Summary

File type Name WPS Amplicon Bacterial Genome AAV Premium
PCR
Custom Sequencing
.html Analysis Report Yes Yes Yes Yes no no
.fasta, .gbk Consensus sequence Yes Yes Yes Yes no no
.html Plasmid map, pLannotate interactive map Yes Yes Yes Yes no no
.png Real length histogram Yes Yes Yes Yes no no
.png Coverage plot Yes Yes Yes Yes no no
.csv Per-base data / Confidence file.csv Yes Yes Yes Yes no no
.fastq Raw read sequenced Yes Yes Yes Yes Yes Yes
.html Nanoplot Summary Report Yes Yes Yes Yes no no


File Types / Data Deliverables

Convenient report that contains the most important data about your sample including the map, histogram, coverage plot, deliverables and summary information.

The NanoPlot report is a unique file that contains a summary of the results, quality scores, and histograms. 

Refined consensus sequence of the plasmid.

File type: .fasta, .gbk

 

Comprehensive, annotated plasmid map. For linear plasmids, the map may appears circular even though it is not. This is relic of the ONT assembly program.

File type: .html (pLannotate)

Visualization of raw sequencing read lengths. This distribution plot helps with sample characterization and troubleshooting. We provide several different histogram files to provide the full picture.

File type: .png

Coverage profile, showing sequencing depth across every base of the consensus sequence.

File type: .png / .gbk

Summary of each base. Includes the position, base, matches, mismatches, insertions, deletions, and more.

File type: .csv, .txt

Individual raw read sequences. For most sample types, the fastq files will only contain the sequences that align with the consensus. 

 

File type: .fastq

 

 

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Frequently Asked Questions                             Sample Submission Guidelines

 

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