Sanger Sequencing

Stop waiting. Start sequencing

Overnight DNA Sequencing - Anywhere in the US!

Eurofins Genomics offers nationwide overnight results for all sequencing services in the US. When you place an online order and send us your samples using the free overnight shipping labels, you'll receive your results the next morning, even on Saturday! (Overnight sequencing is available for orders up to 192 standard sequencing reactions.)

• Anywhere in the US, including Alaska and Hawaii
• Premium service, same low price
• Results the next morning, including Saturday
• Free Shipping on >90% of orders, through digital shipping label

Eurofins Nationwide	Nationwide Competitors

It is important to note that some competitors do offer nationwide overnight service if the customer pays for overnight shipping and certain restrictions are met. In our opinion, this is not true overnight service due to the cost and time constraints around such as service. The comparison above is an "apples to apples" comparision when taking into consideration the true cost and time required.

Compare Cost

*Some restrictions apply.

How to download results?

Go to your Order History. Next, click the order to expand and reveal the download link. Click the link to download a zip file of the results. To visit the Order History page, click one of the many links located throughout the website, including in the header beside your account name.

When looking at the order history page, each type of order is color coded by its product line, so it is easy to find the order you want. For example, sequencing orders are marked by a small blue icon, oligo orders are marked by an orange icon, and so forth. Please note that sequencing results are only saved for a limited amount of time. If you are trying to download very old results, it may not be available online but may be archived and available by manual retrieval. Contact us for more information.

File types available for Sanger Results:

• phd_Resource
• *ab1
• *pdf
• *scf
• scq_Resource

Select the file types you wish to receive with sequencing results .zip file

Selecting Preferred File Types

Users can select which file types to receive during the "Preferences" step of checkout. Simply check the box beside the desired file types.

Tube Types and Specifications

	Barcoded Tubes 1.5 – 2.0 mL Centrifuge tubes, with or without barcodes. Fastest turnaround time, along with SimpleSeq tubes. Required for overnight sequencing. Eurofins Genomics provides bar-code labels for use with templates and primers that are submitted for DNA sequencing services. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins Genomics for DNA sequencing has a unique identifier that is tracked throughout our automated system. Submitting online: Enter the barcode on the tube submission page. All tube types use the same sequencing tube submission page.
	Non-Barcoded Tubes 1.5 – 2.0 mL Centrifuge tubes, with or without barcodes. Non-barcoded tubes are processed after barcoded tubes, so the turnaround time might be impacted slightly. Additional fees may apply for not using barcodes as it requires more labor in the lab to track samples. This depends on your institutional agreement with Eurofins Genomics. Submitting online: All tube types use the same sequencing tube submission page.
	8 Strip PCR Tubes Variety of sizes.  If submitting less than 8, please cut the strip and save the remaining tubes Submitting online (new process): 8-strip barcodes are automatically generated by our system and displayed on the online order page. Please write these barcodes on the side of your tubes using a sharpie marker. If using the excel upload form, please leave the barcode field empty. The barcodes are a combination of your initals and a number sequence, such as DR01...DR02...et cetera. All tube types use the same sequencing tube submission page.
	SimpleSeq Tubes Pre-labeled Tube size is 0.75 mL All tubes provided with kit Fastest turnaround time, along with normal barcoded tubes. Submit SimpleSeq and SimpleSeq Premixed tubes on the online tube submission page. Enter the tube barcode in the barcode field.

Submit Samples in Tubes

Plate Types and Specifications

Go to the Plate Sequencing page for details and specifications on all plate options.

Eurofins Genomics offers discounted pricing for plate orders with at least >24 samples, and the most flexible sample submission and customization of plate orders. Plate sequencing includes a wide array of submission options including Standard Plates, Prepaid Plates, ShortSeq Plates, and Ready2Load Plates.

Plate Types

Plate Format >48 samples Submit Standard and Prepaid Plates Order Prepaid Plates Submit ShortSeq Submit Ready2Load		Sequencing Standard 96-well Plate Standard 96-well plates, fully skirted Plasmid and PCR Flexible options Price and turnaround time based on volume
		Prepaid Plate Kit 96 sequencing reactions provided with the plate kit (prepaid) Free when submitting the plate later Plasmid and PCR A colored, barcoded PCR plate is provided Twelve 8-cap strips for sealing provided
		ShortSeq Plate Plate must be fully skirted Lower cost than normal plates Results arrive typically by noon the next morning Shorter read lengths due to faster run, typically <500 bp. Premixed only. Please use tightly capped plates to prevent contamination.
		Ready2Load Plate Plate must be fully skirted Post BigDye, must be ready to analyze on arrival Lower cost than normal plates Please use tightly capped plates to ensure no contamination.

Submit Samples in Plates

Barcodes are used to track samples and primers in the lab. Customers who use barcodes receive a faster turnaround time. Customers enter these barcodes on the online submission page(s) to link the order to the tubes. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins Genomics for DNA sequencing has a unique identifier that is tracked throughout our automated system.

Eurofins Genomics provides barcode labels for free. These barcodes can be applied to sample tubes and/or primer tubes. Free barcodes can be ordered on the supplies page.  In addition, all SimpleSeq kits come with prelabeled tubes and extra barcodes for primer tubes.

Submitting Barcodes on the Online Order Page

• For free barcodes and SimpleSeq barcodes, enter the barcode on the side of the tube into the barcode field on the submission page. Standard and SimpleSeq barcodes can only contain digits. Please remove any hyphens, dashes, spaces, or periods before using a barcode.
• 8-strip barcodes are automatically generated on the order page using your initials, i.e. John Doe would generate barcode JD1, JD2, etc. You do not need to order physical barcode labels if choosing 8-strip PCR tubes.

Need help finding your barcode?  CLICK HERE to open your Sequencing Dashboard which easily displays all barcodes in your account.

 Additional Information

• Always adhere to sample submission guidelines to ensure that you receive the best sequence data with the shortest turnaround time.
• Read lengths are typically >1000 although obviously results depend on sample and primer quality
• Specific primers can be requested or enclosed
• Over 80 free standard primers available
• Template and primer may be sent premixed
• Accuracy of data is greater than 99% (Q20 or better)
• Prepayment options are available not only through SimpleSeq, but also with EVOcards

Requirements to Qualify for the Overnight Sequencing Service:

• Samples must be sent using Eurofins Genomics digital shipping label, which are provided during checkout, and dropped off before the cut off time for next day air (UPS). Using any shipping method except for UPS overnight will not qualify for overnight sequencing.
• Samples assembled in tubes or plates supplied by Eurofins Genomics (e.g., SimpleSeq Kits and Prepaid Plates) or barcode-labeled custom centrifuge tubes (≥1.5 mL tubes or semi-skirted plates).
• Maximum of 192 sequencing reactions.
• Only standard read conditions (sequencing projects that require additional services, such as PCR cleanup, Power Read, RCA, BAC reads, or primer walking are not included in the overnight sequencing service).
• Samples must be prepared according to the guidelines provided in the Sample Submission Guidelines including the use of barcode labels.

Non-adherence to any of the above sample preparation and submission processes, including the absence of barcode labels, can result in delays. Once the samples are assembled in barcode labeled tubes and plates, please follow the four simple steps to get the sequencing results by noon of the following day