I. Standard Vectors

Vectors	Size(bp)	Antibiotic Resistance	Multiple Cloning Site	Sequence	Map	Copy in E. Coli
pUCAmpMinusMCS	3284	Ampicillin	No	Sequence	Map	High
pUCkanMinusMCS	3305	Kanamycin	No	Sequence	Map	High
pUCAmp	3171	Ampicillin	Yes	Sequence	Map	High
pUCKan	3150	Kanamycin	Yes	Sequence	Map	High

II. Custom Vectors

Eurofins Genomics also offers subcloning services into any cloning or expression vector of your choosing. Simply send us the vector into which you want us to subclone your insert. Please contact our technical support team for more information on our custom subcloning services.

When sending a vector for subcloning, please provide 5-10 µg of vector DNA, lyophilized or in solution (conc.: ~200ng/µl). Use a padded envelope and state the order number clearly, and mail your vector and a copy of your quotation or order confirmation to:

Blue Heron Bio
22310 20th Ave SE #100
Bothell, WA 98021 USA

Please include the following information with the submission:

Map of the plasmid
Detailed description of the plasmid (incl. info about sequencing primers)
Complete DNA sequence in text format
If it is an unmodified commercially available plasmid: supplier and info if it is the original plasmid
If it is a modified plasmid: detailed description of the modifications (incl. ab1 files and gel picture)

III. Expression Vectors

Our partner company, Blue Heron Biotech, is pleased to clone into any expression vectors selected by our customers. Setup of custom vectors is simple and easy on our secure website. We also offer OriGene’s PrecisionShuttle™ system for easy subcloning into a large selection of proven destination vectors for a variety of downstream applications. When ordering in Origene expression vectors, customers pay only the cloning and synthesis fee. This eliminates the need to purchase and provide your own vector. Destination vector choices include:

Epitope tags (MYC, DDK, etc.) for protein purification detection
Fluorescent tags (GFP, RFP, YFP, etc.) for protein visualization
Various drug markers (neomycin, puromycin, etc.) for stable cell line establishment
E. coli expression vectors
Custom requests

Destination Vectors

Entry Vectors	SKU	C-Tag	E. coli Selection	Cell Selection	Expression	Maps
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug	PS100001	Myc-DDK	Kanamycin	Neomycin	Mammalian	Map
pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug	PS100063	Myc-DDK	Kanamycin	Neomycin	Mammalian	Map

Expression Vectors

Epitope Tagging	FP Tagging	Section Markers	Lenti / AVV (New)	Bacterial Expression
Epitope tags (MYC, DDK, etc.) for protein purification detection	Fluorescent tags (GFP, RFP, YFP, etc.) for protein visualization	Various drug markers (neomycin, puromycin, etc.) for stable cell line establishment	Adeno-Associated Virus (AAV) vectors used for Gene Therapy	E. coli expression vectors

