Overview

1. Generate a clonal yeast culture
2. Extract and purify genomic DNA
3. Verify DNA concentration and purity
4. Submit sample through the online order page. 

Step 1: prepare yeast pellet

1. Collect 50–100 mg of yeast cells from a fresh, actively growing culture. Ensuring the correct pellet weight and using fresh cells (not from an overgrown culture) is essential. Depending on the yeast species, 50–100 mg of wet cells may correspond to 1.5–2 mL of a dense culture (approximately 8–10 A600 units per mL) and represent roughly 5 × 10⁷ yeast cells in total. Use centrifugation at 500 × g for 2 minutes to pellet the cells.

2. Carefully remove all growth medium (supernatant). Washing with PBS is not required. Verify that the pellet alone weighs 50–100 mg.

3. Resuspend the cell pellet in 0.5 mL of Zymo 1X DNA/RNA Shield and transfer it into a 2 mL screw-cap tube.

Step 2: submit sequencing order online

Place your order on the order page. Make sure to label your samples clearly.

Step 3: physically submit sequencing samples

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For the convenience of our customers, Eurofins Genomics offers DNA extraction services utilizing ZymoBIOMICS DNA extraction kits. This protocol is capable of extracting DNA from various sample types, employing a bead bashing tube to ensure consistent mechanical lysis. However, this method may not be optimal for ONT sequencing, as the resulting read length is often influenced by the input quality.

Eurofins Genomics can accommodate all organisms up to BSL2+ for extraction services; however, we do not accept BSL3 or higher classified organisms.

For samples requiring DNA extraction, we accept aspirated cell pellets shipped frozen on dry ice or growth on sealed, nutrient agar plates shipped at ambient temperature. For microbial DNA isolation submissions, a minimum of 10910^9109 cells is recommended. Please note that we are unable to culture samples; therefore, we encourage you to ensure sample purity and adequate growth before shipping.

While this kit is broadly applicable to many sample types, we cannot guarantee its efficacy for every organism.

Yeast genomics has become an essential tool for understanding cellular biology, metabolic engineering, and industrial fermentation processes. Modern sequencing technologies now enable researchers to characterize entire yeast genomes with unprecedented read length and structural resolution. At our facility, yeast sequencing is performed using platforms developed by Oxford Nanopore Technologies, enabling comprehensive genome analysis of organisms such as Saccharomyces cerevisiae and other industrial or environmental yeast species. This long-read sequencing approach provides a powerful method for resolving complex genomic regions that are difficult to characterize with traditional short-read technologies.

For yeast genomes, these long reads are particularly valuable. Yeast chromosomes often contain repetitive regions, telomeric sequences, and structural rearrangements that can complicate genome assembly using short fragments. Nanopore sequencing enables high-contiguity genome assemblies, accurate structural variant detection, and improved characterization of plasmids, mitochondrial genomes, and chromosomal rearrangements. Also, new technology has made turnaround time faster than ever before.