NGSelect Ready-2-Load
Convenient Illumina sequencing of ready-to-load libraries (RTL)
NGSelect RTL is designed to achieve maximum speed, cost-efficiency and easy-of-use for customers with ready-to-load libraries (RTLs). Scientists can submit their ready libraries and receive their results within a few business days. Through multiplexing and pooling of samples the efficiency and cost-effectiveness of an Illumina sequencing run is significantly increased.
Applications
Ready-to-sequence libraries provide distinct advantages for researchers who are performing Illumina next-generation sequencing frequently:
- More affordability - cost-effectiveness for power users of NGS, especially through multiplexing options
- Faster results - significant improvement in data delivery times
- Decreased variability – from semi-automated processes and optimized use of Illumina flow cells
Highlights NGSelect RTL
- Results within as little as five business days
- Wide range of sequencing options: choose from different reads modes, read numbers, and delivery time options
- Extensive expertise in sample handling: semi-automated processes and optimised use of Illumina flow cells
- Sequencing workflows with ISO17025 accreditation
- Sorting by Illumina Index read at no additional costs
- Accurate determination of library concentration and appropriate sequencing dilution
NGSelect RTL offers extraordinary fast and reliable sequencing of ready-to-load libraries. Benefit from extensive expertise in sample handling, proprietary protocols, as well as professional Bio-IT analysis.
Product details
Accepted starting material
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Library Type
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Starting Material
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| Standard ready-to-load library (compatible with Illumina sequencing and index primer) |
Quantity: 300 ng per sample
Concentration: > 10 ng/µl (maximum volume 30 µl)
Dissolved in: RNase-, DNase- and protease-free Tris-HCl buffer (ph 7.5 - 8.0)
Total size (insert size and sequencing adapters):
- Ideal: 250 bp to 450 bp for 240M and 280M read packages
- Ideal: 250 bp to 600 bp for 12M and 22M read packages
- Absolute minimum: 150 bp
- Absolute maximum: 800 bp
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Specifications
Library types
- Standard ready-to-load library (compatible with Illumina sequencing and index primer)
Sequencing platforms
Data packages
- Illumina data packages ranging from 12 million to 280 million reads
Sequencing mode
- 50 bp single-read
- 100 bp single-read
- 150 bp paired-end
- 250 bp paired-end
- 300 bp paired-end
Bioinformatics analysis
- Data sorting by Illumina Index read (at no additional costs)
- Merging of overlapping paired-end reads (where applicable)
- Data sorting according to customer supplied tag
Deliverables
- FastQ Files (sequences and quality scores)
Delivery time
- Starting from five business days, but varies according to individual requests