Destination Vectors	SKU	C-Tag	N-Tag	E. coli	Cell Selection	Expression	Maps
pCMV6-AN-3HA	PS100066		3 HA	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-FC	PS100055		FC	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-FC-S	PS100056		FC	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-3DDK	PS100058		3DDK	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-His	PS100011		His	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-Myc	PS100012		Myc	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-HA	PS100013		HA	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-DDK	PS100014		DDK	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-His-Myc	PS100015		His-Myc	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-Myc-DDK	PS100016		Myc-DDK	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-His-HA	PS100017		His-HA	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-His-DDK	PS100018		His-DDK	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC	PS100020			Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-3HA	PS100067	3 HA		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-3DDK	PS100057	3DDK		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-DDK	PS100005	DDK		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-DDK-His	PS100009	DDK-His		Ampicillin	Neomycin	Mammalian	Map
pCMV6-XL5-DDK-His	PS100068	DDK-His		Ampicillin		Mammalian	Map
pCMV6-AC-FC	PS100053	FC		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-FC-S	PS100054	FC		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-HA	PS100004	HA		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-HA-His	PS100008	HA-His		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AN-GFP-C-His	PS100061	His	tGFP	Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-His	PS100002	His		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-Myc	PS100003	Myc		Ampicillin	Neomycin	Mammalian	Map
pCMV6-AC-Myc-DDK	PS100007	Myc-DDK		Ampicillin	Neomycin	Mammalian	Map
pLenti-C-Myc-DDK	PS100064	Myc-DDK		Chloramphenicol		Mammalian	Map
pLenti-C-Myc-DDK-IRES-Puro	PS100069	Myc-DDK		Chloramphenicol	Puromycin	Mammalian	Map
pLenti-C-Myc-DDK-P2A-Puro	PS100092	Myc-DDK		Chloramphenicol	Puromycin	Mammalian	Map
pLenti-EF1a-C-Myc-DDK-IRES-Puro	PS100085	Myc-DDK		Chloramphenicol	Puromycin	Mammalian	Map
pLenti-C-Myc-DDK-P2A-tGFP	PS100088	Myc-DDK		Chloramphenicol		Mammalian	Map
pAAV-AC-Myc-DDK	PS100089	Myc-DDK		ampicilin		Mammalian	Map
pLenti-C-Myc-DDK-IRES-Neo	PS100081	Myc-DDK		Chloramphenicol	Neomycin	Mammalian	Map
pLenti-C-Myc-DDK-IRES-BSD	PS100082	Myc-DDK		Chloramphenicol	Blasticidin	Mammalian	Map
pCMV6-AC-Myc-His	PS100006	Myc-His		Ampicillin	Neomycin	Mammalian	Map

Destination Vectors	SKU	FP Feature	C-Tag	N-Tag	E. coli Selection	Cell Selection	Maps
pLenti-N-tRFP	PS100077	Red, dimer		tRFP	Chloramphenicol		Map
pLenti-N-tYFP	PS100078	Yellow, dimer		tYFP	Chloramphenicol		Map
pLenti-N-tGFP	PS100073	Green, dimer		tGFP	Chloramphenicol		Map
pCMV6-AC-IRES-GFP-Puro	PS100059	Green, dimer			Ampicillin	Puromycin	Map
pCMV6-AN-FP602	PS100045	Red, dimer		TurboFP602	Ampicillin	Neomycin	Map
pCMV6-AN-FP635	PS100046	Far-red, dimer		TurboFP635	Ampicillin	Neomycin	Map
pCMV6-AN-mKate	PS100047	Far-red, monomer		mKate	Ampicillin	Neomycin	Map
pCMV6-AN-mGFP	PS100048	Green, monomer		mGFP	Ampicillin	Neomycin	Map
pCMV6-AN-mRFP	PS100049	Red, monomer		mRFP	Ampicillin	Neomycin	Map
pCMV6-AN-mYFP	PS100050	Yellow, monomer		mYFP	Ampicillin	Neomycin	Map
pCMV6-AN-mBFP	PS100051	Blue, monomer		mBFP	Ampicillin	Neomycin	Map
pCMV6-AN-mCFP	PS100052	Cyan, monomer		mCFP	Ampicillin	Neomycin	Map
pCMV6-AN-RFP	PS100033	Red,dimer		TurboRFP	Ampicillin	Neomycin	Map
pCMV6-AN-YFP	PS100035	Yellow, dimer		TurboYFP	Ampicillin	Neomycin	Map
pCMV6-AN-GFP	PS100019	Green, dimer		TurboGFP	Ampicillin	Neomycin	Map
pCMV6-A-GFP	PS100026	Green, dimer			Ampicillin		Map
pLenti-C-GFP	PS100065	Green, dimer	GFP		Chloramphenicol		Map
pCMV6-AN-GFP-C-His	PS100061	Green, dimer	His	tGFP	Ampicillin	Neomycin	Map
pCMV6-AC-mBFP	PS100043	Blue, monomer	mBFP		Ampicillin	Neomycin	Map
pCMV6-AC-mCFP	PS100044	Cyan, monomer	mCFP		Ampicillin	Neomycin	Map
pCMV6-AC-mGFP	PS100040	Green, monomer	mGFP		Ampicillin	Neomycin	Map
pLenti-C-mGFP	PS100071	Green, Monomer	mGFP		Chloramphenicol		Map
pLenti-EF1a-C-mGFP	PS100084	Green, Monomer	mGFP		Chloramphenicol		Map
pLenti-C-mGFP-P2A-Puro	PS100093	Green, Monomer	mGFP		Chloramphenicol	Puromycin	Map
pCMV6-AC-mKate	PS100039	Far-red, monomer	mKate		Ampicillin	Neomycin	Map
pCMV6-AC-mRFP	PS100041	Red, monomer	mRFP		Ampicillin	Neomycin	Map
pCMV6-AC-IRES-GFP	PS100027	Green, dimer	Myc-DDK		Ampicillin	Neomycin	Map
pLenti-C-Myc-DDK-P2A-tGFP	PS100088	GFP, dimer	Myc-DDK		Chloramphenicol		Map
pCMV6-AC-mYFP	PS100042	Yellow, monomer	mYFP		Ampicillin	Neomycin	Map
pLenti-EF1a-C-tGFP	PS100072	Green, dimer	tGFP		Chloramphenicol		Map
pAAV-AC-GFP	PS100090	GFP, dimer	tGFP		ampicilin		Map
pAAV-AC-RFP	PS100091	RFP, dimer	tRFP		ampicilin		Map
pLenti-C-tRFP	PS100074	Red, dimer	tRFP		Chloramphenicol		Map
pCMV6-AC-FP602	PS100037	Red, dimer	TurboFP602		Ampicillin	Neomycin	Map
pCMV6-AC-FP635	PS100038	Far-red, dimer	TurboFP635		Ampicillin	Neomycin	Map
pCMV6-AC-GFP	PS100010	Green, dimer	TurboGFP		Ampicillin	Neomycin	Map
pCMV6-AC-RFP	PS100034	Red,dimer	TurboRFP		Ampicillin	Neomycin	Map
pCMV6-AC-YFP	PS100036	Yellow, dimer	TurboYFP		Ampicillin	Neomycin	Map
pLenti-C-tYFP	PS100079	Yellow, dimer	tYFP		Chloramphenicol		Map

