Eurofins Genomics can provide the primer and probe sequences recommended by the CDC to researchers working on the Monkey Pox Virus. The sequences were publicly provided by the CDC. Researchers can order these primers and probes to create a panel in their lab, following the panel instructions from the CDC. These materials are provided for research purposes only. Eurofins Genomics does not claim that these materials are in any way endorsed or validated by the CDC.
Ordering
There are multiple ways to order the CDC sequences but the easiest is to use the custom DNA oligo order page since it accommodates both modified probes and primers.
CDC Approved Monkey Pox Sequences
*Enter the CDC sequences can be entered on the custom DNA oligo tube order page. Information can change quickly so we encourage all customers to cross check this sequence information against the CDC's documents.
2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes
|
Assay
|
Primer / Probe Sequences
|
Temp (°C)
|
Time (sec)
|
Cycles
|
|
Monkeypox virus Generic
|
Forward
|
5’-GGA AAA TGT AAA GAC AAC GAA TAC AG-3’
|
95
|
20
|
1
|
|
Reverse
|
5’-GCT ATC ACA TAA TCT GGA AGC GTA-3’
|
95
|
3
|
40
|
|
Probe
|
5’-FAM-AAG CCG TAA TCT A<BHQ-1dT>GT TGT CTA TCG TGT CC-Spacer C6-3’
|
60
|
30
|
|
Human DNA (e.g., RNase P)
|
Forward
|
5’-AGA TTT GGA CCT GCG AGC G-3’
|
95
|
20
|
1
|
|
Reverse
|
5’-GAG CGG CTG TCT CCA CAA GT-3’
|
95
|
3
|
40
|
|
Probe
|
5’-FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ1-3’
|
60
|
30
|
Download pre-formatted upload sheet for easy ordering.
References
- Laboratory Procedures | Monkeypox | Poxvirus | CDC Information For Laboratory Personnel | Monkeypox | Poxvirus | CDC
- Laboratory Biological Safety Plan
- Biosafety in Microbiological and Biomedical Laboratories, 6th edition
- Laboratory quality manual
- Regulations and Interpretative guidelines for laboratories and laboratory services
- Laboratory Biosafety Manual
CDC INFORMATION FOR LABORATORIES
Eurofins Genomics is not, and does not claim to be, a primary, preferred or recommended source of the CDC coronavirus primer-probe panel. Eurofins Genomics is independently offering the sequences that were published by the CDC for use in coronavirus detection. These materials are for research use only. They have not been designed, tested, validated by CDC, nor are they in any way endorsed by the CDC or any other public health agency.