Premium Upgrade Service

The Premium PCR service is designed for full-length, fragmentation-free sequencing of linear, double-stranded DNA ranging from 100 bp to 25 kb. It serves as an enhanced alternative to the standard Linear/PCR service, which may introduce minimal fragmentation during library preparation.

When to Use Premium PCR:

• Diverse Molecular Mixtures: Ideal if your linear DNA sample contains a mixture of molecules (e.g., barcode or variant libraries) that require comprehensive characterization.
• Fragmentation-Free Reads: Suitable when you need full-length, end-to-end sequencing without any fragmentation.
• High Read Count: Delivers up to 5,000 raw reads per sample, significantly exceeding the output of the standard Linear/PCR service.
• Enhanced Accuracy: Provides raw reads with greater per-base confidence compared to the regular service.
• Complete Data Retrieval: Includes all raw reads generated from your sample, not limited to those aligning with your consensus sequence. (Note: While all reads are returned, a small chance of demultiplexing errors exists, meaning a few reads from your sample might be shared with others in the same sequencing run.)

Technology and Workflow:

This service uses cutting-edge long-read sequencing technology from Oxford Nanopore Technologies and includes:

1. Amplification-Free Library Preparation: Your input DNA is preserved intact, with no fragmentation, using the latest v14 library prep chemistry, including end-ligation for linear DNA.
2. Sequencing with High Accuracy: Libraries are sequenced on R10.4.1 flow cells with a primer-free protocol, and raw data is provided in .fastq.gz format.
3. Consensus Generation: Raw reads are aligned to produce a high-accuracy linear consensus sequence for the most abundant molecular species in your sample. For samples with multiple species, you can analyze the raw reads independently or request additional consensus sequences by contacting support@plasmidsaurus.com.

 ADD THE DATA FILE TYPES STUFF

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Pricing

Description	Catalog #	Estimated data output	Price per sample	Turnaround time
Ligation Sequencing - Medium	3010-ONTLSM	200 Mbp Long Reads	$240	3 business days
Ligation Sequencing - Large	3010-ONTLSL	400 Mbp Long Reads	$315	3 business days
Ligation Sequencing - XL	3010-ONTLSXL	Min 900 Mbp Long Reads	$420	3 business days

 

 

Specifications

Interpreting Results

If you need help interpretation your results, please visit the results interpretation page.