Destination Vectors	SKU	C-Tag	E. coli Selection	Cell Selection	Expression	Maps
pCMV6-AC-IRES-GFP-Puro	PS100059		Ampicillin	Puromycin	Mammalian	Map
pCMV6-AC	PS100020		Ampicillin	Neomycin	Mammalian	Map
pCMV6-A-BSD	PS100022		Ampicillin	Blasticidin	Mammalian	Map
pCMV6-A-EM7-BSD	PS100023		Ampicillin/Blasticidin	Blasticidin	Mammalian	Map
pCMV6-A-Hygro	PS100024		Ampicillin	Hygromycin	Mammalian	Map
pCMV6-A-Puro	PS100025		Ampicillin	Puromycin	Mammalian	Map
pCMV6-A-GFP	PS100026		Ampicillin		Mammalian	Map
pLenti-C-mGFP-P2A-Puro	PS100093	mGFP	Chloramphenicol	Puromycin	Mammalian	Map
pCMV6-AC-IRES-GFP	PS100027	Myc-DDK	Ampicillin	Neomycin	Mammalian	Map
pLenti-C-Myc-DDK-IRES-Puro	PS100069	Myc-DDK	Chloramphenicol	Puromycin	Mammalian	Map
pLenti-C-Myc-DDK-P2A-Puro	PS100092	Myc-DDK	Chloramphenicol	Puromycin	Mammalian	Map
pLenti-EF1a-C-Myc-DDK-IRES-Puro	PS100085	Myc-DDK	Chloramphenicol	Puromycin	Mammalian	Map
pLenti-C-Myc-DDK-IRES-Neo	PS100081	Myc-DDK	Chloramphenicol	Neomycin	Mammalian	Map
pLenti-C-Myc-DDK-IRES-BSD	PS100082	Myc-DDK	Chloramphenicol	Blasticidin	Mammalian	Map

