How to prepare samples?

Sample type

Size Category

Length

Concentration

Min volume

Price per sample

Plasmid

Regular

2.5 - 25 kb

30 ng/uL

≥10 uL

$15

Large

25 - 125 kb

50 ng/uL

≥20 uL

$30

XL

125 - 300 kb

50 ng/uL

≥40 uL

$60

 

How do I submit an order?

It is easy. Go to our online ordering page.

  1. Order in tube format
  2. Order in plate format

How do I ship samples?

1. Dropbox - we have a nationwide network of dropboxes. If you want a box for your lab, let us know! Email GenomicsSupport@eurofins.com to request a box.

2. Free Digital Shipping Labels - digital shipping labels are provided free for a vast majority of sequencing orders. You can select the option for digital shipping label during checkout. Take a look at our sample submission options page for more details.

3. Ship the samples using your normal carrier - If using your own carrier, we highly recommend shipping overnight/next-day delivery to ensure the samples do not degrade in transit.

How do I get results?

Results are available to download from the order history page. You will be emailed when the results are ready as well. Currently, over 90% of results are delivered by 5 pm ET the same day the samples are received.

What Type of Sequencing Should I Use?

There are pros and cons of every sequencing method. Sanger sequencing and Oxford Nanopore sequencing (ONT) are both methods for determining the sequence of nucleotides in a piece of DNA. Typically Sanger is consider the most accurate method for short-read sequencing and NGS is better for long-read sequencing.

Overall, the choice between Sanger sequencing and ONT will depend on the specific needs of the application. Both methods have their strengths and limitations, and the appropriate method will depend on factors such as the length and quality of the DNA sample, the desired level of accuracy, and the cost and availability of the necessary equipment.

 

Pros

Cons

Short read sequencing

(Sanger)
  • Widely used and well-established method
  • High accuracy and precision
  • Can be automated for high-throughput sequencing
  • Chromatogram makes it easy to visually interpret results
  • Limited read length (typically up to 1000 base pairs)
  • Requires relatively large amounts of high-quality DNA
  • Requires reassembly for covering longer regions.
  • Difficult to sequence repetitive regions without gaps and resolve large variations.

Long read sequencing

(Whole Plasmid)
  • Long read lengths (up to several hundred thousand base pairs)
  • Can sequence a wide range of sample types, including low-quality and degraded DNA
  • No primer design required
  • All data from one sample

 
  • Lower accuracy compared to Sanger sequencing, particularly for shorter reads
  • More expensive per base pair compared to Sanger sequencing
  • Cannot resolve single bases.

 

 

Fast, accurate, and affordable long-read sequencing 

Confirm your full plasmids faster, more accurately, and more affordably than ever before. Whole genome sequencing offers long-read sequencing from one sample. It is suitable for large fragment DNA samples. NGS generation 3 technology has made it possible to quickly sequence whole plasmids in a fraction of the time and without the hassle of having to design and synthesize primers. Eurofins has developed a simple, user-friendly ordering process for sequencing plasmids and turning around results quickly.

Order in tube format

Order in plate format

Benefits

  • Long read recovery of several kilobases
  • Fast TAT compared to traditional primer walking and plasmid verification techniques
  • Lower cost than traditional primer walking techniques
  • Verification of the entire vector sequence
  • Scalable--verify large plasmid constructs and large number of samples without impacting turnaround time.
  • No primer design required. Easy set up.
  • Available from one sample
  • 5 - 4 million reads per Flow Cell (dependent on DNA quality and length)

Applications

Whole plasmid sequencing using NGS Gen 3 technology is a powerful and accurate method for characterizing and analyzing plasmids, which make it well suited for a wide range of applications.

  • Plasmid Verification
  • Resequencing of whole genomes
  • Assembly of genomes
  • Identification of taxonomic background
  • Metagenomic analysis of long reads

Technology

Novel Nanopore technology is used to obtain very long sequences of several kilobases. It is a powerful method for characterizing and analyzing plasmids, which are small, circular pieces of DNA that can replicate independently of the genome. There are several key benefits to using ONT for whole plasmid sequencing:

  1. High throughput: ONT allows for high-throughput sequencing of multiple samples in a single run, making it ideal for large-scale studies and projects.
  2. Long read lengths: ONT generates long, continuous reads, which are particularly useful for sequencing large plasmids or for assembling complex plasmid structures.
  3. High accuracy: ONT has a high accuracy rate, making it suitable for a wide range of applications, including plasmid engineering, quality control, and the identification of genetic mutations.
  4. High resolution: ONT provides high-resolution data, allowing researchers to accurately identify and characterize even subtle differences in plasmid sequences.
  5. Versatility: ONT is suitable for a wide range of sample types, including low-quality and degraded DNA, making it a versatile tool for plasmid analysis.