siRNA siMAX Oligos
CLEAN RESULTS ● EFFECTIVE SILENCING ● CLEAR CHOICE
Empower your RNAi gene silencing experiments! Under another name, Eurofins Genomics pioneered synthesis improvement for siRNA synthesis—we have the experience to empower your research.
Effective silencing is most readily achieved through delivery of a guide strand with high fidelity to the siRNA and microRNA seed regions. To accelerate your research we developed extensive quality control procedures to confirm identity of each component of the siRNA duplex. Allow us to help you achieve the best in RNA silencing—a RNAi sense strand (passenger strand) and the RNAi antisense strand (guide strand) with the highest quality.
You shouldn’t have to choose between the best reagents for your gene silencing experiments and the health of your lab budget. By providing the highest quality and excellent value, siMAX siRNA empowers every RNAi gene silencing experiment it's used in.
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siMAX siRNA Specifications
Each siMAX siRNA duplex is provided as a quality-assured siRNA duplex with a purity level of 85% or greater. After passing all quality control tests, 20 nmoles of both the sense and antisense RNA oligos are placed into a tube, annealed, and then shipped as dried material to your lab.
Accelerate your research with our “fully annealed” siMAX siRNA duplexes. Our protocol for resuspension of the dried duplex is designed to make sure that the oligos are reannealed just prior to use. You may use our siRNA Dilution Buffer (included free with each shipment along with resuspension instructions) or your own preferred buffer to prepare the siRNA duplex for further use.
Enjoy the benefit of CLEAN RESULTS in your RNAi gene silencing experiments. ALL of our siRNA duplexes are delivered ready-to-use in your gene silencing experiments at 85% purity or higher.
- HPLC purified
- 20 or 40 μmol qty
- Double stranded sequence from 17–27 bases
- Annealed & ready-to-use custom siRNA!
Pricing
| Quantity delivered |
Price |
| 20 nmol |
$500.00 |
| 40 nmol |
$1,000.00 |
Modification pricing can be found on the oligo price list page.
Details
Avoid RNA degradation by using special precautions and by wearing protective lab clothing and gloves. All solutions should be qualified as RNAase-free and reserved only for use in RNA experiments. All RNA work spaces must be free of RNAase contamination. If you have any questions, please contact our Genomics Support team at 256-704-8200 or toll-free at 800-688-2248.
- Add 1 mL of siRNA Dilution Buffer to the lyophilized siRNA to create a 20 µM stock solution. (siRNA Dilution Buffer, pH 8.0 is a TRIS-EDTA buffered solution for use and/or storage of siRNA.)
- Heat the tube to 194 °F for 60–90 seconds.
- Incubate at 98 °F for at least one hour.
- The siRNA duplex 20 µM Stock solution is now ready for use.
siRNA silencing experiments have yielded a large number of siRNA complexes that are validated through refereed publications. Our siMAX siRNA library includes gene silencing siRNAs from siRecords, a research study which provides a library of RNAi siRNA catalogued by reported efficacy.
In addition to the validated siRNA library, a large number of pre-designed siRNA oligos for over 17,000 reviewed genes are offered. Control duplexes and siRNA sequences are based on the NCBI Refseq database (146.4) for mouse, rat and human.
5' Mods for siMAX siRNA Duplexes
- Fluors
-
| Fluorophore |
Code |
5' |
Int |
3' |
Max Abs |
Max Em |
Extinction coefficient |
QY†† |
| Cyanine3 dye |
[Cy3] |
✓ |
|
|
547 |
563 |
136,000 |
0.15 |
| Cyanine5 dye |
[Cy5] |
✓ |
|
|
646 |
662 |
250,000 |
0.3 |
| 6-FAM |
[FAM] |
✓ |
|
|
495 |
521 |
75,000 |
0.9 |
| HEX |
[HEX] |
✓ |
|
|
537 |
556 |
96,000 |
0.7 |
| TET |
[TET] |
✓ |
|
|
519 |
539 |
86,000 |
0.9 |
| †† QY (quantum yield) is a calculated ratio between the number of photons emitted and the number of photons absorbed. The most desireable QY for DLP purposes approaches 1. |
- Attachment Mods
-
| Attachment Mods |
Code |
5' |
Int |
3' |
Structure |
| Amino Modifier C6 |
[AmC6] |
✓ |
|
|
link |
| Phosphate |
[PHO] |
✓ |
|
✓ |
link |
- Non-flour labels
-
| Non-flour labels |
Code |
5' |
Int |
3' |
Structure |
| Biotin |
[BIO] |
✓ |
|
|
link |
| Biotin-TEG (5' or 3') |
[BIOTEG] |
✓ |
|
|
link |
Related Links
Scale and Yield Guaranteed yields for unmodified oligos at different scales NOTE: Plate-format oligos are available in the 10 nmol, 25 nmol, 50 nmol, and 0.2 μmol scales. These oligos are delivered in 96-well plates and concentration normalized at 50 µM in 200 µL or lyophilized. If you would like to place a larger order, please get in touch with our friendly Customer Service team.
Testimonials
“Best Oligo ordering system I've ever used, and I've been ordering oligos since 1992!”
“I have been using Eurofins since the past 20 years as a Lab Manager of an academic research lab in Toronto. I have found Eurofins Oligo service to be excellent in all respects. Great job Team Eurofins :)”
“Great oligonucleotides, especially at longer lengths. Eurofins is able to process primers longer than 60 mers without requiring much longer involvement in terms of price and time.”
“I've been using Operon (now Eurofins) since 1999. I've tried other vendors, but neither the quality nor the service ever matched that of Eurofins.”
“You make the best primers. I love that you're able to get them to me in 24 hours with next-day shipping on oligos <= 40 bp. Free shipping, too. I've used your service for 4 of our lab's upcoming papers, and the quality is outstanding.”
“Always a pleasure doing business with you! Excellent service and turnaround times! I have worked with Eurofins for 10 years, ever since I was a graduate student. My current lab used to order oligos and sequencing from other suppliers but I convinced them to switch to Eurofins. I advocate for you regularly to all of my lab colleagues.”
“We have been struggling with oligo quality from other providers. The ones we received from you are far and away the highest quality we have observed and at much higher yields and uniformity than we have seen elsewhere. We are incredibly impressed with the quality and have already started recommending Eurofins to collaborators and other colleagues.”
“Excellent quality oligo synthesis services”
“Easy to navigate and order oligos. I order multiple oligos at once, each with similar specifications—purification, scale, and more—but with different sequences. The ability to set the default values after entering the first oligo made the process much faster and easier. I especially liked that feature.”
“Easy to navigate and order oligos. I order multiple oligos at once, each with similar specifications—purification, scale, and more—but with different sequences. The ability to set the default values after entering the first oligo made the process much faster and easier. I especially liked that feature.”
RNA
Custom RNA and Chimeric (DNA/RNA) oligos