NGS UDI (Unique Dual Index) Primer Sets for Multiplexing on Illumina
Since its first introduction in the mid-2000s, Next-Generation Sequencing (NGS) has become significantly cheaper and more widely utilized. Uses for NGS range broadly, from whole genome sequencing to targeted DNA/RNA sequencing. The COVID-19 pandemic brought the technology into the mainstream as NGS was utilized for viral genome analysis for variant detection. The higher usage of NGS has brought the need for solutions to increase throughput. Unique Dual Indexes (UDIs) allow for the multiplex sequencing on NGS platforms and subsequent demultiplexing during data analysis. Multiplex sequencing has requires highly pure, low cross-contaminated indexes as a single swap may lead to cross-talk.
Eurofins Genomics uniquely provides custom solutions ensuring that the sequence data generated is a highly accurate reproduction of the original sequence of the DNA of your sample. These proprietary technologies start with our high-fidelity synthesizers that can produce oligos up to 200bases in length, at both small to intermediate scales. The Hi-Fi synthesis coupled with workflows that spatially and temporally separate oligos significantly minimizes the chances for well-to-well contamination.
Eurofins Genomics has seen a huge increase in demand for custom UDI sets. Furthermore, customers have been extremely pleased with the performance of our custom UDIs compared to alternative vendors, which is driving organic growth. So we have reallocated resources from synthesizing stock UDIs onto building custom indexing primers to fill the demand. Stock UDI sets should return to the website towards the end of 2022.”
Eurofins Genomics' NGS UDI Primer Sets to eliminate misassignment by index hopping.
- Ideal for all NGS libraries types including libraries produced with NEB adapters
- Unique pairs of u5 and u7 high performance index primers
- UDIs for standard indexing (8 nucleotides)
- Very high performance also for low-plexity pools
- Minimum edit distance of three bases or higher
Each primer pair has a concentration of 10µM provided liquid in 10µl for single use.
5` linker sequence for target-specific primers:
u5 5'-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3'
u7 5'-GACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3'
Note: U5 and U7 contain the complete adapter, whereas I5 and I7 are the short indices of Illumina.
NGS UDI Primer Sets are delivered in a single-use fully-skirted 96-well PCR plate with a pierceable foil seal. Just select your required NGS UDI Primer Set with required quantity and press "Add to Cart". The general structure of the primers can be found in each plate assignment document below.
| Kit |
Description |
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NGS UDI Primer Set 96-1
Out of Stock-New Version Coming Soon!
96 primer pairs for 96 reactions
Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.
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NGS UDI Primer Set 96-2
Out of Stock-New Version Coming Soon!
96 primer pairs for 96 reactions
Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads
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NGS UDI Primer Set 48
Out of Stock-New Version Coming Soon!
48 primer pairs for 48 reactions
Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.
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NGS UDI Primer Set 24-1
Out of Stock-New Version Coming Soon!
24 primer pairs for 24 reactions
Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.
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NGS UDI Primer Set 24-2
Out of Stock-New Version Coming Soon!
24 primer pairs for 24 reactions
Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.
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