Concentrate your studies on the exome part of the genome

Benefit from a cheaper, faster and still efficient strategy in comparison to whole genome sequencing.

Most disease causing variants lie within the exonic regions, splice sites, or promotor regions. These regions comprise about 2% of the human genome. Sequencing the exome provides a cheaper and faster analysis of these regions compared to whole genome sequencing. Exome sequencing is an efficient strategy for reading the parts of the genome that are believed to be the most important for diagnosing diseases.

On routine Eurofins Genomics offers whole exome sequencing using the newest Agilent human all exon V6 exome kits in combination with Illumina HiSeq 2500 sequencing.

The Agilent V6 kits feature:

• Highly uniform coverage of the exome
• Up-to-date content
• SureSelect Human All Exon V6 (60 Mbp; coding regions only)
• SureSelect Human All Exon V6 + UTRs

Other solutions that are offered for your research are:

• NimbleGen EZ Exome and EZMed Exome
• Illumina Nextera Rapid Exome

You can order our capture and sequencing service in combination with the proprietary Eurofins exome analysis pipeline. Receive mapping files, variant annotation and interactive visualization of mapping results in your online genome browser.

Get the greatest flexibility and select the best suitable approach for your research. We would be happy to consult you about the most convenient strategy for your exome project. Just contact us!

Hybridisation-based Exome Capturing with NimbleGen, Illumina and Agilent

For human exome resequencing, predesigned and hybridization based exomes are available from Roche NimbleGen, Illumina and Agilent. Eurofins Genomics offers the enrichment with the newest available exome versions, sequencing, and data analysis as a convenient and state of the art one-stop solution.

NimbleGen EZ Exome Designs

• Flexible probe sizes: 50-105-mers
• 1.2 million probes provide high coverage uniformity
• SeqCap EZ Human Exome Library v3.0 (64 Mbp)

Agilent SureSelect Exome designs

• 120-mer probes
• SureSelect Human All Exon V5 (50.4 Mbp; coding regions only)
• SureSelect V5+UTRs (74.6 Mbp; coding regions plus UTRs)

Illumina Nextera Exome designs

• 95-mer probes
• Illumina Nextera Rapid Exome (62 Mbp)
• Capturing possible with DNA amounts starting from 50 ng

Choose either the high-throughput or the rapid run mode on the Illumina HiSeq 2500 for sequencing of your exomes.

You can order our capture and sequencing service in combination with the proprietary Eurofins exome analysis pipeline (including BWA mapping and GATK variant analysis). Receive mapping files, variant annotation and interactive visualization of mapping results in your online genome browser.

Are you interested in an individual quote or need more information? Just contact us!

PCR-based Exome Capturing with Ion AmpliSeq

Eurofins Genomics is pleased to be able to offer you whole exome enrichment with Ion AmpliSeq. The Ion Proton system combined with the Ion AmpliSeq technology offers fast, simple and high-quality exome sequencing.

• 294’000 primers pairs that target a total of ~ 58 Mbp
• >97% of CCDS with 5 bp padding around exons
• Sequencing on the Ion Torrent Ion PI Chip v2
• Service is offered for samples with as low as 250 ng input DNA

Need more information for your specific project? Just contact us!