Linear Amplicon Sequencing
Size & Pricing
Go to Sample Submission Guide and General FAQ >
| Sample type |
Size Category |
Length |
Concentration |
Min volume |
Price per sample |
| Linear/Amplicon |
Standard |
600 bp - 25 kb |
30 ng/uL |
≥10 uL |
$15 |
| Big |
25 - 125 kb |
50 ng/uL |
≥20 uL |
$30 |
*Eurofins Genomics uses the fragmentation (sequencing adapter attachment) method, not the ligation method, for library preparation.
About Linear / Amplicon Sequencing
Oxford Nanopore technology represents a paradigm shift in the field of genomic sequencing, and its application to amplicon sequencing has yielded profound benefits. At the heart of this innovation is the principle of single-molecule sequencing, which distinguishes it from traditional methods and imparts several advantages.
Amplicon sequencing, a technique focused on the targeted amplification of specific DNA regions, benefits significantly from the long-read capabilities of Oxford Nanopore sequencing. Unlike short-read technologies, such as those based on Illumina platforms, Oxford Nanopore sequencing generates reads of exceptional length. This feature is particularly advantageous for amplicon sequencing, as it enables the sequencing of entire target regions in a single, contiguous read. This characteristic is instrumental in resolving complex genomic regions, repetitive sequences, and structural variations, which are often challenging for short-read technologies to accurately capture.
Moreover, the real-time data acquisition capability of Oxford Nanopore sequencing provides researchers with the unique ability to monitor the sequencing process and turnaround results quickly. The versatility of Oxford Nanopore technology further extends to its adaptability for various sample types, including complex environmental samples or low-input DNA samples. This flexibility is crucial for amplicon sequencing applications where the quality and quantity of starting material may vary. The technology's ability to generate high-quality data from limited input DNA opens avenues for exploring diverse biological samples and extends the applicability of amplicon sequencing to a broader range of research domains.
Our linear/amplicon sequencing service is designed for the meticulous sequencing of clonal linear DNA ranging from 600 bp to 125 kb in length. Employing the cutting-edge long-read sequencing technology by Oxford Nanopore Technologies (ONT), this service encompasses the following key components:
- Creation of an amplification-free long-read sequencing library utilizing the latest v14 library prep chemistry. This process ensures minimal fragmentation of the linear input DNA in a sequence-independent manner.
- Primer-free sequencing of the library using the highly accurate R10.4.1 flow cells. Raw data, boasting an accuracy exceeding 99%, is meticulously delivered in .fastq format.
- Reconstruction of raw reads and their alignment against each other to generate a linear consensus sequence with a high degree of accuracy.
In a vast majority of cases, the turnaround time for linear/amplicon sequencing is the same as whole plasmid sequencing, which typically delivers results the same day the samples arrive at the lab. Our commitment is to provide you with precise, efficient, and timely insights into your genetic data.
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Testimonials
“Your sequencing reactions were perhaps the turning point in my work. Obtaining such good quality reads is rare. I have submitted many samples but this is was the best, we should explore all variable associated with the success to make it reproducible, quality, quantity of DNA to reagents used at Eurofins.”
“We got the sequences back and are quite happy with the quality. One of the orders had amplicons that we have been sequencing routinely for many years and quality from Eurofins seems to be better. Also, our overall experience with Eurofins website, sample submission, email notifications and sequencing results was positive and user friendly. We are looking forward to sequencing with Eurofins.”
“This was really impressive. Cannot believe we can get full plasmid sequencing for $15 a pop. Greatly appreciated.”
“Whole Plasmid Sequencing is good service at a great price point that saves me time. A great addition to the sequencing toolkit that I will be using more in the future.”
“Speed of analysis is exceptional!”
“I have to say, your sequencing is outstanding. From ease of drop off to rapid turn around, to your customer support people if I do have a question and call. I refer everyone to Eurofins. Thanks for doing a great job!”
“Incredibly fast turnaround, and the sequences are stellar. I’m really thankful for your services, they make my job much easier.”
“I am consistently surprised and pleased with how quickly you can provide sequencing data! I dropped my samples off late last evening at my local dropbox and my results were waiting in my inbox when I arrived at work this morning! My PI and I were just joking that we make a comment every time about how the results come so quickly. Maybe we just aren't used to such good customer service. Great job.”
“Love your service. You guys are my go to service, and I constantly recommend you to local folks who do sequencing.”