Destination Vectors	SKU	C-Tag	N-Tag	E. coli Selection	Cell Selection	Maps
pLenti-N-tRFP	PS100077		tRFP	Chloramphenicol		Map
pLenti-N-tYFP	PS100078		tYFP	Chloramphenicol		Map
pLenti-N-tGFP	PS100073		tGFP	Chloramphenicol		Map
pLenti-C-GFP	PS100065	GFP		Chloramphenicol		Map
pLenti-C-mGFP	PS100071	mGFP		Chloramphenicol		Map
pLenti-EF1a-C-mGFP	PS100084	mGFP		Chloramphenicol		Map
pLenti-C-mGFP-P2A-Puro	PS100093	mGFP		Chloramphenicol	Puromycin	Map
pLenti-C-Myc-DDK	PS100064	Myc-DDK		Chloramphenicol		Map
pLenti-C-Myc-DDK-IRES-Puro	PS100069	Myc-DDK		Chloramphenicol	Puromycin	Map
pLenti-C-Myc-DDK-P2A-Puro	PS100092	Myc-DDK		Chloramphenicol	Puromycin	Map
pLenti-EF1a-C-Myc-DDK-IRES-Puro	PS100085	Myc-DDK		Chloramphenicol	Puromycin	Map
pLenti-C-Myc-DDK-P2A-tGFP	PS100088	Myc-DDK		Chloramphenicol		Map
pAAV-AC-Myc-DDK	PS100089	Myc-DDK		ampicilin		Map
pLenti-C-Myc-DDK-IRES-Neo	PS100081	Myc-DDK		Chloramphenicol	Neomycin	Map
pLenti-C-Myc-DDK-IRES-BSD	PS100082	Myc-DDK		Chloramphenicol	Blasticidin	Map
pLenti-EF1a-C-tGFP	PS100072	tGFP		Chloramphenicol		Map
pAAV-AC-GFP	PS100090	tGFP		ampicilin		Map
pAAV-AC-RFP	PS100091	tRFP		ampicilin		Map
pLenti-C-tRFP	PS100074	tRFP		Chloramphenicol		Map
pLenti-C-tYFP	PS100079	tYFP		Chloramphenicol		Map

Destination Vectors	SKU	C-Tag	N-Tag	E. coli Selection	Expression	Maps
pEX-1	PS100075			Ampicillin	E. coli	Map
pEX-N-His-GST	PS100028		His-GST	Ampicillin	E. coli	Map
pEX-N-GST	PS100029		GST	Ampicillin	E. coli	Map
pEX-N-His	PS100030		His	Ampicillin	E. coli	Map
pEX-C-GST	PS100083	GST		Ampicillin	E. coli	Map
pEX-C-His	PS100076	His		Ampicillin	E. coli	Map

Testimonials

“We have been struggling with oligo quality from other providers. The ones we received from you are far and away the highest quality we have observed and at much higher yields and uniformity than we have seen elsewhere. We are incredibly impressed with the quality and have already started recommending Eurofins to collaborators and other colleagues.”

Andrew A.

Customer

“You make the best primers. I love that you're able to get them to me in 24 hours with next-day shipping on oligos <= 40 bp. Free shipping, too. I've used your service for 4 of our lab's upcoming papers, and the quality is outstanding.”

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“I have been using Eurofins since the past 20 years as a Lab Manager of an academic research lab in Toronto. I have found Eurofins Oligo service to be excellent in all respects. Great job Team Eurofins :)”

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“Best Oligo ordering system I've ever used, and I've been ordering oligos since 1992!”

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“Always a pleasure doing business with you! Excellent service and turnaround times! I have worked with Eurofins for 10 years, ever since I was a graduate student. My current lab used to order oligos and sequencing from other suppliers but I convinced them to switch to Eurofins. I advocate for you regularly to all of my lab colleagues.”

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“Easy to navigate and order oligos. I order multiple oligos at once, each with similar specifications—purification, scale, and more—but with different sequences. The ability to set the default values after entering the first oligo made the process much faster and easier. I especially liked that feature.”

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“Excellent quality oligo synthesis services”

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