prepaid Plates, ShortSeq Plates, and Ready2Load Plates.

Submit Samples in Plates

Plate Types

Plate Format

>48 samples

Submit Standard and Prepaid Plates

Order Prepaid Plates

Submit ShortSeq

Submit Ready2Load

Sequencing Standard 96-well Plate

Standard 96-well plates, fully skirted
Plasmid and PCR
Flexible options
Price and turnaround time based on volume

Prepaid Plate Kit

96 sequencing reactions provided with the plate kit (prepaid)
Free when submitting the plate later
Plasmid and PCR
A colored, barcoded PCR plate is provided
Twelve 8-cap strips for sealing provided

ShortSeq Plate

Plate must be fully skirted
Lower cost than normal plates
Results arrive typically by noon the next morning
Shorter read lengths due to faster run, typically <500 bp.
Premixed only. Please use tightly capped plates to prevent contamination.

Ready2Load Plate

Plate must be fully skirted
Post BigDye, must be ready to analyze on arrival
Lower cost than normal plates
Please use tightly capped plates to ensure no contamination.

Barcodes are used to track samples and primers in the lab. Customers who use barcodes receive a faster turnaround time. Customers enter these barcodes on the online submission page(s) to link the order to the tubes. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins Genomics for DNA sequencing has a unique identifier that is tracked throughout our automated system.

Eurofins Genomics provides barcode labels for free. These barcodes can be applied to sample tubes and/or primer tubes. Free barcodes can be ordered on the supplies page. In addition, all SimpleSeq kits come with prelabeled tubes and extra barcodes for primer tubes.

Submitting Barcodes on the Online Order Page

For free barcodes and SimpleSeq barcodes, enter the barcode on the side of the tube into the barcode field on the submission page. Standard and SimpleSeq barcodes can only contain digits. Please remove any hyphens, dashes, spaces, or periods before using a barcode.
8-strip barcodes are automatically generated on the order page using your initials, i.e. John Doe would generate barcode JD1, JD2, etc. You do not need to order physical barcode labels if choosing 8-strip PCR tubes.

Need help finding your barcode? CLICK HERE to open your Sequencing Dashboard which easily displays all barcodes in your account.

Additional Information

Always adhere to sample submission guidelines to ensure that you receive the best sequence data with the shortest turnaround time.
Read lengths are typically >1000 although obviously results depend on sample and primer quality
Specific primers can be requested or enclosed
Over 80 free standard primers available
Template and primer may be sent premixed
Accuracy of data is greater than 99% (Q20 or better)
Prepayment options are available not only through SimpleSeq, but also with EVOcards

Requirements to Qualify for the Overnight Sequencing Service:

Samples must be sent using Eurofins Genomics digital shipping label, which are provided during checkout, and dropped off before the cut off time for next day air (UPS). Using any shipping method except for UPS overnight will not qualify for overnight sequencing.
Samples assembled in tubes or plates supplied by Eurofins Genomics (e.g., SimpleSeq Kits and Prepaid Plates) or barcode-labeled custom centrifuge tubes (≥1.5 mL tubes or semi-skirted plates).
Maximum of 192 sequencing reactions.
Only standard read conditions (sequencing projects that require additional services, such as PCR cleanup, Power Read, RCA, BAC reads, or primer walking are not included in the overnight sequencing service).
Samples must be prepared according to the guidelines provided in the Sample Submission Guidelines including the use of barcode labels.

Non-adherence to any of the above sample preparation and submission processes, including the absence of barcode labels, can result in delays. Once the samples are assembled in barcode labeled tubes and plates, please follow the four simple steps to get the sequencing results by noon of the following day

How to download results?

Go to your Order History. Next, click the order to expand and reveal the download link. Click the link to download a zip file of the results. To visit the Order History page, click one of the many links located throughout the website, including in the header beside your account name.

When looking at the order history page, each type of order is color coded by its product line, so it is easy to find the order you want. For example, sequencing orders are marked by a small blue icon, oligo orders are marked by an orange icon, and so forth. Please note that sequencing results are only saved for a limited amount of time. If you are trying to download very old results, it may not be available online but may be archived and available by manual retrieval. Contact us for more information.

File types available for Sanger Results:

phd_Resource
*ab1
*pdf
*scf
scq_Resource

Select the file types you wish to receive with sequencing results .zip file

Selecting Preferred File Types

Users can select which file types to receive during the "Preferences" step of checkout. Simply check the box beside the desired file types.

In the genomics era, where the unlimited possibilities of discovering new genetic frontiers are limited only by your budget and time, we have made enhancements to our oligo portfolio to allow you to reach a little further faster and within your budget and time. Don't sacrifice speed or quality in your pursuit of groundbreaking science.

Testimonials

“It was good and convenient service. The sequencing results were great. I could just drop off the samples in the same building where I work. The results were very fast also, only one day." ★★★★★”

D. Prakoso

Customer

“Your sequencing reactions were perhaps the turning point in my work. Obtaining such good quality reads is rare. I have submitted many samples but this is was the best, we should explore all variable associated with the success to make it reproducible, quality, quantity of DNA to reagents used at Eurofins.”

Shyam G

Customer

“I've already strongly recommended Eurofins Genomics to a colleague in my department and they are now using the sequencing service. I was amazed at the turnaround time for prepaid plates and the quality far exceeds anything I've received from our in-house core facility. The quality and speed of the service combined with the price for the pre-paid plates has completely changed my lab's sequencing routine for the better.”

Herman M.

Academic

“Service has always been great!”

David L. Remington

Academic

“Fast accurate service. Very reliable...excellent customer service. Every time I call you are helpful. I bet everything else you do is as good as your sequencing service.”

Andrew K.

Researcher

“Always fast, always helpful, always the most rapid service provider.”

Ricardo B.

Research Scientist

“I am consistently surprised and pleased with how quickly you can provide sequencing data! I dropped my samples off late last evening at my local dropbox and my results were waiting in my inbox when I arrived at work this morning! My PI and I were just joking that we make a comment every time about how the results come so quickly. Maybe we just aren't used to such good customer service. Great job.”

Eva J.

Customer

“Sequencing is getting done faster than before, with same high quality. The sequencing quality has been constantly superior and I have been able to download the data at 8-9am the second day. Also customer service reps are extremely helpful and professional. Thanks!”

Wenzhen D.

PROFESSOR

“We got the sequences back and are quite happy with the quality. One of the orders had amplicons that we have been sequencing routinely for many years and quality from Eurofins seems to be better. Also, our overall experience with Eurofins website, sample submission, email notifications and sequencing results was positive and user friendly. We are looking forward to sequencing with Eurofins.”

Anna M.

Research Scientist

“Brilliant sequencing services.”

I. Osekre

BA, MBA